#sgRNA_ID	sgRNA	chr	left	right	strand	motif	motifStrand	ChIP-seq_peak_pos	cutting_efficiency_score	cutting_specificity_score	offtargets_sum	offtargets_summary
sgRNA_1	GGTCGTCTTTACTGACTTAA	chr10	117152291	117152313	+	Nanog	+	117152434	28	0.62234043	4	2:0|3:4
sgRNA_2	TTAAGTCAGTAAAGACGACC	chr10	117152288	117152310	-	Nanog	+	117152434	65	0.53047692	5	2:1|3:4
sgRNA_3	ACAGCGGGTGGGCCATTAGG	chr10	117452499	117452521	-	Nanog	+	117452494	53	0.46944101	4	2:0|3:4
sgRNA_4	AGCACAGCGGGTGGGCCATT	chr10	117452502	117452524	-	Nanog	+	117452494	42	0.84935632	3	2:0|3:3
sgRNA_5	ATGGGTGGTTGACTTAGCCC	chr10	121016349	121016371	+	Nanog	+	121016272	56	0.6241346	3	2:0|3:3
sgRNA_6	AGAGCGATTAGAGAGTAGTC	chr10	121569264	121569286	+	Nanog	-	121569290	54	0.72654691	3	2:0|3:3
sgRNA_7	TCGCTCTGCGCATACGTTAG	chr10	121569248	121569270	-	Nanog	-	121569290	44	0.96096096	1	2:0|3:1
sgRNA_8	GCGATTAGAGAGTAGTCAGG	chr10	121569267	121569289	+	Nanog	-	121569290	66	0.67509025	1	2:0|3:1
sgRNA_9	CGCTCTGCGCATACGTTAGT	chr10	121569247	121569269	-	Nanog	-	121569290	41	1.0	1	2:0|3:1
sgRNA_10	TGAAAGCTTGGGGCGCTAAT	chr10	127121181	127121203	-	Nanog	-	127121160	40	0.75806005	3	2:0|3:3
sgRNA_11	ATGAAAGCTTGGGGCGCTAA	chr10	127121182	127121204	-	Nanog	-	127121160	28	0.55860047	4	2:0|3:4
sgRNA_12	AAGCTTGGGGCGCTAATGGG	chr10	127121178	127121200	-	Nanog	-	127121160	51	0.88047809	2	2:0|3:2
sgRNA_13	AGCTTGGGGCGCTAATGGGC	chr10	127121177	127121199	-	Nanog	-	127121160	50	0.81632653	1	2:0|3:1
sgRNA_14	TTAGCGCCCCAAGCTTTCAT	chr10	127121185	127121207	+	Nanog	-	127121160	45	0.68353598	2	2:0|3:2
sgRNA_15	GGGGCGCTAATGGGCGGGTG	chr10	127121172	127121194	-	Nanog	-	127121160	46	0.70761219	3	2:2|3:1
sgRNA_16	GTAAATCACCCATTAATTCT	chr10	127907510	127907532	-	Nanog	+	127907652	50	0.75740432	4	2:0|3:4
sgRNA_17	TTTGTCATGCGGGCAGAAAC	chr10	24086120	24086142	+	Nanog	-	24086129	39	0.51694754	5	2:0|3:5
sgRNA_18	CATTAACATAGGGACCTAGG	chr10	25279600	25279622	-	Nanog	+	25279631	74	0.81661891	1	2:0|3:1
sgRNA_19	CCATTAACATAGGGACCTAG	chr10	25279601	25279623	-	Nanog	+	25279631	70	0.55869378	4	2:0|3:4
sgRNA_20	GTTTTACCCCTTTCAGTCCA	chr10	3741810	3741832	+	Nanog	+	3741896	48	0.35996363	5	2:0|3:5
sgRNA_21	AATTTCAATACAGCCCTAAT	chr10	44435028	44435050	-	Nanog	-	44435073	41	0.45749787	4	2:0|3:4
sgRNA_22	TGAACAATCCTGTTAATCGC	chr10	62893044	62893066	-	Nanog	-	62893062	48	1.0	3	2:0|3:3
sgRNA_23	AACAATCCTGTTAATCGCTG	chr10	62893042	62893064	-	Nanog	-	62893062	63	0.49486338	5	2:0|3:5
sgRNA_24	TAAACTCCCCAGCGATTAAC	chr10	62893036	62893058	+	Nanog	-	62893062	36	0.49094997	3	2:0|3:3
sgRNA_25	GAACAATCCTGTTAATCGCT	chr10	62893043	62893065	-	Nanog	-	62893062	49	0.47272944	5	2:0|3:5
sgRNA_26	ACCGCTGGGCTGCTAATGGC	chr10	70433262	70433284	-	Nanog	-	70433440	35	0.86802828	2	2:0|3:2
sgRNA_27	TGCTACCGCTGGGCTGCTAA	chr10	70433266	70433288	-	Nanog	-	70433440	39	0.54464121	4	2:0|3:4
sgRNA_28	TGGGTGACCCCCAAAGACGT	chr10	85538538	85538560	-	Nanog	-	85538579	61	0.83505296	3	2:0|3:3
sgRNA_29	GTCTTTGGGGGTCACCCATT	chr10	85538543	85538565	+	Nanog	-	85538579	42	0.8450189	4	2:0|3:4
sgRNA_30	GGGTGACCCCCAAAGACGTT	chr10	85538537	85538559	-	Nanog	-	85538579	51	0.64246632	3	2:1|3:2
sgRNA_31	TAAGCTCTTCCTACGACAGT	chr10	95236345	95236367	-	Nanog	+	95236280	59	0.77816749	5	2:0|3:5
sgRNA_32	TGCGAGGACTAATGGCCTCA	chr11	114129513	114129535	+	Nanog	+	114129448	54	0.89298839	1	2:0|3:1
sgRNA_33	GGACTAATGGCCTCAGGGGC	chr11	114129518	114129540	+	Nanog	+	114129448	33	0.48340229	5	2:0|3:5
sgRNA_34	GCGAGGACTAATGGCCTCAG	chr11	114129514	114129536	+	Nanog	+	114129448	65	0.4192123	3	2:1|3:2
sgRNA_35	GCGCAAGCCGATTAAGAACC	chr11	117916105	117916127	-	Nanog	+	117916030	48	0.85981308	1	2:0|3:1
sgRNA_36	GCAGTAACCTGGTTCTTAAT	chr11	117916098	117916120	+	Nanog	+	117916030	36	0.88015763	4	2:0|3:4
sgRNA_37	TTAATCGGCTTGCGCAAAAT	chr11	117916113	117916135	+	Nanog	+	117916030	31	0.73785518	1	2:0|3:1
sgRNA_38	CTTTTACTATAGGCCAATTA	chr11	24630625	24630647	-	Nanog	+	24630709	26	0.77348472	5	2:0|3:5
sgRNA_39	AGGCCAATTAAGGATGGGTC	chr11	24630615	24630637	-	Nanog	+	24630709	46	0.78800204	4	2:0|3:4
sgRNA_40	ACTATAGGCCAATTAAGGAT	chr11	24630620	24630642	-	Nanog	+	24630709	55	0.54784339	4	2:0|3:4
sgRNA_41	GCGCTGGAGCAATTAAGGAG	chr11	28582236	28582258	+	Nanog	-	28582414	67	0.43932267	4	2:0|3:4
sgRNA_42	ATCGGCAACACCTTCTGCAG	chr11	44767532	44767554	+	Nanog	+	44767472	56	0.33167809	5	2:1|3:4
sgRNA_43	GTTCCCGTTCATTCCTTAAT	chr11	46217971	46217993	+	Nanog	+	46217966	33	0.6433008	2	2:0|3:2
sgRNA_44	AGTTCCCGTTCATTCCTTAA	chr11	46217970	46217992	+	Nanog	+	46217966	39	0.52690352	5	2:0|3:5
sgRNA_45	GTTAACCTTCATTCCCCTAA	chr11	54200456	54200478	-	Nanog	-	54200532	35	0.43867495	4	2:0|3:4
sgRNA_46	CTAATCGGTCAGAGGTGTTC	chr11	5506444	5506466	-	Nanog	-	5506401	37	0.71441531	3	2:0|3:3
sgRNA_47	GAGGAATGCTAATCGGTCAG	chr11	5506452	5506474	-	Nanog	-	5506401	66	0.58313367	4	2:0|3:4
sgRNA_48	TAATGCCGCTCTGCCCATTA	chr11	5942995	5943017	-	Nanog	+	5943064	30	0.62594635	4	2:0|3:4
sgRNA_49	TTAATGCCGCTCTGCCCATT	chr11	5942996	5943018	-	Nanog	+	5943064	26	0.86440678	1	2:0|3:1
sgRNA_50	AATGCCGCTCTGCCCATTAG	chr11	5942994	5943016	-	Nanog	+	5943064	60	0.33677657	5	2:0|3:5
sgRNA_51	AACAGCACAACACCCCTAAT	chr11	5942982	5943004	+	Nanog	+	5943064	46	0.55818074	3	2:0|3:3
sgRNA_52	GTCAGTCATTAATGGCCGAT	chr11	7532014	7532036	+	Nanog	+	7532039	56	0.73506042	3	2:0|3:3
sgRNA_53	TGTCAGTCATTAATGGCCGA	chr11	7532013	7532035	+	Nanog	+	7532039	55	0.41089357	5	2:0|3:5
sgRNA_54	TTTAATGGCCGTGAACCAAG	chr11	77891068	77891090	-	Nanog	-	77891092	67	0.86486486	1	2:0|3:1
sgRNA_55	CTTATTCTTAGCTAATCGCT	chr11	89955841	89955863	+	Nanog	+	89955897	50	0.55276757	4	2:0|3:4
sgRNA_56	GCCCATTAGCTGCTTAATTA	chr11	94958656	94958678	-	Nanog	+	94958578	22	0.46189323	5	2:0|3:5
sgRNA_57	TAGCTGCTTAATTATGGAAC	chr11	94958650	94958672	-	Nanog	+	94958578	35	0.56628406	4	2:0|3:4
sgRNA_58	GCCCATTAGCTGCTTAATTA	chr11	94958656	94958678	-	Nanog	+	94958778	22	0.46189323	5	2:0|3:5
sgRNA_59	TAGCTGCTTAATTATGGAAC	chr11	94958650	94958672	-	Nanog	+	94958778	35	0.56628406	4	2:0|3:4
sgRNA_60	TCGGCCATTAATTAGGAAAC	chr12	100242638	100242660	+	Nanog	-	100242547	43	0.95394737	1	2:0|3:1
sgRNA_61	GTCTGGCTCGGCCATTAATT	chr12	100242631	100242653	+	Nanog	-	100242547	32	0.50337079	2	2:0|3:2
sgRNA_62	TCATCCTAAGAGGGCCATTA	chr12	105087588	105087610	-	Nanog	+	105087565	28	0.559086	5	2:0|3:5
sgRNA_63	GAGCTTCCGGCTTTTTCTAA	chr12	12177684	12177706	-	Nanog	-	12177597	27	0.74732257	4	2:0|3:4
sgRNA_64	GCAATTAACCTTGTCCCTGC	chr12	13066966	13066988	+	Nanog	-	13066937	49	0.63371571	2	2:0|3:2
sgRNA_65	AGGCCATTAGAGAGTGACAA	chr12	13627383	13627405	+	Nanog	-	13627433	61	0.37445341	5	2:0|3:5
sgRNA_66	CTTGCTAAGAGTAGTGCTAA	chr12	3738932	3738954	+	Nanog	+	3738949	49	0.85561497	1	2:0|3:1
sgRNA_67	CTTGCTAAGAGTAGTGCTAA	chr12	3738932	3738954	+	Nanog	+	3739149	49	0.85561497	1	2:0|3:1
sgRNA_68	GTGTGAACTAGTGGCTTAAT	chr12	40836981	40837003	-	Nanog	-	40837103	34	0.74550479	3	2:0|3:3
sgRNA_69	AAGAAAGCGTCAACCCATTA	chr12	50120692	50120714	+	Nanog	-	50120820	31	0.65070423	3	2:0|3:3
sgRNA_70	GCCTTAATCGCTTCTCACTT	chr12	55836144	55836166	-	Nanog	-	55836202	47	0.58514923	3	2:0|3:3
