SUMMARISING RUN PARAMETERS ========================== Input filename: A-N4293-2.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-N4293-2.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 974.34 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 27,006,792 Reads with adapters: 10,577,843 (39.2%) Reads written (passing filters): 27,006,792 (100.0%) Total basepairs processed: 1,741,468,989 bp Quality-trimmed: 15,694 bp (0.0%) Total written (filtered): 1,723,264,049 bp (99.0%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 10577843 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 26.9% C: 27.5% G: 23.2% T: 22.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 8221534 6751698.0 0 8221534 2 1593027 1687924.5 0 1593027 3 451502 421981.1 0 451502 4 99725 105495.3 0 99725 5 25984 26373.8 0 25984 6 14552 6593.5 0 14552 7 5148 1648.4 0 5148 8 3752 412.1 0 3752 9 3713 103.0 0 2918 795 10 3735 25.8 1 2532 1203 11 4145 6.4 1 2733 1412 12 3467 1.6 1 2800 667 13 3470 1.6 1 2881 589 14 3830 1.6 1 2558 1272 15 3436 1.6 1 2322 1114 16 2920 1.6 1 2295 625 17 2816 1.6 1 2285 531 18 3011 1.6 1 2768 243 19 3480 1.6 1 3061 419 20 3864 1.6 1 3392 472 21 4065 1.6 1 3836 229 22 5126 1.6 1 4119 1007 23 5328 1.6 1 5016 312 24 5189 1.6 1 4885 304 25 4395 1.6 1 4163 232 26 3707 1.6 1 3461 246 27 3604 1.6 1 3221 383 28 3886 1.6 1 3546 340 29 4652 1.6 1 4280 372 30 5125 1.6 1 4867 258 31 5283 1.6 1 5040 243 32 5623 1.6 1 5393 230 33 7214 1.6 1 6859 355 34 7943 1.6 1 7698 245 35 7023 1.6 1 6787 236 36 5603 1.6 1 5336 267 37 4816 1.6 1 4590 226 38 5057 1.6 1 4763 294 39 4725 1.6 1 4471 254 40 3223 1.6 1 2980 243 41 1823 1.6 1 1647 176 42 1074 1.6 1 921 153 43 1350 1.6 1 796 554 44 559 1.6 1 348 211 45 759 1.6 1 368 391 46 687 1.6 1 439 248 47 657 1.6 1 420 237 48 1043 1.6 1 659 384 49 1373 1.6 1 1158 215 50 1626 1.6 1 127 1499 51 376 1.6 1 37 339 52 283 1.6 1 14 269 53 508 1.6 1 9 499 54 168 1.6 1 10 158 55 434 1.6 1 4 430 56 254 1.6 1 8 246 57 194 1.6 1 5 189 58 396 1.6 1 2 394 59 259 1.6 1 1 258 60 1024 1.6 1 4 1020 61 193 1.6 1 3 190 62 247 1.6 1 23 224 63 404 1.6 1 3 401 64 154 1.6 1 7 147 65 159 1.6 1 9 150 66 229 1.6 1 9 220 67 217 1.6 1 3 214 68 460 1.6 1 10 450 69 72 1.6 1 1 71 70 104 1.6 1 5 99 71 60 1.6 1 4 56 72 107 1.6 1 7 100 73 402 1.6 1 6 396 74 146 1.6 1 42 104 75 1277 1.6 1 1110 167 76 67 1.6 1 2 65 RUN STATISTICS FOR INPUT FILE: A-N4293-2.end1.fastq.gz ============================================= 27006792 sequences processed in total