## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5388-Z0074-CTATGAATGAGCGAT/441525-L5388-Z0074-CTATGAATGAGCGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5388-Z0074-CTATGAATGAGCGAT/441525-L5388-Z0074-CTATGAATGAGCGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:03 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999825	999825	201	201689159	168825822	0.837059	139241607	0.690377	297880611	297880611

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2032207	26.242743	26.25	26.25	26.25
1	213531	34.600964	35	34.25	35
2	3753	34.367356	35	34.25	35
3	1165528	33.995023	34.25	34.25	35
4	8336	31.8388	31.75	30	33.5
5	9332	34.683838	35	35	35
6	2753200	32.755284	34.25	31.75	35
7	11335	32.260891	32.5	30.25	35
8	15007	33.304033	33.75	32.5	35
9	4806315	33.436237	34.25	32.5	35
10	6936	34.256409	35	34.25	35
11	15130	32.504916	33.75	31.75	35
12	5860280	32.900875	35	32.5	35
13	37284	32.82068	35	32.5	35
14	609542	32.916313	35	32.5	35
15	4337308	32.885091	35	32.5	35
16	599803	32.958217	35	32.5	35
17	4970941	32.878604	35	32.5	35
18	162	32.889387	35	32.5	35
19	5407407	32.8634	35	32.5	35
20	276520	32.828507	35	32.5	35
21	295490	32.749641	35	32.5	35
22	3123067	32.609256	35	31.75	35
23	0	32.392858	35	31.75	35
24	32329	32.05841	34.25	31.75	35
25	25121072	31.492203	34.25	31	35
26	0	31.133248	34.25	30.25	35
27	24	30.609989	34.25	29.5	35
28	205396	30.032878	33.75	28.5	35
29	530317	29.440762	33.75	26.75	35
30	4901003	28.859517	33.5	25	35
31	0	28.29335	33	22.75	35
32	8990795	27.711111	32.5	20.666667	35
33	0	27.205375	31.75	19.5	35
34	0	26.801194	31	18.75	35
35	125349809	26.410921	30	18.5	35
36	0	26.223742	29	18.5	35
37	0	25.887886	27.5	18.25	35
38	0	25.608782	26.25	18	34.25
39	0	25.338923	25	18	34.25
40	0	25.020134	25	17.75	34.25
41	0	24.761782	25	17.25	33.75
42	0	24.471545	25	17	33.75
43	0	24.246278	25	17	33.75
44	0	24.02825	25	16.75	33.5
45	0	23.882922	24.5	16.5	33.5
46	0	23.760209	23.75	16.5	33.25
47	0	23.711261	23.5	16.5	33
48	0	23.698502	23.5	16.5	32.75
49	0	23.627619	23.5	16.5	32.5
50	0	23.567421	23.5	16.75	32.5
51	0	23.435736	23.5	16.75	32.5
52	0	23.377358	23.5	16.75	31.75
53	0	23.166307	23	16.5	31.75
54	0	22.977276	23	16.5	31.5
55	0	22.766871	23	16.25	31
56	0	22.806024	23	16.5	30.5
57	0	22.776083	23	16.5	30
58	0	22.852972	23	16.75	30
59	0	22.760174	23	16.75	30
60	0	22.73804	23	17	30
61	0	22.778172	23	17	30
62	0	22.835041	23.5	17.25	29.75
63	0	22.915818	23.5	17.5	29.25
64	0	23.012048	23.5	17.75	29.25
65	0	23.113852	23.5	18	29.25
66	0	23.232058	24	18	30
67	0	23.285421	24.5	18	30
68	0	23.36684	25	18.25	30
69	0	23.519206	25	18.5	30
70	0	23.640375	25	18.5	30
71	0	23.685227	25	18.75	30
72	0	23.769544	25	19	30
73	0	23.806342	25	19.25	30
74	0	23.800425	25	19	30
75	0	23.678605	25	18.5	30
76	0	23.53279	25	18.25	30
77	0	23.340978	25	17.5	30
78	0	23.180829	25	17	30
79	0	23.08846	25	16.75	30
80	0	23.19074	25	17	30
81	0	23.323735	25	17.25	30
82	0	23.479874	25	17.75	30
83	0	23.488071	25	17.75	30
84	0	23.587215	25	18	30
85	0	23.51947	25	17.75	30
86	0	23.379564	25	17.5	30
87	0	23.108391	24.75	17	30
88	0	23.154856	24.75	17	30
89	0	23.305167	25	17.25	30
90	0	23.255878	24.5	17.25	30
91	0	23.31071	24.5	17.5	30
92	0	23.304584	24.5	17.5	30
93	0	23.588864	25	17.75	30
94	0	23.48834	24.25	17.5	30
95	0	23.335425	24.25	17.25	30
96	0	23.080541	23.5	17	30
97	0	22.938972	23.5	16.75	30
98	0	22.789657	23.25	16.666667	30
99	0	22.808932	23.5	17	29.25
100	0	22.561283	22.75	16.666667	29.25
101	0	22.199034	22.5	16.25	29
102	0	21.842042	21.75	16	28.5
103	0	21.396029	21	15.5	27.25
104	0	20.9094	20.5	15.25	26.25
105	0	19.931616	19.5	14.25	25
106	0	19.023264	18.5	14	24
107	0	17.920069	17.75	12.75	22.75
108	0	10.068769	9.5	6	12.5