sgRNA_71	TCCGAAGTGAGAAGCGATTA	chr12	55836143	55836165	+	Nanog	-	55836202	28	0.65106434	3	2:0|3:3
sgRNA_72	CACTGTTTGACCCATTAAAC	chr12	56473100	56473122	+	Nanog	-	56473070	35	0.52764187	5	2:1|3:4
sgRNA_73	GACCCATTAAACTGGTATTG	chr12	56473108	56473130	+	Nanog	-	56473070	48	0.44453044	5	2:0|3:5
sgRNA_74	GTACCTACCAGGATGCTTAA	chr12	64896939	64896961	+	Nanog	+	64896889	33	0.47268432	4	2:0|3:4
sgRNA_75	TACCTACCAGGATGCTTAAT	chr12	64896940	64896962	+	Nanog	+	64896889	39	0.79917607	4	2:0|3:4
sgRNA_76	AAGTGGCGATTAACTAGTGA	chr12	66276130	66276152	-	Nanog	+	66276051	56	0.61077234	4	2:0|3:4
sgRNA_77	TTAATGGGCCCCATTTAAAT	chr12	99300026	99300048	+	Nanog	+	99300057	49	0.43566321	5	2:0|3:5
sgRNA_78	CTTAATGGGCCCCATTTAAA	chr12	99300025	99300047	+	Nanog	+	99300057	15	0.44477621	3	2:1|3:2
sgRNA_79	GCTTTGCCCATTAGGATGAG	chr12	99329920	99329942	+	Nanog	-	99329998	62	0.57702058	5	2:0|3:5
sgRNA_80	TCATTTAATGGGCTACGGGC	chr13	102106349	102106371	+	Nanog	+	102106284	39	0.76732142	4	2:0|3:4
sgRNA_81	CATTTAATGGGCTACGGGCT	chr13	102106350	102106372	+	Nanog	+	102106284	38	0.89207226	3	2:0|3:3
sgRNA_82	CATTTAATGGGCTACGGGCT	chr13	102106350	102106372	+	Nanog	+	102106488	38	0.89207226	3	2:0|3:3
sgRNA_83	TCATTTAATGGGCTACGGGC	chr13	102106349	102106371	+	Nanog	+	102106488	39	0.76732142	4	2:0|3:4
sgRNA_84	AGTCTTCGGTTCAATCAAGT	chr13	111494810	111494832	-	Nanog	+	111494857	56	0.40053854	5	2:2|3:3
sgRNA_85	GCAATGCTCTGATAGTTAAT	chr13	119791615	119791637	+	Nanog	+	119791633	48	0.53947676	5	2:0|3:5
sgRNA_86	GATAGTTAATGGGTACTGAT	chr13	119791625	119791647	+	Nanog	+	119791633	60	0.64898679	4	2:0|3:4
sgRNA_87	ATAGCCTAATGGCTCTTAGC	chr13	13647485	13647507	+	Nanog	+	13647495	44	0.68509591	5	2:0|3:5
sgRNA_88	ACTGCCTAATGGCCACAGCG	chr13	22049940	22049962	-	Nanog	-	22049952	70	0.49098711	5	2:0|3:5
sgRNA_89	CCTGGGGCTCTTTTGTACCG	chr13	32613879	32613901	-	Nanog	+	32613919	62	0.53485113	5	2:1|3:4
sgRNA_90	ACCGGGCCCATTAACATTAA	chr13	38124024	38124046	+	Nanog	-	38124067	26	0.45829409	3	2:0|3:3
sgRNA_91	GCCCATTAAACACGTTAGCT	chr13	46515716	46515738	-	Nanog	+	46515747	49	0.84042021	2	2:0|3:2
sgRNA_92	ACCAAGCTAACGTGTTTAAT	chr13	46515715	46515737	+	Nanog	+	46515747	40	1.0	0	2:0|3:0
sgRNA_93	TTAAACACGTTAGCTTGGTT	chr13	46515711	46515733	-	Nanog	+	46515747	43	0.74994612	2	2:0|3:2
sgRNA_94	AACCAAGCTAACGTGTTTAA	chr13	46515714	46515736	+	Nanog	+	46515747	25	0.4773418	4	2:0|3:4
sgRNA_95	TTAATTGCCCTTTCAGGAAG	chr13	48207975	48207997	-	Nanog	-	48208016	48	0.51004512	5	2:1|3:4
sgRNA_96	CACCTAATGGCTCTCTTGGC	chr13	49420867	49420889	+	Nanog	+	49420825	49	0.4961365	5	2:0|3:5
sgRNA_97	CGGCCAAGAGAGCCATTAGG	chr13	49420866	49420888	-	Nanog	+	49420825	63	1.0	0	2:0|3:0
sgRNA_98	TGTCTGGGCCATTAGGACCC	chr13	5011861	5011883	+	Nanog	-	5011931	64	0.61837072	5	2:0|3:5
sgRNA_99	ACCAATTAACTCCAGGAGCG	chr13	52044110	52044132	-	Nanog	+	52044119	56	0.6927036	5	2:0|3:5
sgRNA_100	GCGATTAGAATACAAATGGT	chr13	52252361	52252383	+	Nanog	-	52252491	60	0.63253345	5	2:0|3:5
sgRNA_101	CAGCTTAGACGTGTGCTAAT	chr13	54199080	54199102	+	Nanog	+	54199159	41	0.7754386	1	2:0|3:1
sgRNA_102	GCAGCTTAGACGTGTGCTAA	chr13	54199079	54199101	+	Nanog	+	54199159	36	0.90706094	2	2:0|3:2
sgRNA_103	TAGAACGAAATGTTGGCTAA	chr13	58590014	58590036	-	Nanog	-	58589864	36	0.63154096	3	2:0|3:3
sgRNA_104	CAGCTTCGCGGGCAGTTAAT	chr13	6040230	6040252	-	Nanog	-	6040312	40	1.0	0	2:0|3:0
sgRNA_105	ACAGCTTCGCGGGCAGTTAA	chr13	6040231	6040253	-	Nanog	-	6040312	26	0.36292279	4	2:0|3:4
sgRNA_106	GGTTAATGGCTTTTCAGGCG	chr13	7539123	7539145	+	Nanog	+	7539100	56	0.50522244	3	2:0|3:3
sgRNA_107	TTGAAGGGCCAAACGTTAAT	chr13	84571673	84571695	+	Nanog	+	84571644	41	0.7815534	1	2:0|3:1
sgRNA_108	CTTGAAGGGCCAAACGTTAA	chr13	84571672	84571694	+	Nanog	+	84571644	27	0.88083746	3	2:0|3:3
sgRNA_109	AATAGAGGCCCATTAACGTT	chr13	84571681	84571703	-	Nanog	+	84571644	46	1.0	0	2:0|3:0
sgRNA_110	CAGCATCAAGTTGTTCTAAT	chr13	97454778	97454800	-	Nanog	-	97454887	44	0.82514808	3	2:0|3:3
sgRNA_111	TATGGCCCATTAAGTGCTAC	chr14	100097472	100097494	-	Nanog	+	100097379	46	0.9771987	1	2:0|3:1
sgRNA_112	ATGGCCCATTAAGTGCTACA	chr14	100097471	100097493	-	Nanog	+	100097379	71	0.61544903	2	2:0|3:2
sgRNA_113	AGCACTTAATGGGCCATATA	chr14	100097477	100097499	+	Nanog	+	100097379	43	0.39485439	4	2:0|3:4
sgRNA_114	GGCCTATTCTCAGGTTTAAT	chr14	122468052	122468074	-	Nanog	-	122468108	26	0.66301201	4	2:0|3:4
sgRNA_115	TTAATTGGCAGGCGGTTTAT	chr14	122468037	122468059	-	Nanog	-	122468108	32	0.81661087	2	2:0|3:2
sgRNA_116	TAATTGCCCACACCCATCGT	chr14	122468794	122468816	-	Nanog	-	122468811	62	0.73382455	2	2:0|3:2
sgRNA_117	GGGCAATTAATAAGCTCTAA	chr14	122468808	122468830	+	Nanog	-	122468811	56	0.50119983	5	2:0|3:5
sgRNA_118	CATGCCTTAATGAGCTCCGC	chr14	12338253	12338275	+	Nanog	-	12338188	53	0.53216389	4	2:0|3:4
sgRNA_119	ATTGCCAGCGGAGCTCATTA	chr14	12338257	12338279	-	Nanog	-	12338188	39	0.30475894	5	2:0|3:5
sgRNA_120	CGTTGACCTGACAGAATTAA	chr14	26557866	26557888	-	Nanog	-	26557818	23	0.69898637	4	2:0|3:4
sgRNA_121	AATTCTGTCAGGTCAACGGA	chr14	26557871	26557893	+	Nanog	-	26557818	56	0.87782627	3	2:0|3:3
sgRNA_122	AGCAACTAATGGCTACACCA	chr14	27000485	27000507	-	Nanog	-	27000530	64	0.68941715	3	2:0|3:3
sgRNA_123	TGCAAGTAATTAGCAACTAA	chr14	27000496	27000518	-	Nanog	-	27000530	37	0.56021122	5	2:0|3:5
sgRNA_124	GCAACTAATGGCTACACCAG	chr14	27000484	27000506	-	Nanog	-	27000530	67	0.67729298	5	2:1|3:4
sgRNA_125	CACGGTCGGGCCATTAAACC	chr14	29120191	29120213	+	Nanog	-	29120131	52	0.90909091	1	2:1|3:0
sgRNA_126	GGTCACCCATTAAAGCATTG	chr14	35574677	35574699	-	Nanog	+	35574725	59	0.76705634	3	2:0|3:3
sgRNA_127	ATGGGTGACCAATCATCCCC	chr14	35574690	35574712	+	Nanog	+	35574725	48	0.65188142	5	2:0|3:5
sgRNA_128	GTTATCCCAAAACGCCCATT	chr14	48652908	48652930	+	Nanog	-	48652752	50	0.81699346	1	2:0|3:1
sgRNA_129	ATGGGCGTTTTGGGATAACA	chr14	48652904	48652926	-	Nanog	-	48652752	61	0.46508773	5	2:0|3:5
sgRNA_130	TGGTGCCTAATGGGCGTTTT	chr14	48652913	48652935	-	Nanog	-	48652752	27	0.93131089	3	2:0|3:3
sgRNA_131	CTCGTGACCCCTACCCTAAT	chr14	61134378	61134400	-	Nanog	-	61134462	42	0.88311688	1	2:0|3:1
sgRNA_132	CCTCGTGACCCCTACCCTAA	chr14	61134379	61134401	-	Nanog	-	61134462	30	0.37974215	5	2:0|3:5
sgRNA_133	CCATTAGGGTAGGGGTCACG	chr14	61134379	61134401	+	Nanog	-	61134462	53	0.58963099	3	2:0|3:3
sgRNA_134	CATTAGGGTAGGGGTCACGA	chr14	61134380	61134402	+	Nanog	-	61134462	65	0.94030774	2	2:0|3:2
sgRNA_135	CTCCAGCCATTAGAGAATTC	chr14	86397097	86397119	+	Nanog	-	86397113	35	0.43396449	5	2:0|3:5
sgRNA_136	TTGCCTATTAGAAGGCGATT	chr15	101852771	101852793	-	Nanog	+	101852763	42	0.62162162	1	2:1|3:0
sgRNA_137	TTGCCTAATCGCCTTCTAAT	chr15	101852768	101852790	+	Nanog	+	101852763	34	0.72498438	2	2:0|3:2
sgRNA_138	CTAACGGGCTGCCTGACTAA	chr15	27998927	27998949	-	Nanog	-	27998952	43	0.63405964	4	2:0|3:4
sgRNA_139	GCAATTAAATCCCTACGTAG	chr15	31980081	31980103	-	Nanog	+	31980229	67	1.0	0	2:0|3:0
sgRNA_140	GGGATTTAATTGCCCCTGGC	chr15	31980091	31980113	+	Nanog	+	31980229	46	0.48821423	4	2:0|3:4
sgRNA_141	CGTAGGGATTTAATTGCCCC	chr15	31980087	31980109	+	Nanog	+	31980229	51	0.79782412	2	2:0|3:2
sgRNA_142	ATTAACTCGCCTCCATCATG	chr15	4439533	4439555	-	Nanog	+	4439449	62	0.70453545	2	2:0|3:2
