## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5268-Z0005-CATGTATCCTCTGAT/441525-L5268-Z0005-CATGTATCCTCTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5268-Z0005-CATGTATCCTCTGAT/441525-L5268-Z0005-CATGTATCCTCTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:52:32 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
998880	998880	215	215456636	181340631	0.841657	149507436	0.69391	319102705	319102705

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2032202	26.240483	26.25	26.25	26.25
1	206664	34.435071	35	34.25	35
2	5785	34.628744	35	34.25	35
3	1277959	34.397715	35	34.25	35
4	10108	32.707178	32.5	31.75	34.25
5	11812	34.774513	35	35	35
6	2904122	33.304164	34.25	32.5	35
7	13275	32.68228	33.75	31	35
8	17926	33.387915	33.75	32.5	35
9	4939711	33.529885	34.25	32.5	35
10	8068	34.24277	35	34.25	35
11	17461	32.433575	33.75	31.75	35
12	6042325	32.812574	35	32.5	35
13	44147	32.717014	35	31.75	35
14	645087	32.810521	35	32.5	35
15	4520170	32.756975	35	32	35
16	645560	32.822946	35	32.5	35
17	5143900	32.739041	35	31.75	35
18	146	32.740119	35	31.75	35
19	5629577	32.726278	35	31.75	35
20	308885	32.68714	35	31.75	35
21	348979	32.615762	35	31.75	35
22	3550359	32.467368	35	31.75	35
23	0	32.248109	34.25	31.75	35
24	36533	31.890255	34.25	31.25	35
25	26771269	31.335276	34.25	30.5	35
26	0	30.947989	34.25	30	35
27	21	30.464	33.75	29.25	35
28	220373	29.960662	33.75	28.5	35
29	596776	29.485544	33.75	26.75	35
30	5662746	29.044892	33.5	25	35
31	0	28.627041	33	23.75	35
32	9613621	28.207199	32.5	22.5	35
33	0	27.825507	32.5	21.25	35
34	0	27.540164	31.75	20.5	35
35	134231069	27.23301	31.25	20.25	35
36	0	27.079802	31	20	35
37	0	26.779636	30	19.5	35
38	0	26.540854	29.25	19.5	35
39	0	26.31769	28.75	19	35
40	0	26.035722	27.75	18.75	34.25
41	0	25.850636	27.5	18.5	34.25
42	0	25.623526	27.25	18.25	34.25
43	0	25.458319	26.75	18	34.25
44	0	25.308647	26	17.75	34.25
45	0	25.211291	26	17.75	34.25
46	0	25.115227	25.5	17.75	34.25
47	0	25.102585	25.25	17.75	33.75
48	0	25.070908	25	17.75	33.75
49	0	24.984121	25	17.75	33.75
50	0	24.922331	25	17.75	33.75
51	0	24.77905	25	17.75	33.75
52	0	24.73381	25	17.75	33.75
53	0	24.53229	25	17.75	33.5
54	0	24.365793	25	17.5	33.5
55	0	24.178715	25	17.25	33
56	0	24.229033	25	17.5	33
57	0	24.185813	25	17.75	32.5
58	0	24.259358	25	17.75	32.5
59	0	24.181947	25	17.75	32.5
60	0	24.145121	25	18	32.5
61	0	24.167852	25	18	31.75
62	0	24.213114	25	18.5	31.75
63	0	24.243073	25	18.5	31.75
64	0	24.31164	25	19	31.5
65	0	24.445189	25	19	31.75
66	0	24.501506	25	19.25	31.75
67	0	24.52801	25	19.5	31.25
68	0	24.593646	25	19.5	31.75
69	0	24.707906	25	19.75	31.75
70	0	24.766283	25	20	31.5
71	0	24.846538	25	20.25	31
72	0	24.876025	25	20.25	31
73	0	24.862473	25	20.5	30.75
74	0	24.900575	25.25	20.25	30.5
75	0	24.805155	25	20	30.5
76	0	24.702279	25.25	20	31
77	0	24.507229	25.25	19.25	31
78	0	24.343267	25.25	19	31
79	0	24.278409	25.25	18.75	31
80	0	24.347405	25.5	19	31
81	0	24.376705	25.5	19	31
82	0	24.398625	25.5	19.25	31
83	0	24.417245	25.5	19.25	31
84	0	24.370437	25.25	19.25	30.5
85	0	24.298035	25	19	30.5
86	0	24.16369	25	18.75	30
87	0	23.95124	25	18.25	30
88	0	23.973732	25	18.5	30
89	0	23.89484	25	18.25	30
90	0	23.788349	25	18	30
91	0	23.8177	25	18.25	30
92	0	23.766626	25	18	30
93	0	23.915068	25	18.25	30
94	0	23.771638	25	18	30
95	0	23.544247	24.5	17.5	30
96	0	23.282372	24	17.25	30
97	0	23.137933	23.5	17	30
98	0	23.081026	23.5	17	30
99	0	22.970515	23.5	17	29.25
100	0	22.710516	23	16.75	29.25
101	0	22.231692	22.5	16.25	28.5
102	0	21.86922	22	16	27.75
103	0	21.505585	21.25	15.75	27.5
104	0	20.983652	20.75	15.5	26.75
105	0	20.159584	19.75	14.5	25.25
106	0	19.236442	18.75	13.75	24.5
107	0	17.995877	17.75	12.75	23
108	0	10.383557	10	6	12.666667