## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5397-Z0085-CTCTATGTTGCTGAT/441525-L5397-Z0085-CTCTATGTTGCTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5397-Z0085-CTCTATGTTGCTGAT/441525-L5397-Z0085-CTCTATGTTGCTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:52:23 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000964	1000964	195	195577947	162580051	0.83128	133557806	0.682888	287405038	287405038

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2030827	26.24072	26.25	26.25	26.25
1	196372	33.962088	34.25	34.25	35
2	3178	34.319069	35	34.25	35
3	1114529	34.090936	35	34.25	35
4	7220	31.700807	31.75	30	33.5
5	8314	34.774844	35	35	35
6	2817711	32.350385	32.5	31	35
7	10358	32.213355	32.5	30	35
8	13287	33.277419	33.75	32.5	35
9	4835963	33.369607	34.25	32.5	35
10	6075	34.196689	35	34.25	35
11	13095	32.563701	33.75	31.75	35
12	5900554	32.997198	35	32.5	35
13	32411	32.892343	35	32.5	35
14	591243	32.976061	35	32.5	35
15	4390859	32.923225	35	32.5	35
16	587252	32.977421	35	32.5	35
17	5046232	32.89667	35	32.5	35
18	93	32.875705	35	32.5	35
19	5392323	32.859996	35	32.5	35
20	251523	32.791726	35	32.5	35
21	269876	32.677765	35	32	35
22	2970555	32.442514	35	31.75	35
23	0	32.092215	34.25	31.75	35
24	29506	31.551143	34.25	31	35
25	24833953	30.739479	33.75	30	35
26	0	30.23446	33.75	29.25	35
27	23	29.544684	33.75	27.5	35
28	178607	28.850479	33.5	25	35
29	488202	28.202103	32.5	22.5	35
30	4748759	27.630436	32.5	20.25	35
31	0	27.119172	31.75	19	35
32	8960502	26.601421	30.75	18.25	35
33	0	26.165369	29.25	17.75	35
34	0	25.828599	27.75	17.75	35
35	119848545	25.472773	26.25	17.5	34.25
36	0	25.305792	25	17.5	34.25
37	0	24.989409	25	17.5	34.25
38	0	24.804541	25	17.25	33.75
39	0	24.625135	25	17.25	33.75
40	0	24.382853	25	17	33.75
41	0	24.248827	25	17	33.75
42	0	24.071869	25	16.75	33.5
43	0	23.952758	24.5	16.75	33.5
44	0	23.868007	24.25	16.666667	33.5
45	0	23.81343	24.25	16.5	33.5
46	0	23.757696	24	16.5	33.5
47	0	23.78064	24	16.5	33.25
48	0	23.824085	24.25	16.5	33.25
49	0	23.82463	24.25	16.5	33
50	0	23.803766	24.25	16.75	33
51	0	23.73287	23.5	16.75	32.5
52	0	23.717744	23.5	16.75	32.5
53	0	23.537981	23.5	16.75	32.5
54	0	23.364652	23.5	16.5	32.5
55	0	23.180283	23	16.5	31.75
56	0	23.217814	23.5	16.5	31.75
57	0	23.145146	23.25	16.75	31.75
58	0	23.205691	23.5	17	31.25
59	0	23.08895	23.5	17	31
60	0	23.041492	23.5	17	30.25
61	0	23.040242	23.5	17	30
62	0	23.042588	23.5	17.25	30
63	0	23.053319	23.5	17.5	30
64	0	23.100365	23.5	17.75	29.75
65	0	23.204018	23.5	18	30
66	0	23.291426	24	18.25	30
67	0	23.350422	24.5	18.25	30
68	0	23.43331	25	18.5	30
69	0	23.485256	25	18.5	30
70	0	23.573529	25	18.5	30
71	0	23.602717	25	18.75	30
72	0	23.649777	25	19	30
73	0	23.694394	25	19	30
74	0	23.656018	25	19	30
75	0	23.553897	25	18.5	30
76	0	23.440311	25	18.5	30
77	0	23.195106	25	17.5	30
78	0	22.960981	25	17	30
79	0	22.888814	25	16.75	30
80	0	22.979543	25	17	30
81	0	23.02744	25	17	30
82	0	23.112554	25	17.25	30
83	0	23.148572	25	17.5	30
84	0	23.233793	25	17.5	30
85	0	23.345823	25	17.75	30
86	0	23.090464	24.5	17.25	30
87	0	22.812773	24.25	16.75	30
88	0	22.88482	24.25	16.75	30
89	0	22.950315	24.25	16.75	30
90	0	22.850593	24	16.75	29.5
91	0	22.905878	24.25	17	29.75
92	0	23.007557	23.75	17	29.25
93	0	23.11331	24	17.25	30
94	0	23.231998	24	17.25	30
95	0	22.844419	23.5	16.75	29.75
96	0	22.591347	23	16.25	29.25
97	0	22.615361	23	16.5	29.25
98	0	22.484839	22.75	16.5	29.25
99	0	22.332771	22.75	16.25	29.25
100	0	22.084587	22.5	16	28.5
101	0	21.828286	22	15.5	28
102	0	21.617307	21.5	15.5	27.5
103	0	21.204436	21	15.5	26.75
104	0	20.520711	19.75	14.75	26
105	0	19.730907	19.5	14.25	25
106	0	18.608877	18	13.25	23.5
107	0	17.296219	16.75	12	22
108	0	9.926263	9.5	6	12.5