## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5272-Z0011-CACGCACTGCCAGAT/441525-L5272-Z0011-CACGCACTGCCAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5272-Z0011-CACGCACTGCCAGAT/441525-L5272-Z0011-CACGCACTGCCAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:14 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1001158	1001158	216	216690670	182657562	0.842942	149993807	0.692202	320923354	320923354

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2037001	26.217203	26.25	26.25	26.25
1	202000	34.340424	35	34.25	35
2	6091	34.291453	35	34.25	35
3	1265755	34.082825	35	34.25	35
4	10689	32.425738	32.5	31.75	34.25
5	12112	34.728555	35	35	35
6	2893043	32.939785	34.25	31.75	35
7	13440	32.640163	33.75	31	35
8	18025	33.290842	33.75	32.5	35
9	4927775	33.516745	34.25	32.5	35
10	8297	34.162238	35	34.25	35
11	17390	32.511117	33.75	31.75	35
12	6020651	32.863551	35	32.5	35
13	44713	32.770025	35	31.75	35
14	635133	32.846509	35	32.5	35
15	4491423	32.794209	35	32.25	35
16	641073	32.874375	35	32.5	35
17	5132954	32.780344	35	32.25	35
18	119	32.791504	35	32.25	35
19	5655424	32.78535	35	32.5	35
20	303794	32.731818	35	31.75	35
21	357094	32.666711	35	31.75	35
22	3584308	32.513767	35	31.75	35
23	0	32.273	34.25	31.75	35
24	36648	31.866732	34.25	31.25	35
25	27559967	31.297574	34.25	30.5	35
26	0	30.849575	34.25	30	35
27	30	30.315782	33.75	29.25	35
28	222605	29.766525	33.75	27.75	35
29	599309	29.270168	33.75	26	35
30	5746545	28.821819	33.5	24.5	35
31	0	28.442315	33	23	35
32	9635301	28.049879	32.5	21.75	35
33	0	27.724174	31.75	21	35
34	0	27.486056	31.75	20.5	35
35	134611961	27.205997	31	20.25	35
36	0	27.071474	30.5	20.25	35
37	0	26.771771	30	19.75	35
38	0	26.555445	29.25	19.5	35
39	0	26.347413	28.75	19.25	35
40	0	26.081176	28	18.75	34.25
41	0	25.93028	27.5	18.5	34.25
42	0	25.759768	27.5	18.5	34.25
43	0	25.636172	27.5	18.25	34.25
44	0	25.526463	27	18.25	34.25
45	0	25.461607	26.75	18	34.25
46	0	25.381147	26.75	18	34.25
47	0	25.366591	26.25	18	34.25
48	0	25.357624	26	18	34.25
49	0	25.289052	26	18	34.25
50	0	25.219423	25.25	18.25	33.75
51	0	25.092462	25	18	33.75
52	0	25.045099	25	18.25	33.75
53	0	24.856946	25	18	33.75
54	0	24.689126	25	17.75	33.75
55	0	24.469222	25	17.75	33.5
56	0	24.51146	25	17.75	33.5
57	0	24.473106	25	18	33
58	0	24.49259	25	18	33
59	0	24.417752	25	18.25	32.5
60	0	24.355126	25	18.25	32.5
61	0	24.388505	25	18.5	32.5
62	0	24.412379	25	18.75	31.75
63	0	24.443612	25	19	31.75
64	0	24.54493	25	19.25	31.75
65	0	24.59349	25	19.5	31.75
66	0	24.66727	25	19.5	31.75
67	0	24.724504	25	19.5	31.75
68	0	24.780879	25	19.75	31.75
69	0	24.844412	25	19.75	31.75
70	0	24.900112	25	20	31.75
71	0	24.937147	25.5	20.25	31.25
72	0	24.963081	25.5	20.25	31.25
73	0	24.994877	25.5	20.5	31
74	0	25.000435	25.5	20.5	31
75	0	24.902947	25.5	20.25	31
76	0	24.818604	25.5	20	31
77	0	24.639726	25.5	19.5	31
78	0	24.416544	25.5	19	31
79	0	24.39622	25.5	18.75	31.25
80	0	24.451462	26	19	31
81	0	24.488266	26	19.25	31.25
82	0	24.509395	25.5	19.25	31
83	0	24.480349	25.5	19.5	31
84	0	24.542012	25.5	19.5	31
85	0	24.383637	25.25	19	31
86	0	24.285614	25.25	18.75	30.5
87	0	24.070232	25	18.5	30.5
88	0	23.965415	25	18.5	30.25
89	0	24.007352	25	18.25	30.5
90	0	23.892612	25	18.25	30
91	0	23.944778	25	18.25	30
92	0	23.867829	25	18.5	30
93	0	23.90674	25	18.25	30
94	0	23.765644	24.75	18	30
95	0	23.572577	24.5	17.5	30
96	0	23.370818	24.25	17.25	30
97	0	23.298437	24	17.25	30
98	0	23.121434	23.5	17	30
99	0	22.919454	23.5	17	29.25
100	0	22.748476	23	16.75	29.25
101	0	22.33822	22.75	16.25	29.25
102	0	21.845891	22	15.75	28
103	0	21.693693	21.5	16	27.5
104	0	21.183923	21	15.5	26.75
105	0	20.220693	19.75	14.5	25.25
106	0	19.26886	18.75	13.75	24.5
107	0	18.141868	18	13	23
108	0	10.448399	10	6	12.666667