## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5283-Z0027-CACTGTCAGCCAGAT/441525-L5283-Z0027-CACTGTCAGCCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5283-Z0027-CACTGTCAGCCAGAT/441525-L5283-Z0027-CACTGTCAGCCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:22 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1001067	1001067	253	254202158	221340426	0.870726	186277868	0.732794	385922017	385922017

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2041447	26.238191	26.25	26.25	26.25
1	179671	34.555485	35	34.25	35
2	9780	34.636402	35	34.25	35
3	1297835	34.348635	35	34.25	35
4	13190	33.010696	34.25	31.75	35
5	15808	34.748716	35	35	35
6	2782460	33.665222	35	32.5	35
7	14816	33.294382	35	32.5	35
8	22404	33.168801	33.75	32.5	35
9	4672921	33.752951	34.25	33.5	35
10	9711	34.331463	35	34.25	35
11	21144	32.868755	33.75	32.5	35
12	5697749	33.18152	35	32.75	35
13	54933	33.067239	35	32.5	35
14	658488	33.143431	35	32.5	35
15	4282577	33.103592	35	32.5	35
16	674825	33.161695	35	32.5	35
17	4888053	33.063151	35	32.5	35
18	173	33.074433	35	32.5	35
19	5523747	33.086424	35	32.5	35
20	348660	33.060634	35	32.5	35
21	480350	33.042633	35	32.5	35
22	4203166	32.988105	35	32.5	35
23	0	32.893402	35	32.5	35
24	46042	32.736117	35	32.5	35
25	28910161	32.494904	35	31.75	35
26	0	32.344807	35	31.75	35
27	42	32.111047	34.25	31.75	35
28	296467	31.847176	34.25	31.75	35
29	777670	31.587703	34.25	31.5	35
30	7617537	31.32722	34.25	31	35
31	0	31.058268	34.25	30.5	35
32	10807487	30.770774	34.25	30	35
33	0	30.513509	33.75	29.25	35
34	0	30.329882	33.75	28.75	35
35	167852844	30.126843	33.75	28	35
36	0	30.037638	33.75	27.5	35
37	0	29.816734	33.75	26.5	35
38	0	29.629199	33.75	25.25	35
39	0	29.43635	33.75	25	35
40	0	29.214026	33.5	25	35
41	0	29.061023	33.5	25	35
42	0	28.870894	33	24.5	35
43	0	28.717724	33	23.75	35
44	0	28.563791	32.5	23.5	35
45	0	28.477576	32.5	23.5	35
46	0	28.353186	32.5	23	35
47	0	28.29598	32.5	23	35
48	0	28.224501	32.5	23	35
49	0	28.113792	32.5	22.75	35
50	0	27.988857	31.75	22.5	35
51	0	27.791305	31.75	22	35
52	0	27.679874	31.75	21.75	35
53	0	27.407173	31.25	21	35
54	0	27.166338	31	20.75	35
55	0	26.866179	30	20.25	35
56	0	26.794502	30	20.25	35
57	0	26.625841	29.75	20	35
58	0	26.498131	29.25	20	34.25
59	0	26.276028	28.5	19.75	34.25
60	0	26.078152	27.5	19.5	34.25
61	0	25.950057	27.25	19.75	33.75
62	0	25.829463	26.25	19.75	33.75
63	0	25.732682	26	20	33.75
64	0	25.681662	25.75	20.25	33
65	0	25.64843	25.5	20.5	32.5
66	0	25.625276	26	20.5	32.5
67	0	25.571226	26	20.5	32.5
68	0	25.535285	26	20.5	32.5
69	0	25.548698	26	21	32.5
70	0	25.555987	26	21	31.75
71	0	25.545038	26	21	31.75
72	0	25.496003	26	21	31.75
73	0	25.476384	26	21.25	31.75
74	0	25.442128	26	21	31.75
75	0	25.315497	26	21	31.75
76	0	25.177412	26	20.5	31.75
77	0	24.999745	26	20.25	31.75
78	0	24.840787	26	19.75	31.75
79	0	24.713333	26	19.5	31.75
80	0	24.709901	26	19.5	31.5
81	0	24.7497	26	19.5	31.75
82	0	24.731618	26	19.5	31.75
83	0	24.707057	26	19.5	31.25
84	0	24.70976	26	19.5	31.25
85	0	24.601322	25.5	19.5	31
86	0	24.419957	25.25	19	31
87	0	24.240349	25.25	18.75	31
88	0	24.196392	25.25	18.5	31
89	0	24.143474	25.25	18.5	31
90	0	24.135088	25	18.5	31
91	0	24.078136	25	18.5	30.5
92	0	24.036893	25	18.5	30.5
93	0	24.162722	25	18.5	31
94	0	24.071119	25	18.5	31
95	0	23.835425	25	17.75	30.5
96	0	23.632776	24.5	17.75	30
97	0	23.462382	24.25	17.25	30
98	0	23.339011	24.25	17.25	30
99	0	23.140686	23.5	17	30
100	0	22.913337	23.5	17	29.25
101	0	22.61262	22.75	16.5	29.25
102	0	22.112846	22	16.25	28.5
103	0	21.86508	21.75	16	27.75
104	0	21.445181	21	15.5	27.25
105	0	20.575161	20.25	14.75	26
106	0	19.697188	19.5	14	25
107	0	18.448145	18	13	23.5
108	0	10.760413	10.333333	6	13.666667