SUMMARISING RUN PARAMETERS ========================== Input filename: A-13189-1.end1.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.16 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.16 with Python 2.7.13 Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA A-13189-1.end1.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1161.04 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 32,315,377 Reads with adapters: 12,751,672 (39.5%) Reads written (passing filters): 32,315,377 (100.0%) Total basepairs processed: 2,056,122,463 bp Quality-trimmed: 14,452 bp (0.0%) Total written (filtered): 2,034,139,382 bp (98.9%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 12751672 times. No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 28.1% C: 27.9% G: 22.7% T: 21.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 10096945 8078844.2 0 10096945 2 1843186 2019711.1 0 1843186 3 449665 504927.8 0 449665 4 97891 126231.9 0 97891 5 24552 31558.0 0 24552 6 13500 7889.5 0 13500 7 5539 1972.4 0 5539 8 5188 493.1 0 5188 9 4937 123.3 0 4018 919 10 4645 30.8 1 3526 1119 11 5844 7.7 1 3855 1989 12 5429 1.9 1 4228 1201 13 5208 1.9 1 4312 896 14 6302 1.9 1 3972 2330 15 5552 1.9 1 3616 1936 16 4127 1.9 1 3180 947 17 4225 1.9 1 3394 831 18 3802 1.9 1 3566 236 19 4738 1.9 1 4130 608 20 5109 1.9 1 4524 585 21 5484 1.9 1 5185 299 22 7607 1.9 1 6027 1580 23 8285 1.9 1 7927 358 24 8100 1.9 1 7712 388 25 6610 1.9 1 6382 228 26 5090 1.9 1 4766 324 27 5012 1.9 1 4496 516 28 4992 1.9 1 4601 391 29 5997 1.9 1 5608 389 30 6166 1.9 1 5921 245 31 6087 1.9 1 5860 227 32 6495 1.9 1 6258 237 33 9367 1.9 1 8902 465 34 9950 1.9 1 9686 264 35 9028 1.9 1 8817 211 36 6692 1.9 1 6424 268 37 5069 1.9 1 4867 202 38 5504 1.9 1 5172 332 39 5149 1.9 1 4924 225 40 3386 1.9 1 3129 257 41 1940 1.9 1 1754 186 42 1054 1.9 1 904 150 43 1542 1.9 1 752 790 44 556 1.9 1 345 211 45 856 1.9 1 327 529 46 599 1.9 1 286 313 47 487 1.9 1 271 216 48 1011 1.9 1 476 535 49 1268 1.9 1 1025 243 50 3407 1.9 1 98 3309 51 506 1.9 1 17 489 52 385 1.9 1 5 380 53 859 1.9 1 5 854 54 141 1.9 1 4 137 55 748 1.9 1 6 742 56 305 1.9 1 4 301 57 221 1.9 1 1 220 58 732 1.9 1 0 732 59 477 1.9 1 0 477 60 2147 1.9 1 3 2144 61 252 1.9 1 3 249 62 379 1.9 1 30 349 63 796 1.9 1 1 795 64 99 1.9 1 9 90 65 182 1.9 1 4 178 66 187 1.9 1 4 183 67 338 1.9 1 4 334 68 537 1.9 1 7 530 69 99 1.9 1 2 97 70 89 1.9 1 2 87 71 53 1.9 1 3 50 72 114 1.9 1 4 110 73 714 1.9 1 12 702 74 174 1.9 1 46 128 75 1894 1.9 1 1627 267 76 70 1.9 1 1 69 RUN STATISTICS FOR INPUT FILE: A-13189-1.end1.fastq.gz ============================================= 32315377 sequences processed in total