## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5391-Z0077-CAATGACTATCTGAT/441525-L5391-Z0077-CAATGACTATCTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5391-Z0077-CAATGACTATCTGAT/441525-L5391-Z0077-CAATGACTATCTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:20 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000978	1000978	176	176325606	143972427	0.816515	116394897	0.660113	255213750	255213750

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2030700	26.242871	26.25	26.25	26.25
1	216373	34.234231	34.25	34.25	35
2	2746	34.446744	35	34.25	35
3	1157138	33.823673	34.25	32.5	35
4	5028	31.342775	31.75	30	33.5
5	7328	34.693383	35	35	35
6	2825772	32.770918	34.25	31.75	35
7	8802	32.078555	32.5	30	35
8	12712	33.388373	33.75	32.5	35
9	4874373	33.438282	34.25	32.5	35
10	5800	34.068726	35	34.25	35
11	13124	32.112323	33.75	31.25	35
12	5810050	32.452423	35	31.75	35
13	33980	32.483464	34.25	31.75	35
14	593739	32.598305	35	31.75	35
15	4255359	32.550546	35	31.75	35
16	576567	32.635511	35	31.75	35
17	4822517	32.581305	35	31.75	35
18	84	32.553183	35	31.75	35
19	5100987	32.496369	35	31.75	35
20	260475	32.393095	34.25	31.75	35
21	230157	32.192332	34.25	31.25	35
22	2826484	31.836378	34.25	31	35
23	0	31.27001	34.25	30	35
24	27016	30.457701	33.75	28.75	35
25	23677174	29.242775	33.5	26.5	35
26	0	28.562614	32.5	24.75	35
27	6	27.662923	32.25	21.333333	35
28	144432	26.806688	31.5	18.5	35
29	411786	26.070123	30	17	35
30	4000522	25.466195	28.5	16.5	34.25
31	0	24.961891	26.5	16.25	34.25
32	8568634	24.495337	25	16	33.75
33	0	24.118135	25	16	33.75
34	0	23.839363	24.25	16.25	33
35	103825741	23.51163	23.5	16.25	32.5
36	0	23.369246	23.5	16.25	31.75
37	0	23.017083	23	16.25	31.25
38	0	22.762252	23	16	30.25
39	0	22.533471	22.5	16	30
40	0	22.209655	22	15.75	29.75
41	0	22.00645	21.75	15.5	29.25
42	0	21.773153	21.5	15.25	28.5
43	0	21.570988	21	15.25	27.75
44	0	21.437495	21	15	27.5
45	0	21.380241	21	15	27.5
46	0	21.323808	21	15	27.5
47	0	21.409673	21	15.25	27.5
48	0	21.456324	21	15.25	27.5
49	0	21.428121	21	15.25	27.5
50	0	21.410188	21	15.25	27.5
51	0	21.335356	21	15.5	27
52	0	21.371204	21.25	15.5	26.75
53	0	21.227108	21	15.5	26.75
54	0	21.133244	21	15.25	26
55	0	21.078422	21	15.25	26
56	0	21.229801	21	15.5	26.25
57	0	21.294468	21.5	15.5	26.75
58	0	21.51022	21.75	15.75	27.5
59	0	21.571436	21.75	16	27.5
60	0	21.692999	22	16	27.5
61	0	21.842892	22.25	16.25	27.5
62	0	22.047441	22.5	16.5	27.5
63	0	22.267014	23	17	27.75
64	0	22.486513	23	17.25	28.5
65	0	22.686781	23.5	17.25	29.25
66	0	22.971385	23.5	17.5	30
67	0	23.146348	24.5	17.75	30
68	0	23.340044	25	18	30
69	0	23.575046	25	18.25	30
70	0	23.798297	25	18.5	30
71	0	23.88952	25	18.75	30
72	0	24.165455	25	19.25	30
73	0	24.29089	25	19.5	30
74	0	24.36917	25	19.5	30
75	0	24.359754	25	19.5	30
76	0	24.354231	25	19.5	30
77	0	24.262939	25	19	30.25
78	0	24.086445	25	18.5	30.5
79	0	24.082882	25.5	18.5	30.25
80	0	24.253264	25.5	18.75	30.5
81	0	24.387084	25.75	19.25	31
82	0	24.295392	25.5	19	30.5
83	0	24.431463	25.5	19.5	31
84	0	24.463037	25.5	19.5	30.5
85	0	24.323214	25.5	19.25	30
86	0	24.151414	25	18.75	30
87	0	23.924014	25	18.5	30
88	0	23.975074	25	18.5	30
89	0	23.951703	25	18.75	30
90	0	23.840331	25	18.5	30
91	0	23.988788	25	18.75	30
92	0	23.960666	25	18.75	30
93	0	24.188131	25	18.75	30.25
94	0	23.873735	25	18.25	30
95	0	23.893866	25	18.25	30
96	0	23.45061	24.5	17.5	30
97	0	23.276857	23.75	17.25	30
98	0	22.925854	23.5	17	29.25
99	0	23.103994	23.75	17	29.75
100	0	22.987071	23	17	30
101	0	22.662035	22.75	16.5	29.25
102	0	22.073749	22	15.75	29.25
103	0	21.910714	21.75	16	27.5
104	0	21.237855	21	15.25	27
105	0	19.966677	19.5	14.25	25
106	0	19.069757	18.75	13.75	24.25
107	0	18.074496	17.5	13	23
108	0	9.746987	9.333333	6	12.5