FastQCFastQC Report
Fri 24 Feb 2017
sample727_circ_rep3_trim_bowtie.bam

Summary

[OK]Basic Statistics

MeasureValue
Filenamesample727_circ_rep3_trim_bowtie.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047116
Sequences flagged as poor quality0
Sequence length5-76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83930.27544077744332673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGG451.4599832E-435.9578171
TTCCGAT2350.034.4276961
TCCGATC3350.031.8790171
CGGCGTA904.687763E-628.20687563
CGGCGTC1054.9725713E-727.63122463
GAGTCGG650.001178869425.172254
CCGATCT4450.024.7398722
TGCGGCG1354.579666E-621.41012861
GCGTGAA1704.6340527E-520.98269870
GGCGTCT1054.2311958E-420.77583164
CGTGATC3000.020.5074142
ACAGCGG954.7243768E-420.4391821
CGACTGA1157.5031276E-519.7096422
ATCGCGT900.00488755919.7050853
GCGGCGT3850.018.80173162
CGCGGAT1752.3215252E-618.35598856
TGTGCGT5000.018.34487370
ACGTGAT4100.018.1543121
ACGTGAC2057.957715E-817.3649941
ATGGCGG6100.016.51288659
Produced by FastQC (version 0.11.5)