## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5380-Z0048-CTGCCAGACTGTGAT/441525-L5380-Z0048-CTGCCAGACTGTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5380-Z0048-CTGCCAGACTGTGAT/441525-L5380-Z0048-CTGCCAGACTGTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:23 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999244	999244	209	209192432	176297677	0.842754	145517828	0.695617	310272574	310272574

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2031182	26.225664	26.25	26.25	26.25
1	199313	34.50444	35	34.25	35
2	4234	34.562256	35	34.25	35
3	1166149	34.14172	35	34.25	35
4	9078	32.577507	32.5	31.75	34.25
5	10384	34.714343	35	35	35
6	2754377	33.116896	34.25	32.5	35
7	12640	32.504677	33.75	31	35
8	16003	33.204372	33.75	32.5	35
9	4771750	33.366055	34.25	32.5	35
10	7254	34.201864	35	34.25	35
11	15624	32.459095	33.75	31.75	35
12	5825049	32.988644	35	32.5	35
13	37770	32.903366	35	32.5	35
14	611100	32.97014	35	32.5	35
15	4343245	32.897588	35	32.5	35
16	613341	32.985303	35	32.5	35
17	4992495	32.902246	35	32.5	35
18	154	32.907255	35	32.5	35
19	5473613	32.885396	35	32.5	35
20	278799	32.841981	35	32.5	35
21	306304	32.776588	35	32.5	35
22	3295485	32.635558	35	31.75	35
23	0	32.409371	35	31.75	35
24	33191	32.066072	34.25	31.75	35
25	26114189	31.500812	34.25	31	35
26	0	31.12833	34.25	30.5	35
27	23	30.59252	34.25	29.75	35
28	203597	30.016838	33.75	28.5	35
29	548261	29.45642	33.75	26.75	35
30	5277013	28.911854	33.5	25	35
31	0	28.42328	33	23	35
32	9186342	27.917363	32.5	21.25	35
33	0	27.48369	31.75	20.25	35
34	0	27.177659	31.5	19.75	35
35	131054473	26.847346	30.5	19.5	35
36	0	26.693794	30	19.5	35
37	0	26.395719	29	19	35
38	0	26.161407	27.75	19	35
39	0	25.933466	27.5	18.75	34.25
40	0	25.659393	26.75	18.5	34.25
41	0	25.472185	26	18.25	34.25
42	0	25.277881	25.25	18	34.25
43	0	25.117121	25	17.75	34.25
44	0	24.980303	25	17.75	33.75
45	0	24.886478	25	17.5	33.75
46	0	24.797712	25	17.5	33.75
47	0	24.789863	25	17.5	33.75
48	0	24.803849	25	17.5	33.75
49	0	24.764944	25	17.5	33.75
50	0	24.706212	25	17.5	33.75
51	0	24.626895	25	17.5	33.75
52	0	24.595382	25	17.75	33.75
53	0	24.417626	25	17.5	33.5
54	0	24.262818	25	17.5	33.5
55	0	24.044666	25	17.25	33
56	0	24.095777	25	17.5	32.5
57	0	24.0134	25	17.5	32.5
58	0	24.041138	25	17.75	32.5
59	0	23.936122	24.75	17.5	32.5
60	0	23.861855	24.25	17.75	31.75
61	0	23.856698	24.5	17.75	31.75
62	0	23.867978	24.5	18	31.25
63	0	23.876824	25	18.5	31
64	0	23.960028	25	18.5	30
65	0	24.012233	25	18.75	30
66	0	24.071025	25	19	30
67	0	24.10513	25	19	30
68	0	24.165984	25	19	30
69	0	24.239223	25	19.25	30
70	0	24.26393	25	19.5	30
71	0	24.317212	25	19.5	30
72	0	24.358819	25	19.75	30
73	0	24.308701	25	19.75	30
74	0	24.298518	25	19.75	30
75	0	24.184239	25	19.5	30
76	0	24.063491	25	19	30
77	0	23.823628	25	18.5	30
78	0	23.639841	25	18	30
79	0	23.570668	25	17.5	30
80	0	23.638353	25	17.75	30
81	0	23.744244	25	18	30
82	0	23.79301	25	18.25	30
83	0	23.82918	25	18.5	30
84	0	23.837305	25	18.5	30
85	0	23.728042	25	18.25	30
86	0	23.505596	25	17.75	30
87	0	23.308367	25	17.25	30
88	0	23.331493	25	17.25	30
89	0	23.238772	24.75	17	30
90	0	23.358399	24.5	17.5	30
91	0	23.299873	24.5	17.5	30
92	0	23.358359	24.5	17.5	30
93	0	23.513833	24.75	17.75	30
94	0	23.485708	24.5	17.5	30
95	0	23.292702	24.25	17.25	30
96	0	23.072915	23.5	17	30
97	0	22.805497	23.5	16.5	30
98	0	22.809905	23.5	16.75	29.25
99	0	22.654888	23.25	16.666667	29.25
100	0	22.459463	22.75	16.25	29.25
101	0	21.956163	22	15.5	28.5
102	0	21.402757	21	15.5	27.5
103	0	21.333647	21	15.5	27.25
104	0	20.832769	20.5	15	26.75
105	0	19.892593	19.5	14.25	25
106	0	19.042395	18.5	13.5	24.25
107	0	17.841535	17.5	12.75	22.75
108	0	10.072173	9.5	6	12.5