## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5381-Z0065-CTTGTGTCATGAGAT/441525-L5381-Z0065-CTTGTGTCATGAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5381-Z0065-CTTGTGTCATGAGAT/441525-L5381-Z0065-CTTGTGTCATGAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:20 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999078	999078	209	208912622	176121456	0.843039	146009851	0.698904	310403404	310403404

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2029354	26.239902	26.25	26.25	26.25
1	188494	34.423176	35	34.25	35
2	3988	34.659021	35	35	35
3	1077667	34.480202	35	34.25	35
4	8906	33.194173	34.25	32.5	35
5	9828	34.731317	35	35	35
6	2707558	32.882491	34.25	31.75	35
7	12156	32.457293	33.75	31	35
8	15062	33.267683	33.75	32.5	35
9	4741325	33.386194	34.25	32.5	35
10	6772	34.099437	35	34.25	35
11	14516	32.420101	33.75	31.75	35
12	5803460	32.969054	35	32.5	35
13	36397	32.914034	35	32.5	35
14	593586	33.003773	35	32.5	35
15	4357122	32.954717	35	32.5	35
16	603684	33.079607	35	32.5	35
17	5048062	32.99538	35	32.5	35
18	151	32.975195	35	32.5	35
19	5533078	32.964064	35	32.5	35
20	270099	32.934134	35	32.5	35
21	306391	32.914044	35	32.5	35
22	3227141	32.722564	35	32.5	35
23	0	32.553154	35	31.75	35
24	32531	32.274458	35	31.75	35
25	25530725	31.687625	34.25	31.5	35
26	0	31.510355	34.25	31.25	35
27	26	31.036042	34.25	30.5	35
28	201706	30.505089	34.25	29.5	35
29	542986	29.931322	33.75	28.5	35
30	5239791	29.376501	33.75	26.5	35
31	0	28.835511	33.5	24.75	35
32	8869455	28.270115	32.75	22.5	35
33	0	27.783844	32.5	21	35
34	0	27.400333	31.75	20	35
35	131900605	27.032169	31.25	19.5	35
36	0	26.87051	30.5	19.5	35
37	0	26.560669	29.75	19	35
38	0	26.312125	28.5	19	35
39	0	26.060519	27.5	18.75	35
40	0	25.765954	26.75	18.5	34.25
41	0	25.532931	26	18	34.25
42	0	25.273717	25.25	18	34.25
43	0	25.073795	25	17.75	34.25
44	0	24.883542	25	17.5	33.75
45	0	24.761618	25	17.25	33.75
46	0	24.651872	25	17.25	33.75
47	0	24.63361	25	17.25	33.75
48	0	24.597184	25	17.25	33.75
49	0	24.52639	25	17.25	33.75
50	0	24.457169	25	17.25	33.75
51	0	24.319337	25	17.25	33.5
52	0	24.251431	25	17.25	33.5
53	0	24.033081	24.5	17	33
54	0	23.837035	23.5	17	32.5
55	0	23.633131	23.5	17	32.5
56	0	23.649318	23.5	17	32.5
57	0	23.563131	23.5	17	31.75
58	0	23.592353	23.5	17.25	31.75
59	0	23.470903	23.5	17.25	31.75
60	0	23.369709	23.5	17.25	31
61	0	23.311241	23.5	17.5	30
62	0	23.303139	23.5	17.75	30
63	0	23.302741	23.5	17.75	30
64	0	23.357407	23.5	18.25	30
65	0	23.428021	24.25	18.25	30
66	0	23.492677	24.75	18.5	30
67	0	23.522099	25	18.5	30
68	0	23.596363	25	18.5	30
69	0	23.689543	25	18.75	30
70	0	23.729685	25	18.75	30
71	0	23.743031	25	19	30
72	0	23.738264	25	19	30
73	0	23.729227	25	19	30
74	0	23.721552	25	19	29.75
75	0	23.559847	25	18.75	29.25
76	0	23.380059	25	18.5	29.25
77	0	23.13966	25	17.5	29.75
78	0	22.853368	25	17	29.75
79	0	22.730892	24.5	16.5	30
80	0	22.850205	25	16.75	30
81	0	22.92976	25	17	30
82	0	23.050889	25	17.25	30
83	0	23.052871	25	17.25	30
84	0	23.190831	25	17.75	30
85	0	23.163094	24.75	17.5	30
86	0	22.930814	24.25	17	29.25
87	0	22.704695	24	16.666667	29.25
88	0	22.649848	23.5	16.5	29.25
89	0	22.529233	23.5	16.25	29.25
90	0	22.490978	23.5	16.5	29.25
91	0	22.547059	23.5	16.666667	29.25
92	0	22.714737	23.5	17	29.25
93	0	22.943127	23.75	17	29.75
94	0	22.894537	23.5	16.75	29.75
95	0	22.727876	23.5	16.75	29.25
96	0	22.692241	23.5	16.5	29.75
97	0	22.593409	23.25	16.5	29.75
98	0	22.53215	23	16.5	29.25
99	0	22.4582	22.75	16.25	29.25
100	0	22.19249	22.5	16	29.25
101	0	21.861913	21.75	16	28
102	0	21.23629	21	15.25	27.5
103	0	21.158102	20.75	15.25	27
104	0	20.830836	20.25	15	26.25
105	0	19.821711	19.5	14	25
106	0	18.624898	17.75	13.25	23.5
107	0	17.465999	17	12.25	22.5
108	0	9.835628	9.5	6	12.5