sgRNA_143	TCATGATGGAGGCGAGTTAA	chr15	4439535	4439557	+	Nanog	+	4439449	28	0.57934467	5	2:0|3:5
sgRNA_144	CATGATGGAGGCGAGTTAAT	chr15	4439536	4439558	+	Nanog	+	4439449	40	0.72571774	2	2:0|3:2
sgRNA_145	GACATGGCCATTAGTAACCA	chr15	57824477	57824499	-	Nanog	+	57824510	64	0.64883599	4	2:1|3:3
sgRNA_146	GAGGGCTGTGGCGGGCCATT	chr15	59110238	59110260	+	Nanog	-	59110083	21	0.6634432	5	2:0|3:5
sgRNA_147	AGGGCTGTGGCGGGCCATTA	chr15	59110239	59110261	+	Nanog	-	59110083	34	0.57746683	5	2:0|3:5
sgRNA_148	ACCTATTAATTGCCCTCCGG	chr15	7281437	7281459	-	Nanog	-	7281475	56	0.98684211	1	2:0|3:1
sgRNA_149	GATACCTATTAATTGCCCTC	chr15	7281440	7281462	-	Nanog	-	7281475	56	0.67124189	2	2:0|3:2
sgRNA_150	GCCTCCGGAGGGCAATTAAT	chr15	7281436	7281458	+	Nanog	-	7281475	35	0.66301068	3	2:0|3:3
sgRNA_151	GGGGTTTGCTGGTACACTAA	chr15	77202181	77202203	-	Nanog	-	77202336	38	0.61434449	4	2:0|3:4
sgRNA_152	TGCTAATGGGTGCATGTACT	chr16	10300776	10300798	-	Nanog	-	10300637	53	0.68267735	4	2:0|3:4
sgRNA_153	GCAAGTCCTAACTTTTTAAT	chr16	17186732	17186754	+	Nanog	+	17186716	32	0.48665115	5	2:0|3:5
sgRNA_154	AAGGCGATTAATCCCGTCTA	chr16	22409026	22409048	+	Nanog	-	22409189	42	1.0	1	2:0|3:1
sgRNA_155	TTCCGCTGAGGGACCTTAAT	chr16	22413113	22413135	-	Nanog	-	22413094	34	0.6093691	2	2:0|3:2
sgRNA_156	AACCGATTAAGGTCCCTCAG	chr16	22413111	22413133	+	Nanog	-	22413094	73	0.41481481	4	2:1|3:3
sgRNA_157	AGCTGGTGGCACGAGTCTAA	chr16	23103026	23103048	-	Nanog	-	23102999	45	0.84420821	3	2:0|3:3
sgRNA_158	TTGCCTATCCCAAGATTAAT	chr16	25038985	25039007	-	Nanog	-	25038847	24	0.71017544	2	2:0|3:2
sgRNA_159	TTAATCTTGGGATAGGCAAT	chr16	25038989	25039011	+	Nanog	-	25038847	53	0.43659642	5	2:0|3:5
sgRNA_160	AGTGCAGCCGGAGAGCTAAT	chr16	31546326	31546348	+	Nanog	+	31546425	47	0.77103815	3	2:0|3:3
sgRNA_161	CCTTGTTTCTCGGGCGTTCC	chr16	34956748	34956770	+	Nanog	-	34956817	14	0.89810017	3	2:0|3:3
sgRNA_162	TTGAGTCATCCGACCCTTAA	chr16	38305054	38305076	-	Nanog	-	38305020	21	0.54855398	2	2:0|3:2
sgRNA_163	ACTCAGAATGGTGGCCATTA	chr16	38305040	38305062	+	Nanog	-	38305020	26	0.74411924	5	2:0|3:5
sgRNA_164	TCAAGCCCGCTGAAGCCTAA	chr16	43310254	43310276	+	Nanog	+	43310273	45	0.82935638	2	2:0|3:2
sgRNA_165	CTGCGTTTCACTTTAATCGT	chr16	70314736	70314758	+	Nanog	+	70314747	47	0.80620155	1	2:0|3:1
sgRNA_166	GAGATTAATGGCTCAGCGTG	chr16	75616534	75616556	-	Nanog	-	75616410	61	0.55147961	5	2:0|3:5
sgRNA_167	GAGCCATTAAAGGATTAACT	chr16	8545588	8545610	-	Nanog	+	8545600	66	0.71162839	5	2:0|3:5
sgRNA_168	TCAGAAAACGATTAAGGCTC	chr16	90902954	90902976	+	Nanog	-	90902991	50	0.53393566	5	2:0|3:5
sgRNA_169	CCGGACTTCAGACTTTTAAT	chr16	91565164	91565186	-	Nanog	-	91565104	23	0.47082289	4	2:0|3:4
sgRNA_170	CGGCTTTAATTGGTGCAGAG	chr17	13668795	13668817	-	Nanog	-	13668749	61	0.65320197	1	2:0|3:1
sgRNA_171	GCATCAAATTCGGCTTTAAT	chr17	13668805	13668827	-	Nanog	-	13668749	28	0.93034826	1	2:0|3:1
sgRNA_172	TTAGGTTACCACAGTCGGCA	chr17	15247906	15247928	+	Nanog	-	15247867	61	0.58625321	4	2:0|3:4
sgRNA_173	GGTAACCTAATGGCTGCCAG	chr17	15247893	15247915	-	Nanog	-	15247867	70	0.8331791	4	2:0|3:4
sgRNA_174	TGCCGACTGTGGTAACCTAA	chr17	15247903	15247925	-	Nanog	-	15247867	48	1.0	0	2:0|3:0
sgRNA_175	TTAGGTTACCACAGTCGGCA	chr17	15247906	15247928	+	Nanog	-	15248066	61	0.58625321	4	2:0|3:4
sgRNA_176	TGCCGACTGTGGTAACCTAA	chr17	15247903	15247925	-	Nanog	-	15248066	48	1.0	0	2:0|3:0
sgRNA_177	GGTAACCTAATGGCTGCCAG	chr17	15247893	15247915	-	Nanog	-	15248066	70	0.8331791	4	2:0|3:4
sgRNA_178	AATGTTATGGCTTTAATCGT	chr17	17408256	17408278	+	Nanog	+	17408338	56	0.51405613	3	2:0|3:3
sgRNA_179	TTATGGCTTTAATCGTTGGT	chr17	17408260	17408282	+	Nanog	+	17408338	50	0.74618707	4	2:0|3:4
sgRNA_180	TAATGGGGCTGATGCGTTAA	chr17	27179822	27179844	+	Nanog	+	27179879	33	0.98734177	1	2:0|3:1
sgRNA_181	GGGTCGAGCAAACTACTTAA	chr17	27994476	27994498	+	Nanog	+	27994535	34	0.54321941	5	2:0|3:5
sgRNA_182	ACTCCCGCAAAGAAGCCATT	chr17	29213577	29213599	-	Nanog	+	29213599	39	0.53146368	3	2:0|3:3
sgRNA_183	GAACAAAGCGGGCCATTAAA	chr17	31766964	31766986	-	Nanog	+	31767057	32	0.62397296	5	2:0|3:5
sgRNA_184	TGTCACGGTTTAATTGCTTA	chr17	35708282	35708304	+	Nanog	+	35708231	39	0.54138399	3	2:0|3:3
sgRNA_185	TAATGGGTCTTGTTAAGACC	chr17	42037308	42037330	+	Nanog	+	42037322	56	0.61149387	3	2:0|3:3
sgRNA_186	GTCTTAATTGCTTTCGTAAG	chr17	62758587	62758609	-	Nanog	-	62758558	37	0.71407553	4	2:0|3:4
sgRNA_187	TTGGCCATTAAGACGCTCCA	chr17	66190768	66190790	-	Nanog	+	66190783	55	0.47009849	5	2:0|3:5
sgRNA_188	TTGGAGCGTCTTAATGGCCA	chr17	66190770	66190792	+	Nanog	+	66190783	56	0.44762906	5	2:1|3:4
sgRNA_189	CAGTGGCCCATTAAGTCTTT	chr17	70515044	70515066	-	Nanog	+	70515090	30	0.61809582	3	2:0|3:3
sgRNA_190	GGGTCTTACTGATTGGCCAG	chr17	75233290	75233312	+	Nanog	+	75233372	64	0.73807109	5	2:0|3:5
sgRNA_191	TTAGCGCCCACTCTGCTTAA	chr17	8389219	8389241	+	Nanog	+	8389263	26	0.78640777	1	2:0|3:1
sgRNA_192	ATGCCTAGGAATTTAGCTAA	chr17	85149419	85149441	+	Nanog	+	85149410	49	0.46893622	5	2:0|3:5
sgRNA_193	TTAGTCGCTCAGCGTGGGCT	chr17	85487060	85487082	-	Nanog	+	85487102	33	0.60311447	4	2:0|3:4
sgRNA_194	AGCGATTAGTCGCTCAGCGT	chr17	85487065	85487087	-	Nanog	+	85487102	54	0.84848485	1	2:0|3:1
sgRNA_195	AAGCGATTAGTCGCTCAGCG	chr17	85487066	85487088	-	Nanog	+	85487102	60	1.0	0	2:0|3:0
sgRNA_196	ATTAGTCGCTCAGCGTGGGC	chr17	85487061	85487083	-	Nanog	+	85487102	36	0.90502117	2	2:0|3:2
sgRNA_197	ACGCTGAGCGACTAATCGCT	chr17	85487068	85487090	+	Nanog	+	85487102	54	0.88135593	1	2:0|3:1
sgRNA_198	CTAATCGCTTGGCCATTGTC	chr17	85487079	85487101	+	Nanog	+	85487102	39	0.402746	3	2:1|3:2
sgRNA_199	ATGGCTAACGGCCATTAATT	chr17	85935338	85935360	-	Nanog	+	85935503	17	0.81225069	2	2:0|3:2
sgRNA_200	GGCTAACGGCCATTAATTTG	chr17	85935336	85935358	-	Nanog	+	85935503	56	0.77473958	2	2:0|3:2
sgRNA_201	TGGCTAACGGCCATTAATTT	chr17	85935337	85935359	-	Nanog	+	85935503	27	0.89446044	2	2:0|3:2
sgRNA_202	GCTACTTCCACTAGCTCATG	chr17	87445840	87445862	+	Nanog	+	87445789	56	0.54397582	5	2:1|3:4
sgRNA_203	TGGGCGATTAACAAGCTCTG	chr17	88258619	88258641	+	Nanog	-	88258618	74	0.40396275	5	2:0|3:5
sgRNA_204	CGCCCATTACCAGCAGCGGC	chr17	88258602	88258624	-	Nanog	-	88258618	49	0.70170612	3	2:0|3:3
sgRNA_205	AGCCGGCCGCTGCTGGTAAT	chr17	88258600	88258622	+	Nanog	-	88258618	40	0.53405032	5	2:1|3:4
sgRNA_206	TAATCGCCCATTACCAGCAG	chr17	88258606	88258628	-	Nanog	-	88258618	62	0.58874071	2	2:0|3:2
sgRNA_207	GGTGATCTAATACTGCTTAA	chr18	31122413	31122435	+	Nanog	+	31122530	24	0.44644979	4	2:0|3:4
sgRNA_208	CTGTCATTGTCTAATGGGCC	chr18	39067989	39068011	+	Nanog	+	39067998	36	0.37249178	5	2:1|3:4
sgRNA_209	GGGCTGTTAATCGCTCTGAC	chr18	47703898	47703920	-	Nanog	-	47703907	56	0.86206434	3	2:0|3:3
sgRNA_210	GCGGGTCAGAGCGATTAGTC	chr18	47703879	47703901	+	Nanog	-	47703907	59	0.98615917	1	2:0|3:1
sgRNA_211	CGTCTAACCCATTAAGGAGC	chr18	75449786	75449808	-	Nanog	+	75449922	53	0.58762192	2	2:0|3:2
sgRNA_212	TAATGGGTTAGACGTAAGCA	chr18	75449795	75449817	+	Nanog	+	75449922	64	0.43266075	5	2:1|3:4
sgRNA_213	TAACCCATTAAGGAGCCGGC	chr18	75449782	75449804	-	Nanog	+	75449922	50	0.83395107	1	2:0|3:1
sgRNA_214	TGCTTACGTCTAACCCATTA	chr18	75449792	75449814	-	Nanog	+	75449922	27	1.0	0	2:0|3:0
