FastQCFastQC Report
Fri 24 Feb 2017
sample727_circ_rep1_trim_bowtie.bam

Summary

[OK]Basic Statistics

MeasureValue
Filenamesample727_circ_rep1_trim_bowtie.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832824
Sequences flagged as poor quality0
Sequence length5-76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31050.3728278723956082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG150.0045370258.477722
ACGACGG302.4451028E-548.678661
TAGGCGG300.001432245838.942931
TCCGATC1550.032.03371
ACGTGAT1400.031.2934231
CACCGAG400.00583525529.2388572
GCGGAAG557.40954E-427.2537544
CTCGAGA558.574908E-426.580782
ACGTGAC558.62934E-426.5519961
CCGATCT1950.025.4902862
ACACCGA600.001433380124.339331
TTCCGAT902.5089746E-522.7167071
ACTCGAG650.002283433722.4670731
CGATCTT1559.473297E-920.9012133
TGGCGAG850.006867181518.5765726
CGTGATC1603.2678508E-718.2742862
GTGGCCC1602.8738784E-416.5354965
ACAGGGA4853.6379788E-1212.0442041
ACCCTTG1750.0039641511.68287851
GTGATCA3609.966752E-710.635343
Produced by FastQC (version 0.11.5)