## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5266-Z0001-CAGCTCGAATGCGAT/441525-L5266-Z0001-CAGCTCGAATGCGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5266-Z0001-CAGCTCGAATGCGAT/441525-L5266-Z0001-CAGCTCGAATGCGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:41 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
998951	998951	221	221439596	187423646	0.846387	154521789	0.697806	329125511	329125511

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2032886	26.231296	26.25	26.25	26.25
1	203384	34.650348	35	35	35
2	6241	34.540498	35	34.25	35
3	1269616	34.054147	35	34.25	35
4	10540	31.966689	32.5	30	34.25
5	12205	34.74917	35	35	35
6	2844886	33.088321	34.25	32.5	35
7	13501	32.701957	34.25	31	35
8	18294	33.401833	33.75	32.5	35
9	4911938	33.572794	34.25	32.5	35
10	8417	34.249666	35	34.25	35
11	18212	32.571992	33.75	31.75	35
12	6009757	32.869953	35	32.5	35
13	46620	32.782959	35	32.25	35
14	639438	32.861035	35	32.5	35
15	4494089	32.823329	35	32.5	35
16	644931	32.908995	35	32.5	35
17	5133965	32.822633	35	32.5	35
18	110	32.829251	35	32.5	35
19	5696920	32.82882	35	32.5	35
20	317488	32.779184	35	32.5	35
21	375205	32.722948	35	32	35
22	3619951	32.596907	35	31.75	35
23	0	32.407003	35	31.75	35
24	38663	32.107273	34.25	31.75	35
25	27674064	31.628599	34.25	31	35
26	0	31.305435	34.25	30.75	35
27	28	30.863619	34.25	30	35
28	241307	30.409773	33.75	29.25	35
29	635151	29.9625	33.75	28.5	35
30	5885779	29.540141	33.75	26.75	35
31	0	29.13592	33.75	25	35
32	9668036	28.728324	33.25	24.25	35
33	0	28.37265	32.5	23	35
34	0	28.102047	32.5	22	35
35	138967974	27.797988	31.75	21.5	35
36	0	27.643481	31.75	21	35
37	0	27.353856	31.25	20.5	35
38	0	27.088107	30.5	20.25	35
39	0	26.840743	30	20	35
40	0	26.553925	29.75	19.5	35
41	0	26.359447	29.25	19	35
42	0	26.120501	28.5	18.75	34.25
43	0	25.93015	27.75	18.5	34.25
44	0	25.772554	27.5	18.5	34.25
45	0	25.675542	27.5	18.5	34.25
46	0	25.570217	27.5	18.25	34.25
47	0	25.525769	26.75	18.25	34.25
48	0	25.481388	26.75	18.25	34.25
49	0	25.397486	26	18.25	34.25
50	0	25.32125	25.75	18.5	33.75
51	0	25.156122	25	18.25	33.75
52	0	25.097402	25	18.25	33.75
53	0	24.886897	25	18	33.75
54	0	24.695775	25	17.75	33.75
55	0	24.49048	25	17.75	33.5
56	0	24.536602	25	18	33.5
57	0	24.46427	25	18	33
58	0	24.495828	25	18	33
59	0	24.398902	25	18	32.5
60	0	24.342453	25	18.25	32.5
61	0	24.345578	25	18.5	32.5
62	0	24.395981	25	18.5	31.75
63	0	24.440859	25	19	31.75
64	0	24.476602	25	19	31.75
65	0	24.545557	25	19.25	31.75
66	0	24.603624	25	19.5	31.75
67	0	24.658317	25	19.5	31.75
68	0	24.699824	25	19.5	31.75
69	0	24.784544	25	19.75	31.75
70	0	24.829862	25	20	31.75
71	0	24.884196	25.25	20.25	31.25
72	0	24.905362	25.25	20.25	31.25
73	0	24.957374	25.5	20.5	31
74	0	24.991138	25.5	20.5	31
75	0	24.882138	25.5	20.25	31
76	0	24.777795	25.5	20	31
77	0	24.595409	25.5	19.5	31
78	0	24.431756	25.5	19	31
79	0	24.280566	25.5	18.5	31
80	0	24.366535	25.5	19	31
81	0	24.443668	25.75	19	31.25
82	0	24.495222	25.5	19	31.25
83	0	24.495035	25.5	19.25	31
84	0	24.514666	25.5	19.5	31
85	0	24.443409	25.25	19.25	31
86	0	24.229519	25	18.75	31
87	0	24.019518	25	18.5	30.5
88	0	23.942265	25	18.25	30.5
89	0	23.973471	25	18.25	30.5
90	0	23.903502	25	18.25	30
91	0	23.927555	25	18.5	30
92	0	23.846293	25	18.25	30
93	0	23.994854	25	18.5	30.25
94	0	23.757846	24.75	18	30
95	0	23.500952	24.25	17.5	30
96	0	23.366618	24.25	17.25	30
97	0	23.241314	23.75	17.25	30
98	0	23.013263	23.5	17	29.75
99	0	22.841551	23.5	17	29.25
100	0	22.534622	22.75	16.5	29.25
101	0	22.207535	22.5	16.25	28.5
102	0	21.770867	21.75	15.75	27.75
103	0	21.498197	21.25	15.75	27.5
104	0	20.968465	20.5	15.25	26.75
105	0	20.050003	19.75	14.5	25
106	0	19.194366	18.75	13.75	24.5
107	0	17.99374	17.75	13	22.75
108	0	10.423523	9.75	6	12.666667