sgRNA_215	ACCTTTTGTCATGCGGGAGT	chr18	75579572	75579594	+	Nanog	-	75579556	42	0.72484366	1	2:0|3:1
sgRNA_216	ACATGTCCGAGACTCCCTAA	chr18	75579595	75579617	-	Nanog	-	75579556	49	0.83870968	1	2:0|3:1
sgRNA_217	GCCCACTCCCGCATGACAAA	chr18	75579573	75579595	-	Nanog	-	75579556	47	0.84	2	2:1|3:1
sgRNA_218	CATGCGGGAGTGGGCCATTA	chr18	75579581	75579603	+	Nanog	-	75579556	36	0.90322581	1	2:0|3:1
sgRNA_219	TCATGCGGGAGTGGGCCATT	chr18	75579580	75579602	+	Nanog	-	75579556	37	0.69764677	3	2:1|3:2
sgRNA_220	GAGTACTTAGGCAATTAAGT	chr19	10557518	10557540	-	Nanog	+	10557617	54	0.6075691	4	2:0|3:4
sgRNA_221	GGCCTTGATATTGAGTACTT	chr19	10557530	10557552	-	Nanog	+	10557617	54	0.39727964	5	2:0|3:5
sgRNA_222	ACCACTTAATGGCTCCGTCC	chr19	10719846	10719868	+	Nanog	+	10719974	49	1.0	0	2:0|3:0
sgRNA_223	GAAGCCATTAAGTGCTCCAA	chr19	16419312	16419334	-	Nanog	+	16419208	56	0.35184598	5	2:0|3:5
sgRNA_224	ACATCCTTTGGAGCACTTAA	chr19	16419308	16419330	+	Nanog	+	16419208	22	0.47893411	5	2:0|3:5
sgRNA_225	GAGAAATTAATGGGTACTCA	chr19	23153928	23153950	-	Nanog	-	23153927	56	0.53500574	5	2:0|3:5
sgRNA_226	ATTAACAGCTAACTTCGTAT	chr19	26930722	26930744	-	Nanog	+	26930769	64	0.54234913	5	2:1|3:4
sgRNA_227	TAAGCAATTAAGACTTCACA	chr19	46435724	46435746	-	Nanog	+	46435699	62	0.53707725	5	2:1|3:4
sgRNA_228	CGATTAGCAACAGAGGACAA	chr19	48771766	48771788	+	Nanog	-	48771768	65	0.29964385	5	2:1|3:4
sgRNA_229	TGGAAACCGATTAGCAACAG	chr19	48771759	48771781	+	Nanog	-	48771768	69	0.52645958	5	2:0|3:5
sgRNA_230	TTATGTTAAGCGCCCATTAA	chr19	50475538	50475560	-	Nanog	+	50475483	23	0.62817845	3	2:0|3:3
sgRNA_231	AATCGTCCTATAATCCCTGC	chr19	50656928	50656950	-	Nanog	-	50657003	56	0.75890862	3	2:0|3:3
sgRNA_232	GGACGATTAACAAAGAAGGT	chr19	50656943	50656965	+	Nanog	-	50657003	64	0.34470283	5	2:0|3:5
sgRNA_233	TATAGGACGATTAACAAAGA	chr19	50656939	50656961	+	Nanog	-	50657003	56	0.7250534	3	2:0|3:3
sgRNA_234	CATTAGGACTCTTAACTAAT	chr1	104987833	104987855	+	Nanog	+	104987698	44	0.5909162	4	2:0|3:4
sgRNA_235	TAATGGGTGGGATCCCATGC	chr1	104987849	104987871	+	Nanog	+	104987698	53	0.5171436	4	2:0|3:4
sgRNA_236	TAGGACTCTTAACTAATGGG	chr1	104987836	104987858	+	Nanog	+	104987698	59	0.81775701	3	2:0|3:3
sgRNA_237	GCCTATAGCAAAGGCGGATA	chr1	11082195	11082217	-	Nanog	-	11082264	37	0.69672619	5	2:0|3:5
sgRNA_238	TCCTTATCCGCCTTTGCTAT	chr1	11082194	11082216	+	Nanog	-	11082264	40	0.64147363	5	2:0|3:5
sgRNA_239	TTGGTCAGAAGCCATTAGTC	chr1	132282093	132282115	-	Nanog	+	132282058	51	0.45963324	5	2:0|3:5
sgRNA_240	AGTGGTCACCGAAGAGATTG	chr1	134941211	134941233	-	Nanog	+	134941187	59	0.9384858	1	2:0|3:1
sgRNA_241	AGTGGTCACCGAAGAGATTG	chr1	134941211	134941233	-	Nanog	+	134941386	59	0.9384858	1	2:0|3:1
sgRNA_242	TTAATGGCCATGACGCGGAT	chr1	151038706	151038728	-	Nanog	-	151038746	53	0.94324411	1	2:0|3:1
sgRNA_243	TGGACTTAATGGCCATGACG	chr1	151038711	151038733	-	Nanog	-	151038746	63	0.78841517	3	2:0|3:3
sgRNA_244	CTGTCAACCAATCCGCGTCA	chr1	151038699	151038721	+	Nanog	-	151038746	56	1.0	0	2:0|3:0
sgRNA_245	CATCACCTCCTGGTGCTTAA	chr1	153316445	153316467	+	Nanog	+	153316455	24	0.64238169	5	2:0|3:5
sgRNA_246	GTGCCCATTAGGAAACTCAA	chr1	155474311	155474333	-	Nanog	+	155474458	63	0.79035935	2	2:0|3:2
sgRNA_247	AATCGCCGGGCCATCACACC	chr1	156718379	156718401	+	Nanog	+	156718355	54	0.51422786	3	2:0|3:3
sgRNA_248	TGGGCTCGCTCTTAATCGCC	chr1	156718366	156718388	+	Nanog	+	156718355	47	1.0	0	2:0|3:0
sgRNA_249	CATTTCCGGGTGTGATGGCC	chr1	156718384	156718406	-	Nanog	+	156718355	38	0.41780228	5	2:0|3:5
sgRNA_250	CTGGGCTCGCTCTTAATCGC	chr1	156718365	156718387	+	Nanog	+	156718355	43	0.75675676	3	2:0|3:3
sgRNA_251	TCTAATGGCTCTTGTCCGAA	chr1	165178574	165178596	+	Nanog	+	165178747	56	0.68526093	4	2:0|3:4
sgRNA_252	TTAGCATGACTGTCGGCTTT	chr1	166300708	166300730	-	Nanog	+	166300736	24	0.97540984	1	2:0|3:1
sgRNA_253	ACAATGACGCCCGCAGTTAA	chr1	176613058	176613080	-	Nanog	-	176613098	23	0.7202545	3	2:0|3:3
sgRNA_254	CCATCAAGGGCAATTAACCA	chr1	187439704	187439726	+	Nanog	-	187439697	69	0.69865378	2	2:0|3:2
sgRNA_255	CCGTGGTTAATTGCCCTTGA	chr1	187439704	187439726	-	Nanog	-	187439697	30	0.6115916	3	2:0|3:3
sgRNA_256	CAATTAACCACGGAGAACTC	chr1	187439714	187439736	+	Nanog	-	187439697	59	0.60567648	4	2:0|3:4
sgRNA_257	GATGGGCCGCATTCTTTAAT	chr1	78168098	78168120	+	Nanog	+	78168147	19	0.37428322	4	2:0|3:4
sgRNA_258	GCTGGTCTTGAGAGCCCATT	chr1	80622951	80622973	+	Nanog	-	80622962	33	0.6286857	4	2:0|3:4
sgRNA_259	TGTGAATGTAGACCCCTAAT	chr1	80622965	80622987	-	Nanog	-	80622962	47	0.59837246	4	2:0|3:4
sgRNA_260	TGACAAAGGTTTGATCTAAT	chr1	89751140	89751162	+	Nanog	+	89751225	46	0.4996562	5	2:0|3:5
sgRNA_261	GGCTGTCATTCACGTAGTCT	chr2	10521979	10522001	+	Nanog	+	10521898	55	0.96491228	2	2:1|3:1
sgRNA_262	CTGTTTGACTCCTGATCCCG	chr2	109579163	109579185	+	Nanog	+	109579117	60	0.5418168	5	2:0|3:5
sgRNA_263	GTTGGTTAATCTAGCAATTA	chr2	109936408	109936430	-	Nanog	+	109936555	22	0.66647606	5	2:1|3:4
sgRNA_264	TTGGGCCATTAGTTGAGGTT	chr2	115685500	115685522	+	Nanog	-	115685435	42	0.69524558	5	2:1|3:4
sgRNA_265	TGAGCAATTAACTTACCAGC	chr2	11767106	11767128	+	Nanog	-	11767094	61	0.55552167	4	2:1|3:3
sgRNA_266	AACTAAATGCACGATGATTG	chr2	132474572	132474594	-	Nanog	+	132474611	53	0.50759911	3	2:0|3:3
sgRNA_267	AACTATCCGAATTAATTGCC	chr2	154925598	154925620	-	Nanog	-	154925592	48	0.76726343	1	2:0|3:1
sgRNA_268	GAAACGTTTTCACTTACTAA	chr2	157968096	157968118	-	Nanog	-	157968076	31	0.63212369	5	2:0|3:5
sgRNA_269	TGCCTGCTAATGGCTCGCCA	chr2	166169064	166169086	-	Nanog	-	166169074	50	0.41593096	5	2:0|3:5
sgRNA_270	GCCTGCTAATGGCTCGCCAC	chr2	166169063	166169085	-	Nanog	-	166169074	47	0.88888889	1	2:0|3:1
sgRNA_271	GCTAATGGCTCGCCACGGGT	chr2	166169059	166169081	-	Nanog	-	166169074	52	0.79783394	2	2:0|3:2
sgRNA_272	ACCCGTGGCGAGCCATTAGC	chr2	166169062	166169084	+	Nanog	-	166169074	44	0.66307975	2	2:0|3:2
sgRNA_273	TGCTAATGGCTCGCCACGGG	chr2	166169060	166169082	-	Nanog	-	166169074	39	0.90592334	1	2:0|3:1
sgRNA_274	GAGGCTTACTGTGAGACTAA	chr2	166891594	166891616	+	Nanog	+	166891672	51	0.76901048	5	2:0|3:5
sgRNA_275	ACACAATAGAATTTAATCGC	chr2	169311871	169311893	-	Nanog	-	169311837	64	0.70858439	4	2:0|3:4
sgRNA_276	GCTTCTTTATGATGGCGATT	chr2	170775806	170775828	+	Nanog	-	170775816	27	0.53162402	4	2:0|3:4
sgRNA_277	GCATTAATCGTTTGCTTAAT	chr2	174288226	174288248	+	Nanog	+	174288080	25	0.46558483	4	2:0|3:4
sgRNA_278	CCCTGCCCTTAATGGCTTAC	chr2	178135709	178135731	-	Nanog	-	178135881	9	0.60275967	3	2:0|3:3
sgRNA_279	CCAGTAAGCCATTAAGGGCA	chr2	178135709	178135731	+	Nanog	-	178135881	56	0.69304812	4	2:0|3:4
sgRNA_280	TACTCAGGGCAATTAACGGC	chr2	18686992	18687014	-	Nanog	+	18687047	52	0.83820168	1	2:0|3:1
sgRNA_281	GTTAATTGCCCTGAGTAGAA	chr2	18686998	18687020	+	Nanog	+	18687047	51	0.47786454	4	2:0|3:4
sgRNA_282	GGGCAACCCCTTTCTACTCA	chr2	18687006	18687028	-	Nanog	+	18687047	61	1.0	3	2:0|3:3
sgRNA_283	CAATTAACGGCCGGCAATTT	chr2	18686983	18687005	-	Nanog	+	18687047	21	1.0	0	2:0|3:0
sgRNA_284	TTAACGGCCGGCAATTTAGG	chr2	18686980	18687002	-	Nanog	+	18687047	56	0.74168954	1	2:0|3:1
sgRNA_285	GGCAATTAATGGTCCCCTGA	chr2	19383433	19383455	-	Nanog	+	19383290	55	0.30241842	5	2:1|3:4
