## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5271-Z0010-CACTGCACGAATGAT/441525-L5271-Z0010-CACTGCACGAATGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5271-Z0010-CACTGCACGAATGAT/441525-L5271-Z0010-CACTGCACGAATGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:52:19 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
1000323	1000323	211	211257510	177316069	0.839336	145419278	0.688351	311827811	311827811

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2036039	26.235223	26.25	26.25	26.25
1	212414	34.630052	35	35	35
2	5632	34.524276	35	34.25	35
3	1309023	33.915552	34.25	32.5	35
4	10139	32.265429	32.5	31.75	34.25
5	11690	34.796616	35	35	35
6	2929802	33.023408	34.25	32.5	35
7	12998	32.538267	33.75	31	35
8	18119	33.22282	33.75	32.5	35
9	4933782	33.336409	34.25	32.5	35
10	8233	34.14344	35	34.25	35
11	17606	32.344146	33.75	31.75	35
12	6009462	32.661474	35	31.75	35
13	44655	32.563851	34.25	31.75	35
14	638769	32.671774	35	31.75	35
15	4478098	32.600705	34.25	31.75	35
16	642135	32.685099	35	31.75	35
17	5078074	32.597372	35	31.75	35
18	104	32.616021	35	31.75	35
19	5544667	32.608779	35	31.75	35
20	320210	32.553133	35	31.75	35
21	344660	32.4988	34.25	31.75	35
22	3595913	32.362376	34.25	31.75	35
23	0	32.158864	34.25	31.5	35
24	37217	31.810557	34.25	31	35
25	26778696	31.267714	34.25	30.25	35
26	0	30.887198	33.75	30	35
27	30	30.400071	33.75	29.25	35
28	219123	29.872427	33.75	28	35
29	600942	29.375005	33.75	26.75	35
30	5627732	28.889858	33.5	25	35
31	0	28.430135	32.5	23.5	35
32	9720318	27.948416	32.5	21.75	35
33	0	27.545112	31.75	20.5	35
34	0	27.229861	31.5	20	35
35	130071228	26.891559	30.75	19.5	35
36	0	26.732047	30	19.5	35
37	0	26.427648	29.25	19	35
38	0	26.177712	28.5	18.75	35
39	0	25.928618	27.5	18.5	34.25
40	0	25.628958	27	18.25	34.25
41	0	25.436234	26.25	18	34.25
42	0	25.196721	25.5	17.75	34.25
43	0	25.027831	25	17.5	34.25
44	0	24.874728	25	17.5	33.75
45	0	24.781325	25	17.25	33.75
46	0	24.687667	25	17.25	33.75
47	0	24.669475	25	17.25	33.75
48	0	24.67443	25	17.25	33.75
49	0	24.622195	25	17.5	33.75
50	0	24.582554	25	17.5	33.75
51	0	24.458435	25	17.5	33.75
52	0	24.431752	25	17.5	33.5
53	0	24.259917	25	17.5	33
54	0	24.088433	25	17.25	33
55	0	23.902723	24.5	17	32.5
56	0	23.985118	25	17.5	32.5
57	0	23.958916	24.5	17.5	32.5
58	0	24.030253	25	17.75	32.5
59	0	23.98941	25	17.75	32
60	0	23.953912	25	17.75	31.75
61	0	24.020248	25	18	31.75
62	0	24.073649	25	18.5	31.75
63	0	24.119009	25	18.5	31.25
64	0	24.195326	25	19	31
65	0	24.268283	25	19	31
66	0	24.346262	25	19	31
67	0	24.399444	25	19.25	31
68	0	24.466144	25	19.25	31.25
69	0	24.57998	25	19.5	31.25
70	0	24.668385	25	19.75	31
71	0	24.706421	25	20	31
72	0	24.737035	25	20	31
73	0	24.763865	25	20.25	30.5
74	0	24.7855	25	20.25	30.25
75	0	24.665174	25	20	30.25
76	0	24.574609	25	19.5	30.5
77	0	24.399408	25	19	30.5
78	0	24.150271	25	18.5	30.75
79	0	24.144347	25	18.5	31
80	0	24.20978	25.25	18.75	31
81	0	24.279354	25.25	18.75	31
82	0	24.324119	25.5	19	31
83	0	24.283121	25.25	19	31
84	0	24.338208	25.25	19	31
85	0	24.264719	25	19	30.5
86	0	24.119888	25	18.5	30.5
87	0	23.956507	25	18.25	30.25
88	0	23.932865	25	18.25	30.25
89	0	23.9681	25	18.25	30.25
90	0	23.859385	25	18.25	30
91	0	23.880237	25	18.25	30
92	0	23.803141	25	18.25	30
93	0	23.973764	25	18.25	30.5
94	0	23.834712	25	18	30.25
95	0	23.691904	24.5	17.75	30
96	0	23.469894	24.25	17.5	30
97	0	23.343873	24	17.25	30
98	0	23.211831	23.75	17	30
99	0	22.970544	23.5	17	29.75
100	0	22.821445	23.5	17	29.25
101	0	22.396556	22.75	16.25	29.25
102	0	22.088109	22	16	28.5
103	0	21.780762	21.75	16	27.5
104	0	21.200422	20.75	15.25	27
105	0	20.312224	20	14.5	25.5
106	0	19.403566	18.75	14	25
107	0	18.08917	17.75	13	23
108	0	10.325787	9.75	6	12.5