SUMMARISING RUN PARAMETERS
==========================
Input filename: KO-N4371-2.end1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.4.4
Cutadapt version: 1.16
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1
Output file will be GZIP compressed


This is cutadapt 1.16 with Python 2.7.13
Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA KO-N4371-2.end1.fastq.gz
Running on 1 core
Trimming 1 adapter with at most 10.0% errors in single-end mode ...
Finished in 779.08 s (34 us/read; 1.78 M reads/minute).

=== Summary ===

Total reads processed:              23,086,373
Reads with adapters:                 9,112,040 (39.5%)
Reads written (passing filters):    23,086,373 (100.0%)

Total basepairs processed: 1,487,075,235 bp
Quality-trimmed:                  15,374 bp (0.0%)
Total written (filtered):  1,470,875,047 bp (98.9%)

=== Adapter 1 ===

Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 9112040 times.

No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1

Bases preceding removed adapters:
  A: 26.7%
  C: 27.8%
  G: 23.4%
  T: 22.1%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	7089346	5771593.2	0	7089346
2	1361638	1442898.3	0	1361638
3	379119	360724.6	0	379119
4	82793	90181.1	0	82793
5	22232	22545.3	0	22232
6	12405	5636.3	0	12405
7	4395	1409.1	0	4395
8	3410	352.3	0	3410
9	3501	88.1	0	2776 725
10	3461	22.0	1	2490 971
11	3854	5.5	1	2660 1194
12	3431	1.4	1	2778 653
13	3289	1.4	1	2733 556
14	3661	1.4	1	2716 945
15	3260	1.4	1	2334 926
16	2636	1.4	1	2181 455
17	2712	1.4	1	2287 425
18	2755	1.4	1	2559 196
19	3336	1.4	1	2976 360
20	3527	1.4	1	3154 373
21	3934	1.4	1	3709 225
22	4935	1.4	1	4131 804
23	5286	1.4	1	5032 254
24	5054	1.4	1	4784 270
25	4245	1.4	1	4059 186
26	3326	1.4	1	3107 219
27	3425	1.4	1	3084 341
28	3594	1.4	1	3305 289
29	4277	1.4	1	3991 286
30	4738	1.4	1	4512 226
31	4866	1.4	1	4635 231
32	5361	1.4	1	5178 183
33	7327	1.4	1	6999 328
34	7581	1.4	1	7346 235
35	7023	1.4	1	6798 225
36	5379	1.4	1	5112 267
37	4352	1.4	1	4174 178
38	4778	1.4	1	4547 231
39	4557	1.4	1	4363 194
40	3332	1.4	1	3071 261
41	1919	1.4	1	1740 179
42	1239	1.4	1	1085 154
43	1347	1.4	1	871 476
44	457	1.4	1	288 169
45	620	1.4	1	313 307
46	600	1.4	1	384 216
47	463	1.4	1	298 165
48	938	1.4	1	606 332
49	1321	1.4	1	1135 186
50	1564	1.4	1	128 1436
51	332	1.4	1	21 311
52	236	1.4	1	14 222
53	439	1.4	1	4 435
54	129	1.4	1	7 122
55	409	1.4	1	5 404
56	236	1.4	1	1 235
57	134	1.4	1	2 132
58	385	1.4	1	3 382
59	206	1.4	1	1 205
60	984	1.4	1	1 983
61	180	1.4	1	6 174
62	259	1.4	1	55 204
63	433	1.4	1	5 428
64	123	1.4	1	18 105
65	133	1.4	1	9 124
66	193	1.4	1	12 181
67	184	1.4	1	11 173
68	377	1.4	1	5 372
69	84	1.4	1	5 79
70	80	1.4	1	5 75
71	57	1.4	1	8 49
72	99	1.4	1	24 75
73	381	1.4	1	24 357
74	164	1.4	1	80 84
75	3114	1.4	1	2909 205
76	90	1.4	1	7 83


RUN STATISTICS FOR INPUT FILE: KO-N4371-2.end1.fastq.gz
=============================================
23086373 sequences processed in total