## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5376-Z0051-CACTTGCAGATCGAT/441525-L5376-Z0051-CACTTGCAGATCGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5376-Z0051-CACTTGCAGATCGAT/441525-L5376-Z0051-CACTTGCAGATCGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:02 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999226	999226	195	195051294	161780419	0.829425	130772915	0.670454	285033374	285033374

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2029821	26.240915	26.25	26.25	26.25
1	204869	34.647934	35	35	35
2	3608	34.652292	35	34.25	35
3	1194575	33.604011	34.25	32.5	35
4	7017	31.554416	31.75	30	33.5
5	9390	34.714445	35	35	35
6	2774454	32.630843	33.5	31.75	35
7	11463	32.210631	32.5	30.25	35
8	15417	33.331422	33.75	32.5	35
9	4913463	32.877926	34.25	31.75	35
10	6993	33.637514	34.25	32.5	35
11	15165	32.148854	33.75	31.25	35
12	5965329	32.678218	35	31.75	35
13	36778	32.603086	34.25	31.75	35
14	590230	32.687872	35	31.75	35
15	4409379	32.616723	34.25	31.75	35
16	612777	32.7243	35	31.75	35
17	5022428	32.633878	35	31.75	35
18	91	32.620011	34.25	31.75	35
19	5447628	32.58064	34.25	31.75	35
20	285989	32.497002	34.25	31.75	35
21	267569	32.372353	34.25	31.75	35
22	3366328	32.102236	34.25	31.25	35
23	0	31.731593	34.25	31	35
24	32788	31.161907	33.75	30	35
25	26383427	30.358829	33.75	29.25	35
26	0	29.855196	33.75	27.75	35
27	14	29.187874	33.5	26.25	35
28	160986	28.531522	32.75	24.25	35
29	510403	27.931215	32.5	21.75	35
30	4798118	27.402141	31.75	20	35
31	0	26.97964	31.25	19.5	35
32	9270479	26.54739	30	18.75	35
33	0	26.196246	29.25	18.5	35
34	0	25.923558	27.5	18.25	34.25
35	116704318	25.603861	26.75	18	34.25
36	0	25.479061	26	18.25	34.25
37	0	25.183324	25	18	33.75
38	0	24.974957	25	18	33.75
39	0	24.758442	25	17.75	33.75
40	0	24.478802	25	17.5	33.5
41	0	24.281604	25	17.25	33.5
42	0	24.060302	25	17	33
43	0	23.909344	25	17	32.75
44	0	23.725657	24.25	16.75	32.5
45	0	23.607831	23.75	16.5	32.5
46	0	23.501383	23.5	16.5	32.5
47	0	23.473519	23.5	16.5	32.5
48	0	23.455189	23.5	16.5	32.5
49	0	23.389733	23.5	16.5	32
50	0	23.354469	23.5	16.5	31.75
51	0	23.265258	23.5	16.75	31.75
52	0	23.269956	23.5	16.75	31.75
53	0	23.094611	23	16.75	31.25
54	0	22.939969	23	16.5	31
55	0	22.750255	23	16.5	30
56	0	22.808898	23	16.666667	30
57	0	22.740808	23	16.75	30
58	0	22.827984	23	17	30
59	0	22.718208	23	16.75	30
60	0	22.676601	23	17	30
61	0	22.683612	23	17	29.25
62	0	22.722216	23	17.25	29.25
63	0	22.767554	23.5	17.5	29.25
64	0	22.856337	23.5	17.75	29
65	0	22.934204	23.5	17.75	29.25
66	0	23.046022	23.5	18	29.25
67	0	23.059191	24	18	29.25
68	0	23.149167	24.25	18	30
69	0	23.263958	25	18.25	30
70	0	23.360003	25	18.5	30
71	0	23.35523	25	18.5	30
72	0	23.326603	25	18.5	29.25
73	0	23.37049	25	18.5	29.25
74	0	23.389618	25	18.5	29.25
75	0	23.225099	25	18.25	29.25
76	0	23.063408	25	17.75	29.25
77	0	22.812443	24.5	17	29.25
78	0	22.622317	24.25	16.666667	29.25
79	0	22.504435	24	16.25	29.25
80	0	22.677638	24.25	16.5	29.25
81	0	22.795232	24.5	17	29.25
82	0	22.749873	24.5	17	29.25
83	0	22.793548	24.25	17	29.25
84	0	22.778809	24.25	17.25	29.25
85	0	22.678324	23.5	17	29.25
86	0	22.541486	23.5	16.5	29
87	0	22.268552	23.5	16.25	28.5
88	0	22.304165	23.5	16.25	28.75
89	0	22.290733	23	16.25	28.75
90	0	22.023421	22.75	16	28.5
91	0	22.094247	22.75	16.25	28.5
92	0	21.955117	22.75	16.25	28.5
93	0	22.314019	22.75	16.5	29
94	0	22.261957	22.75	16.5	28.5
95	0	21.836492	22	15.75	28
96	0	21.48332	21.75	15.5	27.75
97	0	21.711359	22	15.5	28
98	0	21.584111	21.75	15.5	27.5
99	0	21.528572	21.75	15.5	27.5
100	0	21.381166	21.75	15.5	27.5
101	0	21.04041	21	15.25	27.5
102	0	20.910083	20.5	15	26.75
103	0	20.556665	20	14.75	26.25
104	0	19.894998	19.25	14	25.25
105	0	18.834836	18	13.25	23.5
106	0	17.919297	17	12.5	22.75
107	0	16.88077	16.25	11.75	21.75
108	0	9.606513	9.333333	6	12.5