## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5377-Z0056-CTTATGCTATCAGAT/441525-L5377-Z0056-CTTATGCTATCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5377-Z0056-CTTATGCTATCAGAT/441525-L5377-Z0056-CTTATGCTATCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:24 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000160	1000160	195	195468863	162850422	0.833127	134460400	0.687887	288115264	288115264

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2029831	26.240144	26.25	26.25	26.25
1	198263	34.402217	35	34.25	35
2	3017	34.602206	35	34.25	35
3	1066373	34.496829	35	34.25	35
4	7629	32.992277	33.5	31.75	34.25
5	8046	34.710245	35	35	35
6	2717972	32.705911	33.5	31.75	35
7	10988	32.38666	33.75	30.5	35
8	13127	33.318163	33.75	32.5	35
9	4770779	33.41706	34.25	32.5	35
10	6204	34.170094	35	34.25	35
11	13053	32.381363	33.75	31.75	35
12	5835739	32.914111	35	32.5	35
13	31938	32.855013	35	32.5	35
14	586256	32.944545	35	32.5	35
15	4350526	32.896983	35	32.5	35
16	580826	32.99776	35	32.5	35
17	5011527	32.92768	35	32.5	35
18	88	32.913189	35	32.5	35
19	5376259	32.876364	35	32.5	35
20	246733	32.815744	35	32.5	35
21	259147	32.736858	35	32.5	35
22	2929495	32.455781	35	31.75	35
23	0	32.122297	35	31.75	35
24	28503	31.61183	34.25	31	35
25	24276071	30.729493	34.25	30	35
26	0	30.328558	33.75	29.25	35
27	22	29.636546	33.75	27.5	35
28	175481	28.954024	33.75	25	35
29	474570	28.310669	33	22.75	35
30	4618462	27.758762	32.5	20.5	35
31	0	27.271615	31.75	19.5	35
32	8486754	26.783008	31	18.5	35
33	0	26.367874	30	18.25	35
34	0	26.043177	28.5	18	35
35	121355184	25.693731	27.25	17.75	35
36	0	25.547237	26	18	34.25
37	0	25.215817	25	17.75	34.25
38	0	24.997832	25	17.5	34.25
39	0	24.791326	25	17.5	33.75
40	0	24.538192	25	17	33.75
41	0	24.394708	25	17	33.75
42	0	24.219319	25	17	33.75
43	0	24.100135	25	16.75	33.75
44	0	23.985625	25	16.75	33.5
45	0	23.903811	24.5	16.5	33.5
46	0	23.828957	24.25	16.5	33.5
47	0	23.827433	24.25	16.5	33.5
48	0	23.843477	24.25	16.5	33.25
49	0	23.796105	24	16.5	33
50	0	23.749588	23.5	16.75	33
51	0	23.622572	23.5	16.666667	32.5
52	0	23.601283	23.5	16.75	32.5
53	0	23.422877	23.5	16.5	32.5
54	0	23.236707	23	16.5	31.75
55	0	23.029774	23	16.25	31.75
56	0	23.066627	23	16.5	31.75
57	0	22.974682	23	16.5	31.25
58	0	23.003457	23	16.75	31
59	0	22.899623	23	16.75	30
60	0	22.788542	23	16.75	30
61	0	22.792209	23	17	30
62	0	22.788071	23	17	29.75
63	0	22.780509	23.25	17.25	29.25
64	0	22.841002	23.5	17.75	29.25
65	0	22.953254	23.5	17.75	29.25
66	0	22.992748	23.5	17.75	29.25
67	0	23.054805	23.5	18	29.25
68	0	23.179637	24.25	18.25	29.75
69	0	23.296056	25	18.5	30
70	0	23.360967	25	18.5	30
71	0	23.393909	25	18.75	29.25
72	0	23.411731	25	18.75	29.25
73	0	23.466247	25	19	29.25
74	0	23.458805	25	19	29.25
75	0	23.297923	25	18.5	29.25
76	0	23.11065	25	18	29.25
77	0	22.910635	25	17	29.25
78	0	22.691281	24.5	16.666667	29.25
79	0	22.605848	24.5	16.25	29.5
80	0	22.80834	25	16.75	30
81	0	22.816238	25	16.75	30
82	0	22.979653	25	17	30
83	0	23.02088	25	17.25	30
84	0	23.062189	25	17.5	30
85	0	22.969386	24.75	17	29.25
86	0	22.835467	24.25	17	29.25
87	0	22.5562	23.75	16.5	29.25
88	0	22.553954	23.75	16.25	29.25
89	0	22.590381	23.75	16.5	29.25
90	0	22.628216	23.5	16.666667	29.25
91	0	22.568782	23.5	16.5	29.25
92	0	22.604479	23.5	16.5	29.25
93	0	23.050806	24.25	17	30
94	0	23.014399	23.5	17	30
95	0	23.016956	23.75	16.75	30
96	0	22.61632	23.5	16.25	29.25
97	0	22.534099	23.25	16.25	29.75
98	0	22.440776	23.25	16.25	29.25
99	0	22.581002	23	16.5	29.25
100	0	22.259865	22.5	16.25	29.25
101	0	21.725025	21.75	15.5	28.5
102	0	21.581427	21.25	15.5	27.75
103	0	21.248298	21	15.25	26.75
104	0	20.633065	20	15	26
105	0	19.622738	18.75	14	25
106	0	18.699511	18	13.25	23.5
107	0	17.595693	17	12.25	22.25
108	0	10.053583	9.5	6	12.5