## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5286-Z0030-CATGTCTAAGATGAT/441525-L5286-Z0030-CATGTCTAAGATGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5286-Z0030-CATGTCTAAGATGAT/441525-L5286-Z0030-CATGTCTAAGATGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:02 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999489	999489	259	259821134	226614101	0.872193	190470353	0.733083	394662071	394662071

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2039419	26.240129	26.25	26.25	26.25
1	185665	34.650621	35	35	35
2	9388	34.67667	35	35	35
3	1363922	34.566274	35	35	35
4	11971	33.280237	34.25	32.5	35
5	15583	34.773033	35	35	35
6	2797831	33.477645	35	32.5	35
7	14592	33.375597	35	32.5	35
8	23496	33.297929	33.75	32.5	35
9	4714708	33.627564	34.25	33.5	35
10	10194	34.254134	35	34.25	35
11	22356	32.787083	33.75	31.75	35
12	5747336	33.06548	35	32.5	35
13	59342	32.975198	35	32.5	35
14	679536	33.058434	35	32.5	35
15	4335990	32.998822	35	32.5	35
16	691344	33.070487	35	32.5	35
17	4913303	32.97354	35	32.5	35
18	134	32.986795	35	32.5	35
19	5570923	32.981988	35	32.5	35
20	382000	32.964876	35	32.5	35
21	506315	32.959405	35	32.5	35
22	4409876	32.9074	35	32.5	35
23	0	32.846302	35	32.5	35
24	49740	32.73704	35	32.5	35
25	29666765	32.574472	35	32	35
26	0	32.510793	35	31.75	35
27	37	32.401674	35	31.75	35
28	305088	32.24745	34.25	31.75	35
29	823927	32.084175	34.25	31.75	35
30	7888205	31.914818	34.25	31.75	35
31	0	31.708908	34.25	31.5	35
32	11126597	31.476755	34.25	31.25	35
33	0	31.254005	34.25	31	35
34	0	31.084489	34.25	30.5	35
35	171455551	30.87775	34.25	30	35
36	0	30.758857	34.25	30	35
37	0	30.532384	33.75	29.25	35
38	0	30.32378	33.75	28.5	35
39	0	30.092545	33.75	27.75	35
40	0	29.816694	33.75	26.75	35
41	0	29.600989	33.75	26	35
42	0	29.332119	33.5	25	35
43	0	29.129592	33.5	25	35
44	0	28.89849	33	25	35
45	0	28.726317	33	24	35
46	0	28.544333	32.5	23.5	35
47	0	28.410802	32.5	23.5	35
48	0	28.277621	32.5	23	35
49	0	28.115084	32.25	22.75	35
50	0	27.954637	31.75	22.5	35
51	0	27.721327	31.75	22	35
52	0	27.578606	31.5	21.5	35
53	0	27.282075	31	21	35
54	0	27.023189	30.25	20.5	35
55	0	26.695184	30	20	35
56	0	26.605798	29.75	20	34.25
57	0	26.407232	29.25	19.75	34.25
58	0	26.269873	28.5	19.75	34.25
59	0	26.0162	27.5	19.5	34.25
60	0	25.806767	26.75	19.5	33.75
61	0	25.671788	26	19.5	33.75
62	0	25.567495	25.5	19.5	33.5
63	0	25.476167	25	20	33
64	0	25.383901	25	20	32.5
65	0	25.356732	25	20.25	32.5
66	0	25.333192	25	20.25	31.75
67	0	25.249489	25	20.25	31.75
68	0	25.194969	25	20.25	31.75
69	0	25.193031	25.25	20.5	31.75
70	0	25.179223	25.5	20.5	31.75
71	0	25.137129	25.5	20.75	31.25
72	0	25.06525	25.5	20.5	31
73	0	25.004108	25.25	20.75	30.5
74	0	24.952045	25	20.5	30.25
75	0	24.79445	25	20.25	30
76	0	24.64333	25	20	30
77	0	24.454745	25	19.5	30.25
78	0	24.262443	25	19	30.25
79	0	24.12638	25	18.5	30.5
80	0	24.138853	25	18.75	30.25
81	0	24.207143	25.25	18.75	30.5
82	0	24.218858	25.25	19	30.5
83	0	24.186342	25.25	19	30.25
84	0	24.201743	25	19	30
85	0	24.163802	25	18.75	30
86	0	23.945196	25	18.5	30
87	0	23.762196	25	18	30
88	0	23.708112	25	18	30
89	0	23.672699	25	17.75	30
90	0	23.669676	25	18	30
91	0	23.628061	25	18	30
92	0	23.677213	25	18	30
93	0	23.775731	25	18	30
94	0	23.633188	24.5	17.75	30
95	0	23.492332	24.25	17.5	30
96	0	23.298771	24	17.25	30
97	0	23.125432	23.5	17	30
98	0	22.938201	23.5	17	29.75
99	0	22.753335	23.25	16.75	29.25
100	0	22.539481	22.75	16.5	29.25
101	0	22.198481	22.5	16.25	28.5
102	0	21.726762	21.75	15.75	27.75
103	0	21.470258	21.25	15.5	27.5
104	0	21.098948	20.75	15.5	26.75
105	0	20.18991	19.75	14.5	25.25
106	0	19.323691	18.75	13.75	24.75
107	0	18.09936	17.75	13	23
108	0	10.556683	10	6	13