SUMMARISING RUN PARAMETERS
==========================
Input filename: KO-N4293-2.end1.fastq.gz
Trimming mode: paired-end
Trim Galore version: 0.4.4
Cutadapt version: 1.16
Quality Phred score cutoff: 20
Quality encoding type selected: ASCII+33
Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected)
Maximum trimming error rate: 0.1 (default)
Minimum required adapter overlap (stringency): 1 bp
Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1
Output file will be GZIP compressed


This is cutadapt 1.16 with Python 2.7.13
Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA KO-N4293-2.end1.fastq.gz
Running on 1 core
Trimming 1 adapter with at most 10.0% errors in single-end mode ...
Finished in 676.31 s (34 us/read; 1.76 M reads/minute).

=== Summary ===

Total reads processed:              19,786,147
Reads with adapters:                 7,790,734 (39.4%)
Reads written (passing filters):    19,786,147 (100.0%)

Total basepairs processed: 1,281,983,454 bp
Quality-trimmed:                  10,657 bp (0.0%)
Total written (filtered):  1,268,657,282 bp (99.0%)

=== Adapter 1 ===

Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 7790734 times.

No. of allowed errors:
0-9 bp: 0; 10-12 bp: 1

Bases preceding removed adapters:
  A: 26.5%
  C: 27.8%
  G: 23.5%
  T: 22.2%
  none/other: 0.0%

Overview of removed sequences
length	count	expect	max.err	error counts
1	6064234	4946536.8	0	6064234
2	1176330	1236634.2	0	1176330
3	328109	309158.5	0	328109
4	71676	77289.6	0	71676
5	18845	19322.4	0	18845
6	10767	4830.6	0	10767
7	3753	1207.7	0	3753
8	2696	301.9	0	2696
9	2704	75.5	0	2084 620
10	2670	18.9	1	1890 780
11	2872	4.7	1	1893 979
12	2569	1.2	1	2091 478
13	2523	1.2	1	2090 433
14	2716	1.2	1	1882 834
15	2527	1.2	1	1748 779
16	1984	1.2	1	1568 416
17	1934	1.2	1	1563 371
18	2011	1.2	1	1836 175
19	2385	1.2	1	2088 297
20	2579	1.2	1	2302 277
21	2772	1.2	1	2606 166
22	3777	1.2	1	3113 664
23	3794	1.2	1	3592 202
24	3754	1.2	1	3541 213
25	2982	1.2	1	2842 140
26	2438	1.2	1	2218 220
27	2367	1.2	1	2060 307
28	2686	1.2	1	2470 216
29	3203	1.2	1	2943 260
30	3529	1.2	1	3387 142
31	3568	1.2	1	3372 196
32	3813	1.2	1	3625 188
33	5208	1.2	1	4950 258
34	5560	1.2	1	5363 197
35	4690	1.2	1	4494 196
36	3634	1.2	1	3449 185
37	2917	1.2	1	2770 147
38	3385	1.2	1	3142 243
39	3036	1.2	1	2863 173
40	2147	1.2	1	1963 184
41	1177	1.2	1	1026 151
42	736	1.2	1	598 138
43	963	1.2	1	542 421
44	365	1.2	1	201 164
45	478	1.2	1	203 275
46	402	1.2	1	221 181
47	444	1.2	1	250 194
48	656	1.2	1	393 263
49	877	1.2	1	729 148
50	1292	1.2	1	80 1212
51	263	1.2	1	21 242
52	194	1.2	1	25 169
53	385	1.2	1	4 381
54	116	1.2	1	6 110
55	378	1.2	1	5 373
56	158	1.2	1	3 155
57	148	1.2	1	5 143
58	353	1.2	1	2 351
59	207	1.2	1	1 206
60	820	1.2	1	4 816
61	137	1.2	1	4 133
62	180	1.2	1	42 138
63	361	1.2	1	6 355
64	109	1.2	1	17 92
65	102	1.2	1	7 95
66	161	1.2	1	7 154
67	162	1.2	1	6 156
68	334	1.2	1	1 333
69	68	1.2	1	4 64
70	79	1.2	1	6 73
71	32	1.2	1	4 28
72	91	1.2	1	18 73
73	331	1.2	1	21 310
74	158	1.2	1	78 80
75	2803	1.2	1	2624 179
76	70	1.2	1	5 65


RUN STATISTICS FOR INPUT FILE: KO-N4293-2.end1.fastq.gz
=============================================
19786147 sequences processed in total