FastQCFastQC Report
Fri 24 Feb 2017
sample727_circ_rep2_trim_bowtie.bam

Summary

[OK]Basic Statistics

MeasureValue
Filenamesample727_circ_rep2_trim_bowtie.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626002
Sequences flagged as poor quality0
Sequence length5-76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23560.3763566250587059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG200.00443611258.80746568
ACAACGC353.8886974E-545.000121
TTCCGAT552.9994408E-740.091011
TCCGATC1400.033.7500881
CCGATCT1600.029.5313242
CGATCTA1054.5082932E-521.0539423
CGATCTT800.004836366519.7380713
ACAAGGA3300.002776728.5909311
Produced by FastQC (version 0.11.5)