sgRNA_286	AATTGGCTTCAGTACAGGGC	chr2	26500897	26500919	+	Nanog	+	26500983	54	0.4133175	5	2:0|3:5
sgRNA_287	TGTACTGAAGCCAATTAAGC	chr2	26500890	26500912	-	Nanog	+	26500983	56	0.51562746	5	2:0|3:5
sgRNA_288	TAATTGGCTTCAGTACAGGG	chr2	26500896	26500918	+	Nanog	+	26500983	67	0.55418536	5	2:0|3:5
sgRNA_289	TGACTGCGGGCGATTAGCGC	chr2	33580852	33580874	-	Nanog	+	33580834	47	1.0	0	2:0|3:0
sgRNA_290	GTTCAGTTTAATGGCCAAAG	chr2	34166742	34166764	-	Nanog	-	34166736	56	0.45771674	5	2:1|3:4
sgRNA_291	GTGCTCAATAGATACCACTT	chr2	34166728	34166750	+	Nanog	-	34166736	61	0.56132172	5	2:0|3:5
sgRNA_292	ATCACTACTTATCAGCGATT	chr2	50556759	50556781	-	Nanog	+	50556819	47	0.43731778	3	2:0|3:3
sgRNA_293	CTAGCGGGCGATTAGTGATC	chr2	5373128	5373150	+	Nanog	-	5373018	43	0.53543307	2	2:0|3:2
sgRNA_294	TTAATGGCCCGTTTCCTGCT	chr2	70568791	70568813	+	Nanog	+	70568814	40	0.66523774	3	2:0|3:3
sgRNA_295	AGGAAACGGGCCATTAAGCC	chr2	70568785	70568807	-	Nanog	+	70568814	48	0.74017207	4	2:0|3:4
sgRNA_296	ACGGGCCATTAAGCCAGGAA	chr2	70568780	70568802	-	Nanog	+	70568814	60	0.46664192	4	2:0|3:4
sgRNA_297	CAGCAAAGCGGTGGGTTAAT	chr2	71243214	71243236	-	Nanog	-	71243244	37	0.4297947	5	2:0|3:5
sgRNA_298	CTAATGGCTTATTAATAAGA	chr2	75048447	75048469	-	Nanog	-	75048300	49	0.36790296	5	2:1|3:4
sgRNA_299	CTAATGGCTTATTAATAAGA	chr2	75048447	75048469	-	Nanog	-	75048500	49	0.36790296	5	2:1|3:4
sgRNA_300	GCCCGACCATCCTGTGCTAA	chr2	75538331	75538353	+	Nanog	+	75538386	42	0.87640449	1	2:0|3:1
sgRNA_301	GCTCACGTTGCTACTCCAGT	chr2	75538353	75538375	+	Nanog	+	75538386	55	0.91724138	1	2:0|3:1
sgRNA_302	CTCACGTTGCTACTCCAGTG	chr2	75538354	75538376	+	Nanog	+	75538386	70	0.82486522	2	2:0|3:2
sgRNA_303	GCCATTAGCACAGGATGGTC	chr2	75538332	75538354	-	Nanog	+	75538386	49	0.65770751	3	2:0|3:3
sgRNA_304	AGTTGAAGTGAGGGCGATTA	chr2	96315160	96315182	-	Nanog	+	96315255	33	0.96486486	3	2:0|3:3
sgRNA_305	CCGTTTAAGCAACAGCCATT	chr3	102055264	102055286	-	Nanog	+	102055297	37	0.66616362	3	2:0|3:3
sgRNA_306	ATCTTGGTGAGAGGACCTAA	chr3	102055249	102055271	+	Nanog	+	102055297	44	0.41123622	5	2:0|3:5
sgRNA_307	GTTAATCGTTGGTTCTCTCC	chr3	103683404	103683426	-	Nanog	-	103683480	51	0.6187175	3	2:0|3:3
sgRNA_308	TCTATTCAAACGCTAATCGG	chr3	118626533	118626555	+	Nanog	+	118626624	59	0.76159148	2	2:0|3:2
sgRNA_309	CTATTCAAACGCTAATCGGT	chr3	118626534	118626556	+	Nanog	+	118626624	60	1.0	0	2:0|3:0
sgRNA_310	TTAAACTAGGCTAATGGCCT	chr3	142334924	142334946	+	Nanog	+	142334965	48	0.67314716	4	2:0|3:4
sgRNA_311	TGCTAATCGCCCCCTTCAAA	chr3	148986472	148986494	+	Nanog	+	148986449	48	0.6200776	4	2:0|3:4
sgRNA_312	GGTTAATGGGCATCGCCACC	chr3	34559438	34559460	+	Nanog	+	34559294	56	0.59602718	3	2:0|3:3
sgRNA_313	GCGATGCCCATTAACCTTTC	chr3	34559431	34559453	-	Nanog	+	34559294	21	0.82833133	2	2:0|3:2
sgRNA_314	TAATCGCCTAATAAATGTAT	chr3	34735302	34735324	-	Nanog	-	34735204	54	0.78519847	2	2:0|3:2
sgRNA_315	TTAATCGCCTAATAAATGTA	chr3	34735303	34735325	-	Nanog	-	34735204	43	0.65976143	3	2:0|3:3
sgRNA_316	GCCATTAAATCGGAGATCAG	chr3	47891117	47891139	+	Nanog	-	47891057	65	0.53692115	4	2:0|3:4
sgRNA_317	ATGGGCCACCAAGGTTTATC	chr3	68301694	68301716	-	Nanog	-	68301744	28	0.58310924	5	2:0|3:5
sgRNA_318	GGCTTCTAATGGCTTATGAG	chr3	68894553	68894575	-	Nanog	-	68894651	56	0.72912447	5	2:0|3:5
sgRNA_319	ATCCGACTGTGAGCCATTAG	chr3	79385573	79385595	+	Nanog	-	79385546	52	1.0	1	2:0|3:1
sgRNA_320	TGGGTCGATTACTTATCATT	chr3	87137037	87137059	-	Nanog	-	87137040	42	0.59823394	4	2:0|3:4
sgRNA_321	TAAGTAATCGACCCATTAGG	chr3	87137045	87137067	+	Nanog	-	87137040	63	0.84210526	1	2:0|3:1
sgRNA_322	ACCATGGCCGGATGACCCTC	chr3	88646663	88646685	-	Nanog	+	88646662	48	0.38752821	4	2:0|3:4
sgRNA_323	GCCTGAGGGTCATCCGGCCA	chr3	88646662	88646684	+	Nanog	+	88646662	50	0.62038405	2	2:0|3:2
sgRNA_324	ATGGTGACTTAATTGCCTGA	chr3	88646648	88646670	+	Nanog	+	88646662	62	0.72376797	4	2:0|3:4
sgRNA_325	ATCGCCCAATTACTTGCTCA	chr3	89998249	89998271	+	Nanog	+	89998383	56	0.64863827	3	2:0|3:3
sgRNA_326	TTGGGCGATTAGTAGTAGGC	chr3	89998235	89998257	-	Nanog	+	89998383	56	0.79293059	3	2:0|3:3
sgRNA_327	GTAATTGGGCGATTAGTAGT	chr3	89998239	89998261	-	Nanog	+	89998383	51	0.76359338	2	2:0|3:2
sgRNA_328	TCTGCATTAATCGACACTAA	chr4	105658690	105658712	-	Nanog	-	105658700	39	0.45510735	3	2:0|3:3
sgRNA_329	CAAGCTAATCGGCCATAGCA	chr4	118517681	118517703	+	Nanog	+	118517817	59	0.80685914	2	2:0|3:2
sgRNA_330	ATGGCCGATTAGCTTGACAA	chr4	118517674	118517696	-	Nanog	+	118517817	69	0.81472684	2	2:0|3:2
sgRNA_331	AATTAACCAGCCAGGCCCCG	chr4	125534646	125534668	+	Nanog	-	125534702	69	0.61754386	3	2:0|3:3
sgRNA_332	GGGGGGCGAGCAATTAAAAT	chr4	136593993	136594015	-	Nanog	+	136594159	38	0.86764206	2	2:0|3:2
sgRNA_333	ACCCCTGCATGTAGCTTAAT	chr4	140160466	140160488	+	Nanog	+	140160357	36	0.38010216	5	2:0|3:5
sgRNA_334	GGACCCATTAAGCTACATGC	chr4	140160469	140160491	-	Nanog	+	140160357	51	0.68920005	3	2:0|3:3
sgRNA_335	ATTGCCCATTAACTCTTCCC	chr4	144894916	144894938	+	Nanog	-	144894916	53	0.71460138	3	2:0|3:3
sgRNA_336	TCTATTCACCTCTAATGGGC	chr4	150241745	150241767	+	Nanog	+	150241926	53	0.58968795	4	2:0|3:4
sgRNA_337	ATTAAGGAGTGGTACTCAGC	chr4	39695294	39695316	-	Nanog	+	39695410	54	0.56362646	3	2:0|3:3
sgRNA_338	AGCCACCACTGAAGCAATTA	chr4	39695310	39695332	-	Nanog	+	39695410	31	0.48286488	4	2:1|3:3
sgRNA_339	TAGATATTCCATTAATTGCC	chr4	40713912	40713934	+	Nanog	+	40713894	45	0.62571097	4	2:0|3:4
sgRNA_340	TGAAGCCATTAAAGACCCGG	chr4	54987267	54987289	+	Nanog	-	54987310	72	0.61137942	4	2:0|3:4
sgRNA_341	CTCTACCTCCGGGTCTTTAA	chr4	54987272	54987294	-	Nanog	-	54987310	22	0.41044524	4	2:0|3:4
sgRNA_342	ATTAGGCTGTGCCCTTGCGG	chr4	5736833	5736855	+	Nanog	-	5736814	55	0.56977267	4	2:0|3:4
sgRNA_343	ACCGCAAGGGCACAGCCTAA	chr4	5736831	5736853	-	Nanog	-	5736814	42	0.75061647	3	2:0|3:3
sgRNA_344	GGGCCATTAATTGAAGACAA	chr4	6043993	6044015	+	Nanog	-	6043905	56	0.42346815	5	2:0|3:5
sgRNA_345	CTTAATTGCTTGTGACGGCT	chr4	71765353	71765375	+	Nanog	+	71765336	46	0.55110295	2	2:0|3:2
sgRNA_346	TCTTAATTGCTTGTGACGGC	chr4	71765352	71765374	+	Nanog	+	71765336	27	0.56269459	4	2:1|3:3
sgRNA_347	ACCCATTAAACCCATAGATG	chr4	8179066	8179088	+	Nanog	-	8179174	56	0.41503833	5	2:0|3:5
sgRNA_348	AGTCAATAGGTGGCGTTAAT	chr4	84232477	84232499	-	Nanog	-	84232548	36	0.91991786	1	2:0|3:1
sgRNA_349	ATGGCTCGTCACTCCTGCAC	chr4	87834462	87834484	-	Nanog	-	87834457	55	0.82114175	2	2:0|3:2
sgRNA_350	ACTTAGTAACGCAGCTTAAT	chr5	122549217	122549239	-	Nanog	-	122549162	32	0.82644628	3	2:0|3:3
sgRNA_351	CACTTAGTAACGCAGCTTAA	chr5	122549218	122549240	-	Nanog	-	122549162	24	0.95454545	1	2:0|3:1
sgRNA_352	GCACATGGCGATTAGGAAGT	chr5	127477525	127477547	-	Nanog	+	127477693	56	0.59027377	4	2:0|3:4
sgRNA_353	GATACTTAATTGCCCTTAAT	chr5	128291357	128291379	+	Nanog	+	128291331	28	0.80788759	3	2:0|3:3
sgRNA_354	AAGGATAAGATAGCCCATTA	chr5	128291370	128291392	-	Nanog	+	128291331	31	0.58338252	4	2:0|3:4
sgRNA_355	CTCTTAGTAAAGACCTTAAT	chr5	131851992	131852014	-	Nanog	-	131852024	30	0.58962501	5	2:0|3:5
sgRNA_356	GATTTCACGGCCAATTAAGC	chr5	142179466	142179488	-	Nanog	+	142179463	42	0.83056573	2	2:0|3:2
sgRNA_357	AGCAAACCATCCGGACTAAT	chr5	142415569	142415591	+	Nanog	+	142415585	41	0.5959313	4	2:0|3:4
