## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5386-Z0071-CGTGCGCAAGCAGAT/441525-L5386-Z0071-CGTGCGCAAGCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5386-Z0071-CGTGCGCAAGCAGAT/441525-L5386-Z0071-CGTGCGCAAGCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:57 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
998503	998503	218	218177623	185260601	0.849127	153833117	0.705082	325338621	325338621

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2030616	26.239357	26.25	26.25	26.25
1	196460	34.444911	35	34.25	35
2	5193	34.628652	35	34.25	35
3	1206425	34.52463	35	34.25	35
4	9621	33.093222	33.5	31.75	34.25
5	11410	34.798128	35	35	35
6	2766011	32.771561	34.25	31.75	35
7	13052	32.665774	33.75	31	35
8	17562	33.212982	33.75	32.5	35
9	4772390	33.437343	34.25	32.5	35
10	7855	34.110089	35	34.25	35
11	17079	32.561238	33.75	31.75	35
12	5839960	32.938051	35	32.5	35
13	42932	32.876995	35	32.5	35
14	619071	32.955492	35	32.5	35
15	4344990	32.89981	35	32.5	35
16	624922	32.952398	35	32.5	35
17	4948539	32.86734	35	32.5	35
18	123	32.881332	35	32.5	35
19	5442811	32.860846	35	32.5	35
20	311351	32.821576	35	32.5	35
21	356601	32.771041	35	32.5	35
22	3549674	32.662893	35	31.75	35
23	0	32.504631	35	31.75	35
24	38088	32.229223	34.25	31.75	35
25	26315013	31.812635	34.25	31.25	35
26	0	31.512857	34.25	31	35
27	36	31.100905	34.25	30.5	35
28	231310	30.653574	33.75	30	35
29	625411	30.185218	33.75	28.75	35
30	5868416	29.719488	33.75	27.5	35
31	0	29.274039	33.75	26	35
32	9747865	28.812117	33.5	24.5	35
33	0	28.408674	33	23	35
34	0	28.097327	32.5	22	35
35	138216836	27.780724	32.5	21	35
36	0	27.643213	31.75	21	35
37	0	27.353797	31.75	20.5	35
38	0	27.120988	31	20.25	35
39	0	26.889912	30	20	35
40	0	26.608219	30	19.5	35
41	0	26.409363	29.25	19	35
42	0	26.178949	28.75	18.75	35
43	0	26.003028	28.5	18.5	34.25
44	0	25.845876	27.75	18.5	34.25
45	0	25.733063	27.5	18.5	34.25
46	0	25.626901	27.5	18.25	34.25
47	0	25.595309	27.5	18.25	34.25
48	0	25.556517	27.25	18.25	34.25
49	0	25.47586	26.75	18.25	34.25
50	0	25.420129	26.5	18.25	34.25
51	0	25.290177	26	18.25	33.75
52	0	25.239105	25.5	18.25	33.75
53	0	25.020932	25	18	33.75
54	0	24.799308	25	17.75	33.75
55	0	24.541475	25	17.5	33.75
56	0	24.534308	25	17.75	33.5
57	0	24.42423	25	17.75	33.5
58	0	24.380328	25	18	33
59	0	24.223553	25	17.75	32.5
60	0	24.097035	25	17.75	32.5
61	0	24.032339	25	18	31.75
62	0	24.000155	25	18.25	31.75
63	0	23.987284	25	18.5	31
64	0	23.999135	25	18.75	30.25
65	0	24.054375	25	19	30
66	0	24.129082	25	19	30
67	0	24.146889	25	19	30
68	0	24.171163	25	19.25	30
69	0	24.265746	25	19.5	30
70	0	24.280974	25	19.5	30
71	0	24.27155	25	19.5	30
72	0	24.224658	25	19.5	30
73	0	24.22305	25	19.75	30
74	0	24.177484	25	19.5	30
75	0	24.014052	25	19	30
76	0	23.861533	25	19	30
77	0	23.673791	25	18	30
78	0	23.486388	25	17.75	30
79	0	23.319563	25	17.25	30
80	0	23.488291	25	17.5	30
81	0	23.579885	25	17.75	30
82	0	23.578635	25	18	30
83	0	23.534571	25	18	30
84	0	23.601866	25	18	30
85	0	23.56458	25	18	30
86	0	23.400967	25	17.5	30
87	0	23.224696	25	17	30
88	0	23.236174	25	17	30
89	0	23.246118	24.75	17	30
90	0	23.265	24.5	17.25	30
91	0	23.311711	24.5	17.25	30
92	0	23.351698	24.5	17.5	30
93	0	23.491281	24.75	17.5	30
94	0	23.474201	24.5	17.5	30
95	0	23.182678	24	17	30
96	0	23.195767	24	17	30
97	0	23.039102	23.5	16.75	30
98	0	22.934592	23.5	16.666667	30
99	0	22.834403	23.25	17	29.5
100	0	22.670818	23.25	16.666667	29.25
101	0	22.24968	22.5	16.25	29.25
102	0	21.765891	21.75	15.75	28
103	0	21.487053	21.25	15.5	27.5
104	0	21.047943	20.5	15.25	26.75
105	0	19.969933	19.5	14.25	25
106	0	19.055688	18.5	13.5	24.25
107	0	17.877283	17.5	12.75	22.75
108	0	10.247443	9.5	6	12.5