SUMMARISING RUN PARAMETERS ========================== Input filename: A-13190-1.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-13190-1.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1200.58 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 33,292,159 Reads with adapters: 13,099,080 (39.3%) Reads written (passing filters): 33,292,159 (100.0%) Total basepairs processed: 2,131,725,667 bp Quality-trimmed: 12,555 bp (0.0%) Total written (filtered): 2,110,437,014 bp (99.0%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 13099080 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 27.5% C: 27.5% G: 23.0% T: 22.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 10294530 8323039.8 0 10294530 2 1946938 2080759.9 0 1946938 3 524076 520190.0 0 524076 4 114914 130047.5 0 114914 5 29417 32511.9 0 29417 6 16376 8128.0 0 16376 7 5514 2032.0 0 5514 8 4142 508.0 0 4142 9 4108 127.0 0 3043 1065 10 3871 31.7 1 2557 1314 11 4843 7.9 1 2850 1993 12 4077 2.0 1 3003 1074 13 3774 2.0 1 2995 779 14 4478 2.0 1 2652 1826 15 4038 2.0 1 2369 1669 16 3007 2.0 1 2223 784 17 3034 2.0 1 2296 738 18 2841 2.0 1 2600 241 19 3545 2.0 1 2964 581 20 3806 2.0 1 3239 567 21 3940 2.0 1 3655 285 22 5681 2.0 1 4265 1416 23 5735 2.0 1 5336 399 24 5700 2.0 1 5271 429 25 4454 2.0 1 4218 236 26 3663 2.0 1 3358 305 27 3565 2.0 1 3075 490 28 3738 2.0 1 3317 421 29 4419 2.0 1 4025 394 30 4683 2.0 1 4434 249 31 4728 2.0 1 4454 274 32 5033 2.0 1 4772 261 33 7232 2.0 1 6789 443 34 7334 2.0 1 7050 284 35 6364 2.0 1 6109 255 36 4894 2.0 1 4576 318 37 3669 2.0 1 3447 222 38 4248 2.0 1 3862 386 39 3945 2.0 1 3665 280 40 2977 2.0 1 2681 296 41 1645 2.0 1 1422 223 42 1060 2.0 1 861 199 43 1412 2.0 1 752 660 44 553 2.0 1 288 265 45 760 2.0 1 280 480 46 583 2.0 1 247 336 47 519 2.0 1 264 255 48 1003 2.0 1 479 524 49 1087 2.0 1 821 266 50 2402 2.0 1 96 2306 51 498 2.0 1 29 469 52 345 2.0 1 12 333 53 737 2.0 1 4 733 54 162 2.0 1 7 155 55 574 2.0 1 2 572 56 326 2.0 1 6 320 57 243 2.0 1 3 240 58 623 2.0 1 4 619 59 381 2.0 1 2 379 60 1588 2.0 1 4 1584 61 248 2.0 1 3 245 62 288 2.0 1 23 265 63 606 2.0 1 5 601 64 168 2.0 1 11 157 65 151 2.0 1 5 146 66 255 2.0 1 4 251 67 304 2.0 1 5 299 68 539 2.0 1 1 538 69 107 2.0 1 2 105 70 102 2.0 1 5 97 71 75 2.0 1 5 70 72 109 2.0 1 12 97 73 537 2.0 1 18 519 74 148 2.0 1 25 123 75 1532 2.0 1 1287 245 76 79 2.0 1 0 79 RUN STATISTICS FOR INPUT FILE: A-13190-1.end1.fastq.gz ============================================= 33292159 sequences processed in total