sgRNA_358	TCCCTGATGGCCCATTAGTC	chr5	142415579	142415601	-	Nanog	+	142415585	42	0.78389663	3	2:0|3:3
sgRNA_359	GAGCAAACCATCCGGACTAA	chr5	142415568	142415590	+	Nanog	+	142415585	38	0.71428571	2	2:1|3:1
sgRNA_360	TCCGGACTAATGGGCCATCA	chr5	142415578	142415600	+	Nanog	+	142415585	60	0.82352941	1	2:0|3:1
sgRNA_361	ATCCGGACTAATGGGCCATC	chr5	142415577	142415599	+	Nanog	+	142415585	38	0.62519042	3	2:0|3:3
sgRNA_362	TGATGGCCCATTAGTCCGGA	chr5	142415575	142415597	-	Nanog	+	142415585	55	0.50498891	3	2:0|3:3
sgRNA_363	AGGTAGCGTTCAGTATTAAT	chr5	142808007	142808029	+	Nanog	+	142807930	32	0.80473373	1	2:0|3:1
sgRNA_364	CCAAGCATCAAATGCTTAAT	chr5	143346615	143346637	-	Nanog	-	143346636	37	0.53364587	5	2:1|3:4
sgRNA_365	TAGCGGCGGTCCTACTGTCT	chr5	147895610	147895632	-	Nanog	+	147895536	45	0.79545455	1	2:0|3:1
sgRNA_366	GCGTTGGCGGCCATTAGCGG	chr5	147895624	147895646	-	Nanog	+	147895536	63	0.67476949	2	2:0|3:2
sgRNA_367	TGCGCGTTGGCGGCCATTAG	chr5	147895627	147895649	-	Nanog	+	147895536	40	1.0	0	2:0|3:0
sgRNA_368	GACAGTAGGACCGCCGCTAA	chr5	147895614	147895636	+	Nanog	+	147895536	48	1.0	0	2:0|3:0
sgRNA_369	AGCGGCGGTCCTACTGTCTA	chr5	147895609	147895631	-	Nanog	+	147895536	56	1.0	0	2:0|3:0
sgRNA_370	TGGCCGCCAACGCGCACAGC	chr5	147895634	147895656	+	Nanog	+	147895536	38	0.77070341	3	2:1|3:2
sgRNA_371	GCTCTGTTGACGGATTTAAT	chr5	20509767	20509789	-	Nanog	-	20509909	30	0.70579108	4	2:0|3:4
sgRNA_372	GAGGCACTCCAGGCGATTAA	chr5	20629355	20629377	+	Nanog	-	20629242	32	0.63505747	3	2:1|3:2
sgRNA_373	CTGAAGGGGGCGTTGGTTAA	chr5	21257541	21257563	-	Nanog	-	21257384	21	0.5720303	5	2:0|3:5
sgRNA_374	TACAGCTCATTAGGTCCTAA	chr5	21718896	21718918	-	Nanog	-	21718843	38	0.83253203	4	2:0|3:4
sgRNA_375	ATTAATGGCCCACTCATACC	chr5	23436848	23436870	-	Nanog	-	23436894	52	0.65696355	3	2:0|3:3
sgRNA_376	AGGTATGAGTGGGCCATTAA	chr5	23436850	23436872	+	Nanog	-	23436894	26	0.67456635	4	2:0|3:4
sgRNA_377	GGTATGAGTGGGCCATTAAT	chr5	23436851	23436873	+	Nanog	-	23436894	46	0.6162424	5	2:0|3:5
sgRNA_378	TACCTAATGGGTTCCCTGCA	chr5	28176690	28176712	+	Nanog	+	28176709	52	0.40804254	5	2:0|3:5
sgRNA_379	AGCAGGCCTTCGGCTCAAGA	chr5	35888601	35888623	-	Nanog	+	35888554	56	0.6686236	4	2:0|3:4
sgRNA_380	CAAGCCCATTAGACGGGAGA	chr5	44144090	44144112	+	Nanog	-	44144020	52	0.4424829	4	2:0|3:4
sgRNA_381	CCGTCTAATGGGCTTGAAGG	chr5	44144083	44144105	-	Nanog	-	44144020	60	0.52816942	3	2:0|3:3
sgRNA_382	CTCCCGTCTAATGGGCTTGA	chr5	44144086	44144108	-	Nanog	-	44144020	43	0.64	2	2:0|3:2
sgRNA_383	AATAGGAGCTTCACTCACTT	chr5	52929951	52929973	-	Nanog	+	52929877	60	0.49035163	5	2:0|3:5
sgRNA_384	AAGAAGATCATCTGGTTAAT	chr5	53505914	53505936	+	Nanog	+	53505950	27	0.60890156	4	2:0|3:4
sgRNA_385	CCCAACTAGTGTAACTAATC	chr5	6517520	6517542	+	Nanog	+	6517505	32	0.5424881	3	2:0|3:3
sgRNA_386	AATTTTCAGGCCCATTAACC	chr5	68004184	68004206	-	Nanog	+	68004142	45	0.67504635	3	2:0|3:3
sgRNA_387	TGTCTAAAACTAGTCCTAAT	chr5	76539963	76539985	+	Nanog	+	76540138	42	0.7855964	2	2:0|3:2
sgRNA_388	GCAACGTACATCCTTCTGTA	chr5	81064358	81064380	-	Nanog	+	81064255	54	0.51992395	3	2:0|3:3
sgRNA_389	GAGACCAATTAAACTAGGGA	chr5	91639519	91639541	-	Nanog	+	91639436	62	0.66412727	4	2:0|3:4
sgRNA_390	GTCAGGCAATTAAGCTAATC	chr5	92112676	92112698	-	Nanog	+	92112697	28	0.77698809	5	2:0|3:5
sgRNA_391	ATTAAGTCGTTAAAAGCTAG	chr5	99552822	99552844	+	Nanog	-	99552778	47	1.0	0	2:0|3:0
sgRNA_392	ACTAGCTTTTAACGACTTAA	chr5	99552820	99552842	-	Nanog	-	99552778	20	0.87549744	2	2:0|3:2
sgRNA_393	TTAAGTCGTTAAAAGCTAGT	chr5	99552823	99552845	+	Nanog	-	99552778	56	0.56464499	4	2:0|3:4
sgRNA_394	TAAGTCGTTAAAAGCTAGTG	chr5	99552824	99552846	+	Nanog	-	99552778	61	0.77241379	1	2:0|3:1
sgRNA_395	GTTTAATGGCCAGACCGTCA	chr6	101272319	101272341	+	Nanog	+	101272411	59	0.45359499	4	2:1|3:3
sgRNA_396	TTTAATGGCCAGACCGTCAC	chr6	101272320	101272342	+	Nanog	+	101272411	49	0.96036585	2	2:0|3:2
sgRNA_397	AGTGTTTTCCCGTGACGGTC	chr6	101272328	101272350	-	Nanog	+	101272411	42	0.73845789	3	2:0|3:3
sgRNA_398	ATTAGAGCTAAGGCGAATGT	chr6	116119874	116119896	-	Nanog	+	116119967	56	0.88888889	4	2:0|3:4
sgRNA_399	ACATTCGCCTTAGCTCTAAT	chr6	116119877	116119899	+	Nanog	+	116119967	49	0.67473354	3	2:0|3:3
sgRNA_400	TTATAAGCAATTAATCACGG	chr6	124804702	124804724	-	Nanog	+	124804621	70	0.46078068	4	2:0|3:4
sgRNA_401	CTAATGGCTGGTTGCGTGTC	chr6	12551358	12551380	-	Nanog	-	12551359	25	0.77173829	5	2:0|3:5
sgRNA_402	AAGGATCGAGAGAATCCTAA	chr6	127769912	127769934	-	Nanog	-	127770027	41	0.5805543	3	2:0|3:3
sgRNA_403	GAGGATGTCACGTACCCATT	chr6	127769897	127769919	+	Nanog	-	127770027	44	0.79295534	2	2:0|3:2
sgRNA_404	AGGATCGAGAGAATCCTAAT	chr6	127769911	127769933	-	Nanog	-	127770027	53	0.44393197	3	2:1|3:2
sgRNA_405	GGTTCCAAGGCCGTTGCATT	chr6	128355316	128355338	-	Nanog	-	128355391	39	0.93295009	4	2:0|3:4
sgRNA_406	GGTCGCGAGTGATGGTTAAT	chr6	128355337	128355359	-	Nanog	-	128355391	33	0.56066033	2	2:1|3:1
sgRNA_407	GTGGGGTTAAGAGCCATTAA	chr6	134976152	134976174	-	Nanog	+	134976220	35	0.72596069	5	2:1|3:4
sgRNA_408	GCTGGCTGCCCTCCCTTAAT	chr6	134981482	134981504	+	Nanog	+	134981439	34	0.61126104	5	2:0|3:5
sgRNA_409	CTGGTGAGCTTTTAATTGCC	chr6	138420004	138420026	+	Nanog	+	138420045	39	0.48352225	3	2:0|3:3
sgRNA_410	GACACCATTGAGTACCCATT	chr6	145234609	145234631	+	Nanog	-	145234590	46	0.47089256	4	2:0|3:4
sgRNA_411	CCATTGAGTACCCATTAGGA	chr6	145234613	145234635	+	Nanog	-	145234590	59	0.50829697	5	2:0|3:5
sgRNA_412	GTACCCATTAGGATGGTTAT	chr6	145234620	145234642	+	Nanog	-	145234590	31	0.49176456	4	2:0|3:4
sgRNA_413	TTCATTAATGGCCCGCATCC	chr6	16607393	16607415	+	Nanog	+	16607424	41	0.78981294	4	2:0|3:4
sgRNA_414	GGCAACCCATTAAATAACAA	chr6	28762599	28762621	-	Nanog	+	28762619	63	0.47969187	5	2:0|3:5
sgRNA_415	ACAGGCCAATTAATGCAGGG	chr6	32056822	32056844	-	Nanog	+	32056800	76	0.4838389	5	2:0|3:5
sgRNA_416	CTGGACAGGCCAATTAATGC	chr6	32056826	32056848	-	Nanog	+	32056800	52	0.60764026	5	2:0|3:5
sgRNA_417	CCCTAATGGGTTTCTTCACA	chr6	33844644	33844666	+	Nanog	+	33844635	45	0.89651844	2	2:0|3:2
sgRNA_418	CTCTACCTGACAGCCAATTA	chr6	34901216	34901238	+	Nanog	-	34901233	20	0.60588061	5	2:0|3:5
sgRNA_419	TCTACCTGACAGCCAATTAA	chr6	34901217	34901239	+	Nanog	-	34901233	40	0.67239262	5	2:0|3:5
sgRNA_420	CCATTAGAATATGACCAGTG	chr6	4677584	4677606	-	Nanog	+	4677669	74	0.50848519	4	2:1|3:3
sgRNA_421	CCTCACTGGTCATATTCTAA	chr6	4677584	4677606	+	Nanog	+	4677669	34	0.84707058	2	2:0|3:2
sgRNA_422	AGCCATTAGCTTTTGTCCGC	chr6	49352978	49353000	+	Nanog	-	49353117	48	0.41510853	4	2:0|3:4
sgRNA_423	AATCGCTTTCATCTCTGGCA	chr6	55586998	55587020	-	Nanog	-	55586995	52	0.65763401	3	2:0|3:3
sgRNA_424	TCTGCATTGATTACCAATTA	chr6	56292381	56292403	+	Nanog	-	56292359	24	0.63995832	5	2:0|3:5
sgRNA_425	TCTTATTCCACTACCTTAAT	chr6	56292394	56292416	-	Nanog	-	56292359	17	0.81279106	2	2:0|3:2
sgRNA_426	ATCGTCATACCAGTAAGTGG	chr6	61524311	61524333	-	Nanog	-	61524251	69	0.75219563	2	2:0|3:2
sgRNA_427	TAATCGTCATACCAGTAAGT	chr6	61524313	61524335	-	Nanog	-	61524251	56	0.55160198	4	2:0|3:4
sgRNA_428	TTAATCGTCATACCAGTAAG	chr6	61524314	61524336	-	Nanog	-	61524251	56	0.87977821	2	2:0|3:2
