FastQCFastQC Report
Wed 22 Mar 2017
cfdna_022_UMI_03072016_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecfdna_022_UMI_03072016_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51777
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA150.00221288570.014
ATTGACC150.00221288570.03
CCTTTGA200.00691476952.548
TACTGGA200.00691476952.515
GCAGCAT305.8272254E-446.6666648
GTGTTAG350.001244691140.01
GAGTGGG350.001244691140.09
GAACCCA657.856539E-426.92307768
GTGTGTG1450.00465929714.4827599
CGAGCCC3206.350888E-510.937570
TCCGAGC4005.6309575E-59.62568
CCGAGCC3753.1549638E-49.33333469
CTCCGAG4351.4071568E-48.85057567
Produced by FastQC (version 0.11.5)