## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5371-Z0039-CACAACATATCAGAT/441525-L5371-Z0039-CACAACATATCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5371-Z0039-CACAACATATCAGAT/441525-L5371-Z0039-CACAACATATCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:56 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999444	999444	202	201983015	168770653	0.835569	138732593	0.686853	297511866	297511866

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2032482	26.241167	26.25	26.25	26.25
1	213520	34.274287	34.25	34.25	35
2	4384	34.652905	35	34.25	35
3	1295090	34.124404	35	34.25	35
4	8222	31.98953	32.5	30	34.25
5	10411	34.715765	35	35	35
6	2887759	33.11712	34.25	32.5	35
7	12020	32.550353	33.75	31	35
8	16827	33.328648	33.75	32.5	35
9	4869791	33.636745	34.25	33	35
10	7783	34.258113	35	34.25	35
11	16744	32.438747	33.75	31.75	35
12	5931496	32.716696	35	31.75	35
13	40518	32.583515	35	31.75	35
14	625734	32.654105	35	31.75	35
15	4388175	32.609433	35	31.75	35
16	618388	32.664964	35	31.75	35
17	4935183	32.559527	35	31.75	35
18	114	32.560265	35	31.75	35
19	5297721	32.539519	35	31.75	35
20	297900	32.481971	35	31.75	35
21	298722	32.371649	34.25	31.75	35
22	3342995	32.203011	34.25	31.75	35
23	0	31.922532	34.25	31	35
24	33912	31.491046	34.25	30.5	35
25	25332884	30.819606	33.75	29.75	35
26	0	30.44204	33.75	29.25	35
27	22	29.905171	33.75	28	35
28	194031	29.364254	33.75	26.75	35
29	537594	28.846059	33.25	25	35
30	5068142	28.358729	32.5	23	35
31	0	27.895152	32.5	21.5	35
32	9188679	27.418222	31.75	20.25	35
33	0	26.991309	31.25	19.5	35
34	0	26.657136	30	18.75	35
35	124475772	26.297486	29.25	18.5	35
36	0	26.127263	28.5	18.5	35
37	0	25.818154	27.5	18.25	34.25
38	0	25.585991	26.5	18	34.25
39	0	25.36725	25.5	17.75	34.25
40	0	25.090503	25	17.5	34.25
41	0	24.91771	25	17.5	33.75
42	0	24.715681	25	17.25	33.75
43	0	24.571283	25	17	33.75
44	0	24.427485	25	17	33.75
45	0	24.341382	25	17	33.75
46	0	24.275131	25	17	33.75
47	0	24.276087	25	17	33.75
48	0	24.289835	25	17	33.75
49	0	24.2382	25	17	33.75
50	0	24.193668	25	17	33.5
51	0	24.093252	25	17	33.5
52	0	24.076974	25	17	33.25
53	0	23.897596	24	17	32.5
54	0	23.714641	23.5	16.75	32.5
55	0	23.512475	23.5	16.666667	32.5
56	0	23.562422	23.5	17	32.5
57	0	23.493051	23.5	17	31.75
58	0	23.546601	23.5	17	31.75
59	0	23.448358	23.5	17	31.75
60	0	23.42128	23.5	17.25	31.5
61	0	23.400846	23.5	17.25	31
62	0	23.425702	23.5	17.5	30.25
63	0	23.459467	23.5	17.75	30
64	0	23.538871	24	18	30
65	0	23.586119	24.5	18.25	30
66	0	23.657248	25	18.5	30
67	0	23.748052	25	18.5	30
68	0	23.809445	25	18.5	30
69	0	23.912649	25	18.75	30
70	0	23.998668	25	19	30
71	0	24.067922	25	19.25	30
72	0	24.068073	25	19.25	30
73	0	24.140684	25	19.5	30
74	0	24.120833	25	19.5	30
75	0	24.04441	25	19	30
76	0	23.885184	25	18.75	30
77	0	23.664214	25	18	30
78	0	23.485881	25	17.75	30
79	0	23.394486	25	17.25	30
80	0	23.503147	25	17.5	30
81	0	23.643341	25	17.75	30
82	0	23.741982	25	18.25	30
83	0	23.665764	25	18.25	30
84	0	23.806252	25	18.5	30
85	0	23.853089	25	18.25	30
86	0	23.678457	25	18	30
87	0	23.408174	25	17.25	30
88	0	23.406027	25	17.5	30
89	0	23.370513	25	17.5	30
90	0	23.372349	24.75	17.75	30
91	0	23.396372	24.5	17.5	30
92	0	23.504179	25	17.75	30
93	0	23.602529	24.75	17.75	30
94	0	23.565107	24.5	17.75	30
95	0	23.441627	24.25	17.5	30
96	0	23.122174	23.5	17	30
97	0	23.045255	23.5	17	30
98	0	23.010627	23.5	17	30
99	0	22.720977	23	17	29.25
100	0	22.419767	22.75	16.5	29.25
101	0	22.179004	22.25	16	28.75
102	0	21.802784	21.75	15.75	27.75
103	0	21.463148	21.25	15.5	27.5
104	0	21.007812	20.75	15.25	26.75
105	0	20.191281	19.5	14.5	25.5
106	0	19.357521	18.75	13.75	25
107	0	17.92863	17.5	12.75	22.75
108	0	10.252501	9.5	6	12.5