sgRNA_429	TGCTAATGGCTCTTATGGCC	chr6	71293727	71293749	-	Nanog	-	71293596	43	0.76540967	3	2:0|3:3
sgRNA_430	TCCCTGCTAATGGCCCAACA	chr6	8661769	8661791	-	Nanog	-	8661718	62	0.8013245	1	2:0|3:1
sgRNA_431	CCCTGCTAATGGCCCAACAG	chr6	8661768	8661790	-	Nanog	-	8661718	65	0.69316906	4	2:0|3:4
sgRNA_432	CCCCTGTTGGGCCATTAGCA	chr6	8661768	8661790	+	Nanog	-	8661718	48	0.85585586	1	2:0|3:1
sgRNA_433	GTCCCTGCTAATGGCCCAAC	chr6	8661770	8661792	-	Nanog	-	8661718	35	1.0	0	2:0|3:0
sgRNA_434	TCCCCTGTTGGGCCATTAGC	chr6	8661767	8661789	+	Nanog	-	8661718	33	0.37963915	4	2:0|3:4
sgRNA_435	GTGATGTTCGGCAATTAACA	chr6	88483233	88483255	+	Nanog	-	88483248	52	0.884131	2	2:0|3:2
sgRNA_436	TGATGTTCGGCAATTAACAT	chr6	88483234	88483256	+	Nanog	-	88483248	66	0.90586797	1	2:0|3:1
sgRNA_437	AACGGCCCATTAAGTCTTGC	chr6	89310400	89310422	-	Nanog	+	89310381	37	0.70907821	2	2:0|3:2
sgRNA_438	GCGGGGAGGAAGATCTTAAT	chr6	89381056	89381078	-	Nanog	-	89380979	32	0.91938193	1	2:0|3:1
sgRNA_439	CAGCTCGCAGTTTAATCGCC	chr6	8956788	8956810	-	Nanog	-	8956856	56	0.62041353	2	2:0|3:2
sgRNA_440	GATTAAACTGCGAGCTGAGA	chr6	8956794	8956816	+	Nanog	-	8956856	53	0.6761873	5	2:0|3:5
sgRNA_441	CGATTAAACTGCGAGCTGAG	chr6	8956793	8956815	+	Nanog	-	8956856	64	1.0	0	2:0|3:0
sgRNA_442	AATCTTGGGTGGTCTAATCG	chr6	9002835	9002857	+	Nanog	-	9002899	68	1.0	0	2:0|3:0
sgRNA_443	TTAATGGGCAATTTCCAGCA	chr6	93005888	93005910	-	Nanog	-	93005774	60	0.54634064	4	2:1|3:3
sgRNA_444	GCCATTAGACATCAGTAAGG	chr6	93005789	93005811	+	Nanog	-	93005774	62	0.43289785	4	2:0|3:4
sgRNA_445	TCCGCCTTACTGATGTCTAA	chr6	93005790	93005812	-	Nanog	-	93005774	46	0.39643434	4	2:1|3:3
sgRNA_446	TGTTAATCGCCAGTGTTTTC	chr6	93689347	93689369	+	Nanog	+	93689399	11	0.90430622	1	2:0|3:1
sgRNA_447	TAAGGGGGCAAACTGACTAA	chr6	95013393	95013415	+	Nanog	+	95013328	39	0.76894034	4	2:0|3:4
sgRNA_448	AAGGGGGCAAACTGACTAAT	chr6	95013394	95013416	+	Nanog	+	95013328	45	0.86420427	2	2:0|3:2
sgRNA_449	ATGGGTAGCAATTAACTTTG	chr6	98482628	98482650	-	Nanog	+	98482663	42	0.30250784	5	2:1|3:4
sgRNA_450	TAATCGGCTATGGGTTCCTT	chr7	101627041	101627063	-	Nanog	-	101626972	56	0.60138631	3	2:0|3:3
sgRNA_451	GATAGACGCTAATCGGCTAT	chr7	101627050	101627072	-	Nanog	-	101626972	61	0.88357588	1	2:0|3:1
sgRNA_452	CGGCTATGGGTTCCTTAGGC	chr7	101627037	101627059	-	Nanog	-	101626972	51	0.52304517	4	2:0|3:4
sgRNA_453	AGATAGACGCTAATCGGCTA	chr7	101627051	101627073	-	Nanog	-	101626972	44	1.0	0	2:0|3:0
sgRNA_454	GCTTTGAGATAGACGCTAAT	chr7	101627057	101627079	-	Nanog	-	101626972	43	0.85301837	2	2:0|3:2
sgRNA_455	AATGTCCAATTGGCTCCATA	chr7	115501567	115501589	-	Nanog	+	115501702	49	0.73358174	2	2:0|3:2
sgRNA_456	GTAGGTCACGGTCACAAAGG	chr7	123296301	123296323	+	Nanog	-	123296331	63	0.52613118	5	2:0|3:5
sgRNA_457	TGCATCTGGTACTGGCTTAA	chr7	123296324	123296346	-	Nanog	-	123296331	22	0.56653214	3	2:0|3:3
sgRNA_458	TCACCCCTTAGCTAATGGCC	chr7	129575494	129575516	-	Nanog	-	129575375	53	0.53207497	4	2:0|3:4
sgRNA_459	ACAACCGATTAATTGAATCC	chr7	136846271	136846293	+	Nanog	-	136846283	60	0.94594595	2	2:0|3:2
sgRNA_460	ACGTGCAAGTAATTAATTGC	chr7	137031177	137031199	-	Nanog	-	137031073	40	0.71957767	3	2:0|3:3
sgRNA_461	AGGTTAATCGCCTATAGCAA	chr7	29944501	29944523	+	Nanog	+	29944424	61	0.74686192	1	2:0|3:1
sgRNA_462	CTGGCCATTAATAACACCAC	chr7	30267144	30267166	+	Nanog	-	30267090	56	0.68787411	4	2:0|3:4
sgRNA_463	CGGCACCTTTTCACGTTAAT	chr7	4532517	4532539	-	Nanog	-	4532506	15	1.0	0	2:0|3:0
sgRNA_464	ACCTCTAGTGAATATACTTC	chr7	4870181	4870203	+	Nanog	-	4870355	43	0.70051694	4	2:0|3:4
sgRNA_465	AAGTTAAACACCGATTAGTC	chr7	49915124	49915146	+	Nanog	-	49915154	46	1.0	0	2:0|3:0
sgRNA_466	TGGGCTTAATGGCCCATGAA	chr7	50362169	50362191	-	Nanog	-	50362198	53	0.61956881	4	2:0|3:4
sgRNA_467	CAAGTTACGTTTAATTGCTA	chr7	55405833	55405855	+	Nanog	+	55405957	40	0.48867687	5	2:0|3:5
sgRNA_468	CAAAGGTATCGTAATCTAAT	chr7	63947600	63947622	+	Nanog	+	63947643	41	0.44855959	4	2:0|3:4
sgRNA_469	TAATCTAATCGGTTTCTCCA	chr7	63947611	63947633	+	Nanog	+	63947643	61	0.81109185	2	2:0|3:2
sgRNA_470	TGACTTAATTGGTGTCCATT	chr7	71064641	71064663	-	Nanog	-	71064674	43	0.4725475	4	2:0|3:4
sgRNA_471	GATCTAATCGCTCCCCTAGG	chr7	73618062	73618084	+	Nanog	+	73618069	59	0.69438878	2	2:1|3:1
sgRNA_472	GGAGCGATTAGATCAGGGCG	chr7	73618053	73618075	-	Nanog	+	73618069	63	0.55136172	4	2:0|3:4
sgRNA_473	CCTAGGGGAGCGATTAGATC	chr7	73618059	73618081	-	Nanog	+	73618069	43	1.0	0	2:0|3:0
sgRNA_474	CTAGGGGAGCGATTAGATCA	chr7	73618058	73618080	-	Nanog	+	73618069	62	0.82112466	2	2:0|3:2
sgRNA_475	AAAACCAGCGTACTCATGAG	chr7	81141935	81141957	-	Nanog	+	81141849	64	0.52884412	4	2:0|3:4
sgRNA_476	TGGGCAGACGTTCCTTTTAA	chr8	102698592	102698614	-	Nanog	-	102698458	42	0.68066803	5	2:0|3:5
sgRNA_477	GGCAGACGTTCCTTTTAACG	chr8	102698590	102698612	-	Nanog	-	102698458	64	0.81896552	1	2:0|3:1
sgRNA_478	GGGCAGACGTTCCTTTTAAC	chr8	102698591	102698613	-	Nanog	-	102698458	36	0.63889651	2	2:0|3:2
sgRNA_479	GAACGTCTGCCCATTAAGTA	chr8	102698602	102698624	+	Nanog	-	102698458	48	0.66715773	3	2:0|3:3
sgRNA_480	CTGAGGCGTGGCCATTAATG	chr8	105432254	105432276	-	Nanog	+	105432297	60	0.87837838	1	2:0|3:1
sgRNA_481	TCACAGGCAATTAGGGCTAA	chr8	107519954	107519976	+	Nanog	+	107520040	41	0.82286917	3	2:0|3:3
sgRNA_482	GGCTCGACATGTCTTTATGC	chr8	107519975	107519997	+	Nanog	+	107520040	38	0.86404834	1	2:0|3:1
sgRNA_483	GCTCGACATGTCTTTATGCT	chr8	107519976	107519998	+	Nanog	+	107520040	53	0.99299065	2	2:0|3:2
sgRNA_484	GAACAAAAGCTTCGGCCATT	chr8	109012810	109012832	+	Nanog	-	109012800	26	0.78092734	4	2:0|3:4
sgRNA_485	AACAAAAGCTTCGGCCATTA	chr8	109012811	109012833	+	Nanog	-	109012800	30	0.75291017	4	2:0|3:4
sgRNA_486	TCGGCCATTAGGGGCTCAGC	chr8	109012821	109012843	+	Nanog	-	109012800	38	0.52602893	5	2:0|3:5
sgRNA_487	AATGCGAGCCATTAATCCCT	chr8	115199381	115199403	+	Nanog	-	115199354	52	0.55752659	4	2:0|3:4
sgRNA_488	ACCCATTAACCCCACGAGTA	chr8	22425585	22425607	-	Nanog	+	22425745	52	0.65275459	1	2:0|3:1
sgRNA_489	GCCCTACTCGTGGGGTTAAT	chr8	22425584	22425606	+	Nanog	+	22425745	38	0.45184674	3	2:0|3:3
sgRNA_490	TACCCATTAACCCCACGAGT	chr8	22425586	22425608	-	Nanog	+	22425745	61	0.60707574	5	2:0|3:5
sgRNA_491	CATCTAATCGCTCAACGTCA	chr8	24421076	24421098	-	Nanog	-	24421063	56	0.55644085	3	2:0|3:3
sgRNA_492	CTCAACGTCACGGTCCTTAC	chr8	24421066	24421088	-	Nanog	-	24421063	28	1.0	0	2:0|3:0
sgRNA_493	GACCTCTGGGCAACCCATTA	chr8	4366478	4366500	-	Nanog	+	4366396	38	0.69632077	3	2:0|3:3
sgRNA_494	AGGGCTAAACCACTCCCTTC	chr8	4481803	4481825	-	Nanog	+	4481779	37	0.55253106	5	2:0|3:5
sgRNA_495	GTAGGCGCTCTAATCGCCGG	chr8	47690623	47690645	+	Nanog	+	47690723	61	1.0	0	2:0|3:0
sgRNA_496	TAGGCGCTCTAATCGCCGGT	chr8	47690624	47690646	+	Nanog	+	47690723	63	1.0	0	2:0|3:0
sgRNA_497	TAGGTAGGCGCTCTAATCGC	chr8	47690620	47690642	+	Nanog	+	47690723	61	0.73221757	1	2:0|3:1
sgRNA_498	TGAAAGGGCTTAATCGGCAA	chr8	49195135	49195157	-	Nanog	-	49195016	54	0.78203464	5	2:1|3:4
sgRNA_499	TTGCCGATTAAGCCCTTTCA	chr8	49195138	49195160	+	Nanog	-	49195016	36	0.93333968	2	2:0|3:2
sgRNA_500	TTGCAGCACTAATGGGCTTA	chr8	64629576	64629598	-	Nanog	-	64629588	32	0.66861931	3	2:1|3:2
sgRNA_501	AAGCAATTTGCAGCACTAAT	chr8	64629583	64629605	-	Nanog	-	64629588	42	0.61246172	3	2:0|3:3
