## htsjdk.samtools.metrics.StringHeader # CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/data/Runs/415630/output/415630-20250125_0254/415630-L3455-Z0005-CATGTATCCTCTGAT/415630-L3455-Z0005-CATGTATCCTCTGAT_sample.sam.gz OUTPUT=/data/Runs/415630/output/415630-20250125_0254/415630-L3455-Z0005-CATGTATCCTCTGAT/415630-L3455-Z0005-CATGTATCCTCTGAT_FlowQ.metric INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false ## htsjdk.samtools.metrics.StringHeader # Started on: Sun Jan 26 12:26:49 EST 2025 ## METRICS CLASS picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow TOTAL_READS PF_READS MEAN_READ_LENGTH_IN_FLOWS PF_FLOWS PF_Q20_FLOWS PCT_PF_Q20_FLOWS PF_Q30_FLOWS PCT_PF_Q30_FLOWS Q20_EQUIVALENT_YIELD PF_Q20_EQUIVALENT_YIELD 1000358 1000358 295 295513010 258129002 0.873495 193231351 0.653884 434781029 434781029 ## HISTOGRAM java.lang.Integer KEY QUAL_COUNT MEAN_CYCLE_QUAL MEDIAN_CYCLE_QUAL Q25_CYCLE_QUAL Q75_CYCLE_QUAL 0 166955 30.538308 32.333333 28.333333 34.25 1 297866 30.988603 33 28.5 35 2 144948 30.793363 32.5 28 35 3 3244829 30.773781 32.5 28 35 4 75918 30.706977 32.5 28 35 5 137487 30.724937 32.5 28 35 6 4056128 30.638963 32.5 27.75 35 7 78428 30.649855 32.5 27.75 35 8 156061 30.769461 32.5 28 35 9 5023442 30.72605 32.5 28 35 10 63237 30.699085 32.5 27.75 35 11 134186 30.61517 32.5 27.75 35 12 5533784 30.648048 32.5 27.75 35 13 277302 30.722948 32.5 27.75 35 14 1219029 30.71902 32.5 27.75 35 15 4188668 30.658864 32.5 27.75 35 16 1236139 30.603092 32.5 27.5 35 17 4718193 30.605892 32.5 27.5 35 18 61266 30.564257 32.5 27.5 35 19 6570142 30.567673 32.5 27.5 35 20 1743390 30.483483 32.5 27.5 35 21 968830 30.435621 32.5 27.5 35 22 11044243 30.42405 32.5 27.5 35 23 39 30.315888 32.5 27 35 24 171859 30.289382 32.5 27 35 25 49314693 30.21446 32.25 26.75 35 26 0 30.071147 31.75 26.75 35 27 1561 29.978663 31.75 26.75 35 28 277889 29.847448 31.75 26.25 35 29 1375147 29.845259 31.75 26.25 35 30 11622081 29.811449 31.75 26.25 35 31 0 29.747467 31.75 26 35 32 17614917 29.705326 31.75 26 35 33 0 29.624784 31.75 26 35 34 0 29.566951 31.75 26 35 35 163994353 29.536462 31.75 25.75 35 36 0 29.481513 31.75 25.5 34.25 37 0 29.422085 31.75 25.25 34.25 38 0 29.344699 31.25 25.25 34.25 39 0 29.309145 31.25 25.25 34.25 40 0 29.30564 31.25 25.25 34.25 41 0 29.218171 31.25 25 34.25 42 0 29.197165 31.25 25 34.25 43 0 29.183652 31.25 25 34.25 44 0 29.18025 31.25 25 34.25 45 0 29.149746 31.25 25 34.25 46 0 29.079771 31 25 34.25 47 0 29.085972 31 25 34.25 48 0 29.060192 31 25 34.25 49 0 29.072433 31 25 34.25 50 0 29.057778 31 25 34.25 51 0 29.010972 31 25 34.25 52 0 28.97647 31 25 34.25 53 0 28.885339 31 25 34.25 54 0 28.907152 31 25 34.25 55 0 28.827604 31 25 34.25 56 0 28.806155 30.75 25 34.25 57 0 28.761409 30.5 25 34.25 58 0 28.73186 30.5 25 34.25 59 0 28.777337 30.5 25 34.25 60 0 28.761309 30.5 25 34.25 61 0 28.749801 30.5 25 34.25 62 0 28.737951 30.5 25 34.25 63 0 28.806022 31 25 34.25 64 0 28.826247 31 25 34.25 65 0 28.882615 31 25.25 34.25 66 0 28.888748 31 25.25 34.25 67 0 28.829165 31 25.25 34.25 68 0 28.826397 31 25.25 34.25 69 0 28.771418 31 25 34.25 70 0 28.660616 31 25 34.25 71 0 28.615897 31 25 34.25 72 0 28.480165 30.5 24.5 34.25 73 0 28.389794 30.5 24.5 34.25 74 0 28.237734 30.25 24.25 34.25 75 0 28.088424 30 24 34.25 76 0 27.939868 30 23.75 34.25 77 0 27.782096 30 23.5 34.25 78 0 27.591786 29.25 23 33.75 79 0 27.335735 29.25 22.75 33.75 80 0 27.150574 29.25 22.25 33.75 81 0 26.974032 28.75 22 33.75 82 0 26.768205 28.5 21.75 33.5 83 0 26.566413 28 21.25 33 84 0 26.342237 27.75 21 32.5 85 0 26.15619 27.5 21 32.5 86 0 25.973324 27.25 20.5 32.5 87 0 25.714389 26.75 20.25 32.5 88 0 25.519324 26.75 20 32.5 89 0 25.29478 26.25 19.75 31.75 90 0 24.98 26 19.5 31.75 91 0 24.645682 25.25 19 31.75 92 0 24.400064 25 18.75 31 93 0 23.915484 24.75 18 30.25 94 0 23.171781 24 17.25 29.75 95 0 22.218996 22.5 16.25 28.5 96 0 20.618874 20.5 14.75 26.75 97 0 22.565689 23.5 18 27.75 98 0 21.847381 22 17 27 99 0 21.833333 22 20.5 27.75 100 0 26.25 28.75 22.75 35 101 0 25.1875 29 23.75 35 102 0 35 35 35 35