## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5396-Z0083-CAGTGTATTGCTGAT/441525-L5396-Z0083-CAGTGTATTGCTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5396-Z0083-CAGTGTATTGCTGAT/441525-L5396-Z0083-CAGTGTATTGCTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:57 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
998354	998354	193	193447399	160227810	0.828276	130403015	0.674101	282970324	282970324

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2026439	26.241721	26.25	26.25	26.25
1	206117	33.977566	34.25	33.5	35
2	3325	34.353878	35	34.25	35
3	1175977	33.960274	34.25	32.5	35
4	6942	31.267098	31.75	30	33.5
5	8291	34.700133	35	35	35
6	2838376	33.231647	34.25	32.5	35
7	10149	32.284687	32.5	30.25	35
8	13824	33.200794	33.75	32.5	35
9	4878438	33.35105	34.25	32.5	35
10	6414	34.192353	35	34.25	35
11	13898	32.340031	33.75	31.75	35
12	5946923	32.745424	35	31.75	35
13	34218	32.641162	35	31.75	35
14	602772	32.752165	35	32.25	35
15	4408415	32.711856	35	31.75	35
16	597064	32.792513	35	32.5	35
17	5034924	32.711751	35	31.75	35
18	103	32.704743	35	31.75	35
19	5406980	32.675729	35	31.75	35
20	265107	32.594566	35	31.75	35
21	265941	32.483094	35	31.75	35
22	3059750	32.231242	34.25	31.75	35
23	0	31.868777	34.25	31	35
24	30003	31.298219	34.25	30.25	35
25	25544052	30.442281	33.75	29.25	35
26	0	29.91885	33.75	28	35
27	20	29.194532	33.5	26.25	35
28	175419	28.472524	33	23.75	35
29	484503	27.824931	32.5	21	35
30	4678383	27.270241	31.75	19.5	35
31	0	26.785354	31.25	18.5	35
32	9063894	26.306201	30	18	35
33	0	25.896463	28.5	17.75	35
34	0	25.602316	27.25	17.5	34.25
35	116660738	25.281365	25.25	17.5	34.25
36	0	25.119114	25	17.5	34.25
37	0	24.801684	25	17.25	33.75
38	0	24.618003	25	17.25	33.75
39	0	24.405532	25	17.25	33.75
40	0	24.148477	25	17	33.5
41	0	24.006615	25	16.75	33.5
42	0	23.838335	24	16.75	33
43	0	23.724409	23.5	16.5	33
44	0	23.619151	23.5	16.5	32.75
45	0	23.560558	23.5	16.25	32.75
46	0	23.496054	23.5	16.25	32.5
47	0	23.528139	23.5	16.25	32.5
48	0	23.557559	23.5	16.5	32.5
49	0	23.507363	23.5	16.5	32.5
50	0	23.498797	23.5	16.5	32.5
51	0	23.414223	23.5	16.5	32.5
52	0	23.401334	23.5	16.75	32
53	0	23.218863	23	16.5	31.75
54	0	23.037658	23	16.5	31.75
55	0	22.874674	23	16.25	31.25
56	0	22.936151	23	16.5	31
57	0	22.891777	23	16.5	30.5
58	0	22.946077	23	16.75	30
59	0	22.872029	23	16.75	30
60	0	22.819379	23	17	30
61	0	22.830579	23	17	30
62	0	22.92006	23.5	17.25	30
63	0	22.962955	23.5	17.5	29.5
64	0	23.018483	23.5	17.75	29.25
65	0	23.154108	23.5	18	29.25
66	0	23.219529	24	18	30
67	0	23.252071	24.5	18.25	30
68	0	23.389697	25	18.5	30
69	0	23.509468	25	18.5	30
70	0	23.574471	25	18.5	30
71	0	23.648297	25	18.75	30
72	0	23.676907	25	19	30
73	0	23.69807	25	19	30
74	0	23.737873	25	19	30
75	0	23.571199	25	18.5	30
76	0	23.44196	25	18.5	30
77	0	23.259279	25	17.5	30
78	0	22.939572	25	16.75	30
79	0	22.82732	25	16.5	30
80	0	22.971708	25	16.75	30
81	0	23.096949	25	17	30
82	0	23.186918	25	17.25	30
83	0	23.201732	25	17.5	30
84	0	23.224831	25	17.5	30
85	0	23.316764	25	17.75	30
86	0	23.117216	25	17	30
87	0	22.959828	24.75	16.75	30
88	0	22.985035	24.5	16.75	30
89	0	22.991949	24.5	16.75	30
90	0	22.967108	24.25	17	30
91	0	23.050693	24.25	17	30
92	0	23.076667	24.25	17.25	30
93	0	23.358847	24.5	17.25	30
94	0	23.361515	24.25	17.25	30
95	0	23.205575	23.75	17	30
96	0	23.083568	23.5	17	30
97	0	22.966481	23.5	16.75	30
98	0	22.901497	23.5	16.666667	30
99	0	22.593428	23	16.5	29.25
100	0	22.455886	22.75	16.25	29.25
101	0	21.99274	22	16	28.5
102	0	21.670202	21.75	16	27.5
103	0	21.358135	21	15.25	27.5
104	0	21.170909	20.75	15.25	26.75
105	0	20.025657	19.5	14.5	25
106	0	19.074343	18.75	13.5	24.5
107	0	17.80596	17.5	12.5	22.5
108	0	9.851968	9.333333	6	12.5