sgRNA_502	TGTCACCGCACGCTAATGGG	chr8	65053619	65053641	-	Nanog	-	65053777	64	0.76726343	1	2:0|3:1
sgRNA_503	GACTGCCACCCATTAGCGTG	chr8	65053614	65053636	+	Nanog	-	65053777	67	0.69715995	3	2:0|3:3
sgRNA_504	ACCCATTAGCGTGCGGTGAC	chr8	65053621	65053643	+	Nanog	-	65053777	33	0.8372093	1	2:1|3:0
sgRNA_505	CCCATTAGCGTGCGGTGACA	chr8	65053622	65053644	+	Nanog	-	65053777	47	0.69676993	3	2:0|3:3
sgRNA_506	CCCTGTCACCGCACGCTAAT	chr8	65053622	65053644	-	Nanog	-	65053777	31	0.94570136	1	2:0|3:1
sgRNA_507	ACCCTGTCACCGCACGCTAA	chr8	65053623	65053645	-	Nanog	-	65053777	30	0.56270558	4	2:0|3:4
sgRNA_508	TTATGCTACCCTATGCTAAT	chr8	65064283	65064305	-	Nanog	-	65064231	38	0.53572481	5	2:1|3:4
sgRNA_509	GCGCACCCATTAGCGTCCCC	chr8	72443783	72443805	-	Nanog	+	72443765	55	0.86597938	1	2:0|3:1
sgRNA_510	TTATGACCTGGGGACGCTAA	chr8	72443777	72443799	+	Nanog	+	72443765	34	0.84848485	2	2:0|3:2
sgRNA_511	TATGACCTGGGGACGCTAAT	chr8	72443778	72443800	+	Nanog	+	72443765	42	0.57251908	1	2:0|3:1
sgRNA_512	AATGGGTGCGCTGAAATTAC	chr8	72443795	72443817	+	Nanog	+	72443765	38	0.40827354	4	2:0|3:4
sgRNA_513	GCCTCTCCGGGACTTTTAAT	chr8	73104209	73104231	-	Nanog	-	73104317	28	0.52857643	5	2:1|3:4
sgRNA_514	GCCAATTAAAAGTCCCGGAG	chr8	73104208	73104230	+	Nanog	-	73104317	63	0.65914511	2	2:0|3:2
sgRNA_515	CGTAAGGTGTGGTAATTAAT	chr8	78537903	78537925	+	Nanog	+	78538044	37	0.46369305	3	2:1|3:2
sgRNA_516	TTAATTACCACACCTTACGC	chr8	78537899	78537921	-	Nanog	+	78538044	52	0.58646963	3	2:0|3:3
sgRNA_517	TAATTACCACACCTTACGCT	chr8	78537898	78537920	-	Nanog	+	78538044	55	0.51250104	3	2:1|3:2
sgRNA_518	GCGTAAGGTGTGGTAATTAA	chr8	78537902	78537924	+	Nanog	+	78538044	26	1.0	0	2:0|3:0
sgRNA_519	GCGATTAGCGTCTTCCAGAG	chr8	84257359	84257381	+	Nanog	-	84257442	67	0.79389313	1	2:0|3:1
sgRNA_520	CGCTAATCGCCCGATAACCT	chr8	84257346	84257368	-	Nanog	-	84257442	56	1.0	0	2:0|3:0
sgRNA_521	GCACGCGCTCCTAGGTTATC	chr8	84257337	84257359	+	Nanog	-	84257442	48	1.0	0	2:0|3:0
sgRNA_522	CCGCCGATTAGTAAATTACA	chr8	89003562	89003584	+	Nanog	-	89003679	55	0.83286908	1	2:0|3:1
sgRNA_523	CCTTGTAATTTACTAATCGG	chr8	89003562	89003584	-	Nanog	-	89003679	67	1.0	0	2:0|3:0
sgRNA_524	TACCGTTAATTGCCTTTGTG	chr8	9332253	9332275	-	Nanog	-	9332233	53	0.90073529	1	2:0|3:1
sgRNA_525	ACTTAATGGGTAACCCCCTC	chr8	94035299	94035321	+	Nanog	+	94035286	44	0.64685086	4	2:0|3:4
sgRNA_526	AATAAGATTGCCACGTTAAT	chr9	101034309	101034331	-	Nanog	-	101034388	28	0.64491654	1	2:0|3:1
sgRNA_527	GAGCCTGTGGCCGATTAACG	chr9	101034299	101034321	+	Nanog	-	101034388	63	0.92381488	2	2:0|3:2
sgRNA_528	TTGCCACGTTAATCGGCCAC	chr9	101034302	101034324	-	Nanog	-	101034388	59	0.91549296	1	2:1|3:0
sgRNA_529	CCATTAGAAAATACTTGCTC	chr9	102205745	102205767	+	Nanog	-	102205719	47	0.47301573	5	2:0|3:5
sgRNA_530	CTCCGGTGAGCAGTGGCTAA	chr9	103574715	103574737	+	Nanog	+	103574561	34	0.52162227	5	2:0|3:5
sgRNA_531	CTGAACAAAATAGACGATTA	chr9	103751805	103751827	-	Nanog	+	103751817	25	0.73119455	4	2:1|3:3
sgRNA_532	TGAACAAAATAGACGATTAA	chr9	103751804	103751826	-	Nanog	+	103751817	42	0.61311064	5	2:0|3:5
sgRNA_533	ATATTATTAATGGGCCGTCT	chr9	108711018	108711040	+	Nanog	+	108710965	48	0.47137965	4	2:0|3:4
sgRNA_534	CATTAGCTTGTTAATGAAGG	chr9	110947522	110947544	-	Nanog	+	110947556	59	0.75667117	5	2:0|3:5
sgRNA_535	ATGTGAGACAGGGCGTTAAT	chr9	110953044	110953066	+	Nanog	+	110953108	37	0.71628439	4	2:0|3:4
sgRNA_536	GACAGGGCGTTAATTGGTCT	chr9	110953050	110953072	+	Nanog	+	110953108	56	0.82875264	2	2:0|3:2
sgRNA_537	CGATTAGCATATCAAAGTCT	chr9	123731493	123731515	-	Nanog	+	123731463	53	0.6475054	4	2:0|3:4
sgRNA_538	AGCTTGCTAATCGCTTGTGC	chr9	22210690	22210712	-	Nanog	-	22210812	52	0.68515329	2	2:0|3:2
sgRNA_539	TTGCTAATCGCTTGTGCAGG	chr9	22210687	22210709	-	Nanog	-	22210812	56	0.48289696	4	2:0|3:4
sgRNA_540	TGCTTAATGGCCTCTAAATG	chr9	24160489	24160511	+	Nanog	+	24160496	56	0.71641008	4	2:0|3:4
sgRNA_541	ACTGGCTTAATCGTCTCTAG	chr9	32450238	32450260	+	Nanog	+	32450330	66	0.59520096	3	2:0|3:3
sgRNA_542	CTTAATCGTCTCTAGAGGCC	chr9	32450243	32450265	+	Nanog	+	32450330	46	0.4487852	4	2:0|3:4
sgRNA_543	CGATTAAGCCAGTGCTTTGC	chr9	32450228	32450250	-	Nanog	+	32450330	28	0.41302676	4	2:0|3:4
sgRNA_544	CTCAAGGTAGCCCCAAGATA	chr9	38591180	38591202	-	Nanog	+	38591239	40	0.5397169	4	2:0|3:4
sgRNA_545	GCGAGCCATTAGAGTCACAC	chr9	41899954	41899976	-	Nanog	+	41899974	67	0.64705882	1	2:0|3:1
sgRNA_546	AGGTCCTTCATTCATGCTAA	chr9	42858851	42858873	+	Nanog	+	42858713	35	0.54712065	3	2:0|3:3
sgRNA_547	CCCATTAACTCATGCCCTGC	chr9	43194795	43194817	+	Nanog	-	43194815	32	0.41538005	5	2:1|3:4
sgRNA_548	CCAGCAGGGCATGAGTTAAT	chr9	43194795	43194817	-	Nanog	-	43194815	45	0.46465758	5	2:0|3:5
sgRNA_549	ATGGGCTAATGGGCCTTGCC	chr9	47350882	47350904	+	Nanog	+	47350907	46	0.39581351	3	2:0|3:3
sgRNA_550	GGGTCATTATGATGGGCTAA	chr9	47350871	47350893	+	Nanog	+	47350907	33	0.578548	4	2:0|3:4
sgRNA_551	CCTAGGTTCATTATCTCCAA	chr9	55101335	55101357	-	Nanog	+	55101351	61	0.36612041	5	2:0|3:5
sgRNA_552	CCTTCGTTAATTGCTTAATG	chr9	55225290	55225312	+	Nanog	+	55225328	56	0.57273848	5	2:0|3:5
sgRNA_553	GGCTAATGGCTCGTTATTCT	chr9	58438628	58438650	+	Nanog	+	58438671	28	0.51472726	2	2:0|3:2
sgRNA_554	CTAGCACTGCGGCCATTAGG	chr9	64393938	64393960	+	Nanog	-	64394115	51	0.82292795	4	2:0|3:4
sgRNA_555	GCACTGCGGCCATTAGGTGG	chr9	64393941	64393963	+	Nanog	-	64394115	56	0.4500846	3	2:0|3:3
sgRNA_556	ATCGTTGCTTCCCCGGCAGG	chr9	69907576	69907598	-	Nanog	-	69907620	48	0.41647547	5	2:0|3:5
sgRNA_557	TTAATCGTTGCTTCCCCGGC	chr9	69907579	69907601	-	Nanog	-	69907620	47	0.83884298	2	2:0|3:2
sgRNA_558	ATGGCCCATTTAATTGCTCA	chr9	71348642	71348664	+	Nanog	+	71348622	51	0.70984033	4	2:0|3:4
sgRNA_559	CTATAGTAACCTAATACTGT	chr9	71916302	71916324	-	Nanog	-	71916156	61	0.66936306	4	2:0|3:4
sgRNA_560	CAGCGGAGGAGAGCCATTAG	chr9	77955220	77955242	-	Nanog	+	77955378	53	0.64337216	3	2:0|3:3
sgRNA_561	TAAATACCGTTGGAGCTAAT	chr9	78171505	78171527	+	Nanog	+	78171521	45	0.43423261	4	2:0|3:4
sgRNA_562	ATAAATACCGTTGGAGCTAA	chr9	78171504	78171526	+	Nanog	+	78171521	37	0.42749575	5	2:0|3:5
sgRNA_563	GCGATTAGAGAATGTAGAAT	chr9	97708801	97708823	+	Nanog	-	97708856	39	0.46413861	4	2:0|3:4
sgRNA_564	TGGGCCATTAAGATGCCAAT	chr9	98317996	98318018	+	Nanog	-	98317994	46	0.74941228	4	2:0|3:4
sgRNA_565	TAATTGCCCTCTTATAACAA	chrX	14302351	14302373	+	Nanog	+	14302231	60	0.47994598	5	2:0|3:5
sgRNA_566	GGTAGGTCGTTGAAAACCAT	chrX	145688575	145688597	+	Nanog	+	145688632	46	0.97122302	1	2:0|3:1
sgRNA_567	GCGATTAACCATCTGTCCTT	chrX	166930235	166930257	+	Nanog	-	166930154	49	1.0	0	2:0|3:0
sgRNA_568	GATGGTTAATCGCCTTTCTC	chrX	166930225	166930247	-	Nanog	-	166930154	31	0.64340398	5	2:0|3:5
sgRNA_569	GCCATTAAGCACTGGTATGC	chrX	23117107	23117129	+	Nanog	-	23117226	35	0.53273006	5	2:1|3:4
sgRNA_570	CCATTAAGCACTGGTATGCT	chrX	23117108	23117130	+	Nanog	-	23117226	52	0.80121893	3	2:0|3:3
sgRNA_571	GCGATTAGAGAACCCCCAGA	chrX	60182758	60182780	-	Nanog	+	60182897	61	0.58431721	5	2:0|3:5
sgRNA_572	AAGGCGATTAGCATACATTA	chrX	7870123	7870145	+	Nanog	-	7870192	31	0.82743363	1	2:0|3:1
sgRNA_573	GGCTATTGCCATTAAAGAGC	chrX	98317526	98317548	+	Nanog	+	98317683	46	0.67865097	3	2:0|3:3