## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5287-Z0032-CTCTGTATTGCAGAT/441525-L5287-Z0032-CTCTGTATTGCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5287-Z0032-CTCTGTATTGCAGAT/441525-L5287-Z0032-CTCTGTATTGCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:23 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
998320	998320	201	201097550	167963584	0.835234	138578038	0.689109	296835982	296835982

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2026721	26.241272	26.25	26.25	26.25
1	191096	34.249381	35	34.25	35
2	4354	34.549111	35	34.25	35
3	1049251	34.20453	35	34.25	35
4	8735	32.167875	32.5	31.75	34.25
5	9169	34.743713	35	35	35
6	2778822	33.146241	34.25	32.5	35
7	11028	32.389513	33.75	30.5	35
8	14129	33.18146	33.75	32.5	35
9	4843322	33.448357	34.25	32.5	35
10	6504	34.21005	35	34.25	35
11	13582	32.573079	33.75	31.75	35
12	5874832	33.056597	35	32.5	35
13	36913	33.005267	35	32.5	35
14	597150	33.123146	35	32.75	35
15	4403870	33.095914	35	32.5	35
16	602314	33.156137	35	33	35
17	5113159	33.085045	35	32.5	35
18	67	33.078359	35	32.5	35
19	5548948	33.066586	35	32.5	35
20	259640	33.012545	35	32.5	35
21	307617	32.914085	35	32.5	35
22	3056420	32.740963	35	32.5	35
23	0	32.451732	35	31.75	35
24	30816	32.011358	34.25	31.75	35
25	25008323	31.352472	34.25	31	35
26	0	30.83748	34.25	30	35
27	32	30.204518	33.75	29.25	35
28	205710	29.540651	33.75	27.25	35
29	516988	28.917882	33.75	25	35
30	4931962	28.341795	33	22.5	35
31	0	27.815667	32.5	21	35
32	8643573	27.300803	31.75	19.5	35
33	0	26.865241	31	19	35
34	0	26.541823	30	18.5	35
35	125002503	26.196611	29	18.25	35
36	0	26.037804	27.5	18.5	35
37	0	25.694903	26.75	18.25	34.25
38	0	25.4073	25	18	34.25
39	0	25.144904	25	17.75	34.25
40	0	24.81971	25	17.5	33.75
41	0	24.574899	25	17.25	33.75
42	0	24.311369	25	17	33.75
43	0	24.105221	25	16.75	33.5
44	0	23.920503	24.5	16.75	33.5
45	0	23.773753	24	16.5	33
46	0	23.657671	23.5	16.5	32.75
47	0	23.621156	23.5	16.5	32.5
48	0	23.575283	23.5	16.5	32.5
49	0	23.476588	23.5	16.5	32.5
50	0	23.388745	23.5	16.5	32
51	0	23.233586	23.5	16.5	31.75
52	0	23.177179	23.333333	16.5	31.75
53	0	22.984922	23	16.5	31.25
54	0	22.817328	23	16.5	31
55	0	22.646409	22.5	16.25	30.25
56	0	22.764906	23	16.5	30.25
57	0	22.753333	23	16.5	30
58	0	22.881166	23	16.75	30
59	0	22.869872	23	17	30
60	0	22.892518	23	17	30
61	0	22.98982	23.5	17	30
62	0	23.108249	23.5	17.5	30
63	0	23.266817	23.5	17.75	30
64	0	23.40128	23.5	18	30
65	0	23.607009	25	18.25	30
66	0	23.76257	25	18.5	30
67	0	23.900762	25	18.5	30
68	0	24.054099	25	18.75	30.5
69	0	24.208524	25	19	30.5
70	0	24.351792	25	19.5	31
71	0	24.483396	25	19.5	30.5
72	0	24.566348	25	19.75	30.5
73	0	24.691217	25	20	30.5
74	0	24.787352	25.25	20.25	31
75	0	24.739042	25.25	20	31
76	0	24.685237	25.25	19.75	31
77	0	24.652405	25.5	19.5	31
78	0	24.574863	25.75	19.25	31.25
79	0	24.44545	25.75	18.75	31.25
80	0	24.52156	26	19	31.25
81	0	24.675093	26	19.5	31.75
82	0	24.665245	26	19.5	31.25
83	0	24.629672	26	19.5	31.25
84	0	24.600693	25.75	19.5	31
85	0	24.597562	25.5	19.5	31
86	0	24.324909	25	19	30.5
87	0	24.121772	25	18.75	30.25
88	0	24.195591	25.25	18.75	30.5
89	0	24.196299	25	18.75	30.5
90	0	24.092	25	18.5	30.25
91	0	24.035865	25	18.5	30.25
92	0	24.03752	25	18.5	30.5
93	0	24.175821	25	18.75	31
94	0	24.0005	25	18.25	31
95	0	23.847072	25	18	30.5
96	0	23.778082	24.5	18	30.25
97	0	23.499682	24.25	17.25	30
98	0	23.458264	24.25	17.25	30
99	0	23.184491	23.5	17.25	30
100	0	23.02567	23.5	17	30
101	0	22.67689	22.75	16.5	29.25
102	0	22.195679	22.25	16	28.75
103	0	21.895646	21.75	15.75	28
104	0	21.524533	21	15.5	27.5
105	0	20.635095	20.25	14.75	26
106	0	19.535579	18.75	13.75	25
107	0	18.313554	18	13	23
108	0	10.479919	10	6	12.5