SUMMARISING RUN PARAMETERS
==========================
Input filename: KO-N4371-1.end1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.4.4
Cutadapt version: 1.16
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1
Output file will be GZIP compressed


This is cutadapt 1.16 with Python 2.7.13
Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA KO-N4371-1.end1.fastq.gz
Running on 1 core
Trimming 1 adapter with at most 10.0% errors in single-end mode ...
Finished in 1053.05 s (35 us/read; 1.73 M reads/minute).

=== Summary ===

Total reads processed:              30,394,942
Reads with adapters:                12,591,962 (41.4%)
Reads written (passing filters):    30,394,942 (100.0%)

Total basepairs processed: 2,001,326,638 bp
Quality-trimmed:                 866,111 bp (0.0%)
Total written (filtered):  1,947,232,356 bp (97.3%)

=== Adapter 1 ===

Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 12591962 times.

No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1

Bases preceding removed adapters:
  A: 25.1%
  C: 28.8%
  G: 23.4%
  T: 22.4%
  none/other: 0.4%

Overview of removed sequences
length	count	expect	max.err	error counts
1	8745855	7598735.5	0	8745855
2	1765344	1899683.9	0	1765344
3	503088	474921.0	0	503088
4	126765	118730.2	0	126765
5	47862	29682.6	0	47862
6	28384	7420.6	0	28384
7	21607	1855.2	0	21607
8	19353	463.8	0	19353
9	23286	115.9	0	22335 951
10	27413	29.0	1	23469 3944
11	29783	7.2	1	24411 5372
12	31255	1.8	1	26619 4636
13	31137	1.8	1	25784 5353
14	30300	1.8	1	25384 4916
15	30004	1.8	1	24484 5520
16	24360	1.8	1	20508 3852
17	24849	1.8	1	20702 4147
18	24485	1.8	1	20993 3492
19	27857	1.8	1	25434 2423
20	32327	1.8	1	29015 3312
21	39257	1.8	1	35764 3493
22	42392	1.8	1	37441 4951
23	43810	1.8	1	38863 4947
24	48293	1.8	1	43088 5205
25	48269	1.8	1	42788 5481
26	39886	1.8	1	35330 4556
27	33128	1.8	1	29128 4000
28	30307	1.8	1	26656 3651
29	36883	1.8	1	32380 4503
30	44253	1.8	1	39356 4897
31	44580	1.8	1	39479 5101
32	44604	1.8	1	40010 4594
33	50635	1.8	1	44619 6016
34	61934	1.8	1	54901 7033
35	70486	1.8	1	64011 6475
36	53120	1.8	1	48099 5021
37	45102	1.8	1	40344 4758
38	32923	1.8	1	29028 3895
39	38616	1.8	1	34137 4479
40	34904	1.8	1	30964 3940
41	21337	1.8	1	18802 2535
42	12849	1.8	1	11243 1606
43	8330	1.8	1	6926 1404
44	2695	1.8	1	2156 539
45	3171	1.8	1	2428 743
46	2257	1.8	1	1804 453
47	2904	1.8	1	2340 564
48	3124	1.8	1	2423 701
49	4632	1.8	1	3961 671
50	6693	1.8	1	4349 2344
51	1001	1.8	1	525 476
52	424	1.8	1	122 302
53	737	1.8	1	136 601
54	234	1.8	1	44 190
55	565	1.8	1	38 527
56	314	1.8	1	30 284
57	264	1.8	1	46 218
58	547	1.8	1	39 508
59	328	1.8	1	35 293
60	1329	1.8	1	53 1276
61	299	1.8	1	84 215
62	386	1.8	1	137 249
63	1052	1.8	1	482 570
64	317	1.8	1	166 151
65	461	1.8	1	274 187
66	579	1.8	1	300 279
67	575	1.8	1	272 303
68	885	1.8	1	292 593
69	676	1.8	1	533 143
70	462	1.8	1	330 132
71	490	1.8	1	376 114
72	763	1.8	1	596 167
73	1555	1.8	1	970 585
74	1625	1.8	1	1357 268
75	7712	1.8	1	7028 684
76	21694	1.8	1	19537 2157


RUN STATISTICS FOR INPUT FILE: KO-N4371-1.end1.fastq.gz
=============================================
30394942 sequences processed in total