SUMMARISING RUN PARAMETERS ========================== Input filename: A-15171-1.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-15171-1.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 898.95 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 25,789,699 Reads with adapters: 10,235,752 (39.7%) Reads written (passing filters): 25,789,699 (100.0%) Total basepairs processed: 1,628,355,066 bp Quality-trimmed: 12,171 bp (0.0%) Total written (filtered): 1,610,634,389 bp (98.9%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 10235752 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 28.2% C: 27.7% G: 22.7% T: 21.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 8106357 6447424.8 0 8106357 2 1465944 1611856.2 0 1465944 3 366408 402964.0 0 366408 4 79958 100741.0 0 79958 5 20476 25185.3 0 20476 6 10955 6296.3 0 10955 7 4660 1574.1 0 4660 8 4095 393.5 0 4095 9 4032 98.4 0 3281 751 10 3986 24.6 1 3026 960 11 4876 6.1 1 3109 1767 12 4088 1.5 1 3198 890 13 3964 1.5 1 3303 661 14 5106 1.5 1 3230 1876 15 4498 1.5 1 2771 1727 16 3667 1.5 1 2818 849 17 3545 1.5 1 2877 668 18 3342 1.5 1 3115 227 19 4107 1.5 1 3585 522 20 4390 1.5 1 3841 549 21 4695 1.5 1 4418 277 22 5958 1.5 1 4597 1361 23 5835 1.5 1 5503 332 24 6308 1.5 1 5934 374 25 5281 1.5 1 5100 181 26 4391 1.5 1 4145 246 27 4192 1.5 1 3813 379 28 4463 1.5 1 4139 324 29 5280 1.5 1 4962 318 30 5802 1.5 1 5606 196 31 5650 1.5 1 5408 242 32 5745 1.5 1 5539 206 33 7576 1.5 1 7246 330 34 7984 1.5 1 7759 225 35 7520 1.5 1 7356 164 36 5394 1.5 1 5175 219 37 4648 1.5 1 4502 146 38 4973 1.5 1 4662 311 39 4165 1.5 1 4001 164 40 2788 1.5 1 2600 188 41 1450 1.5 1 1292 158 42 834 1.5 1 695 139 43 1055 1.5 1 533 522 44 433 1.5 1 270 163 45 714 1.5 1 286 428 46 587 1.5 1 282 305 47 485 1.5 1 286 199 48 851 1.5 1 488 363 49 1163 1.5 1 916 247 50 2356 1.5 1 92 2264 51 384 1.5 1 19 365 52 348 1.5 1 10 338 53 585 1.5 1 4 581 54 111 1.5 1 1 110 55 525 1.5 1 2 523 56 219 1.5 1 1 218 57 175 1.5 1 1 174 58 503 1.5 1 2 501 59 355 1.5 1 1 354 60 1338 1.5 1 1 1337 61 173 1.5 1 2 171 62 280 1.5 1 20 260 63 435 1.5 1 2 433 64 94 1.5 1 1 93 65 129 1.5 1 5 124 66 144 1.5 1 4 140 67 276 1.5 1 5 271 68 418 1.5 1 5 413 69 69 1.5 1 1 68 70 58 1.5 1 3 55 71 35 1.5 1 1 34 72 103 1.5 1 7 96 73 436 1.5 1 17 419 74 128 1.5 1 32 96 75 1353 1.5 1 1171 182 76 48 1.5 1 3 45 RUN STATISTICS FOR INPUT FILE: A-15171-1.end1.fastq.gz ============================================= 25789699 sequences processed in total