## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5389-Z0075-CTATGCACCATCGAT/441525-L5389-Z0075-CTATGCACCATCGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5389-Z0075-CTATGCACCATCGAT/441525-L5389-Z0075-CTATGCACCATCGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:21 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999383	999383	207	207650485	174478537	0.840251	143334074	0.690266	307118679	307118679

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2030273	26.234622	26.25	26.25	26.25
1	191363	34.609723	35	35	35
2	4273	34.431679	35	34.25	35
3	1119277	33.982991	34.25	32.5	35
4	8774	32.179593	32.5	31.75	34.25
5	9803	34.621963	35	35	35
6	2736613	32.176917	32.5	30.25	35
7	12303	32.278366	33	30.25	35
8	15279	33.129321	33.75	32.5	35
9	4803424	33.316028	34.25	32.5	35
10	7026	34.014336	35	34.25	35
11	15082	32.362279	33.75	31.75	35
12	5891076	32.871771	35	32.5	35
13	37725	32.845281	35	32.5	35
14	607032	32.93689	35	32.5	35
15	4414689	32.903568	35	32.5	35
16	617190	33.018289	35	32.5	35
17	5085283	32.920707	35	32.5	35
18	96	32.918338	35	32.5	35
19	5565367	32.900879	35	32.5	35
20	277982	32.869953	35	32.5	35
21	310164	32.851615	35	32.5	35
22	3352407	32.688451	35	32.25	35
23	0	32.518211	35	31.75	35
24	33699	32.236521	34.25	31.75	35
25	26421462	31.706527	34.25	31.25	35
26	0	31.459491	34.25	31	35
27	15	30.966378	34.25	30	35
28	197771	30.419577	33.75	29.25	35
29	550963	29.844391	33.75	28	35
30	5293082	29.276135	33.75	26	35
31	0	28.720243	33.5	24.25	35
32	9416134	28.157415	32.5	22	35
33	0	27.670827	32.5	20.5	35
34	0	27.308758	31.75	20	35
35	128624858	26.950773	31	19.5	35
36	0	26.780264	30	19.5	35
37	0	26.445154	29.25	19	35
38	0	26.180498	28	19	35
39	0	25.881223	27.5	18.5	34.25
40	0	25.538601	26	18.25	34.25
41	0	25.277543	25.25	18	34.25
42	0	24.986455	25	17.75	33.75
43	0	24.741152	25	17.5	33.75
44	0	24.512774	25	17.25	33.75
45	0	24.365457	25	17	33.75
46	0	24.217498	25	17	33.5
47	0	24.153877	25	17	33.5
48	0	24.120976	25	17	33.25
49	0	24.034166	25	17	33
50	0	23.960021	25	17	32.5
51	0	23.800047	24	17	32.5
52	0	23.728558	23.5	17	32.5
53	0	23.514377	23.5	17	31.75
54	0	23.298547	23.5	16.75	31.75
55	0	23.087152	23.25	16.5	31.5
56	0	23.114168	23.5	16.75	31.25
57	0	23.047805	23.25	16.75	31
58	0	23.093936	23.5	17	30.5
59	0	23.021286	23.5	17	30
60	0	22.998118	23.5	17	30
61	0	22.986511	23.5	17.25	30
62	0	23.023267	23.5	17.5	30
63	0	23.064959	23.5	17.75	30
64	0	23.136632	23.5	18	29.75
65	0	23.209894	23.5	18	30
66	0	23.300506	24.25	18.25	30
67	0	23.370749	25	18.25	30
68	0	23.45056	25	18.25	30
69	0	23.575526	25	18.5	30
70	0	23.617971	25	18.5	30
71	0	23.653654	25	18.75	30
72	0	23.719128	25	19	30
73	0	23.736995	25	19	30
74	0	23.741136	25	19	30
75	0	23.690536	25	18.75	30
76	0	23.513835	25	18.5	30
77	0	23.302603	25	17.5	30
78	0	23.02587	25	17	30
79	0	22.972643	25	16.75	30
80	0	23.151092	25	17	30
81	0	23.240134	25	17.25	30
82	0	23.247866	25	17.5	30
83	0	23.227598	25	17.5	30
84	0	23.320397	25	17.75	30
85	0	23.236514	25	17.5	30
86	0	23.075633	24.25	17.25	30
87	0	22.94962	24.25	17	30
88	0	23.007987	24.25	17	30
89	0	22.924137	24.25	17	30
90	0	22.911315	23.5	17	29.25
91	0	22.905753	23.5	17	29.25
92	0	22.887746	23.75	17	29.25
93	0	23.138461	24.25	17.25	30
94	0	23.06872	23.5	17	30
95	0	22.895346	23.5	16.75	30
96	0	22.666705	23	16.666667	29.25
97	0	22.589799	23	16.25	29.25
98	0	22.445087	23	16.25	29.25
99	0	22.509808	22.75	16.5	29.25
100	0	22.303292	22.75	16.5	28.5
101	0	21.654598	21.75	15.5	27.5
102	0	21.223627	21	15	27.5
103	0	20.989923	20.75	15.25	26.75
104	0	20.349824	19.5	14.75	25.5
105	0	19.499412	19	14	25
106	0	18.554189	17.75	13.25	23.5
107	0	17.488703	17	12.5	22
108	0	9.901299	9.5	6	12.5