FastQCFastQC Report
Wed 22 Mar 2017
cfdna_022_03072016_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecfdna_022_03072016_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247579
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1036538.308331576597555No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNN144261.156319559723272No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNN133071.0666258409287108No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNN117010.9378965179760159No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNN87370.7003163727507435No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTNNNNNNNNN49860.3996540499639702No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCNNNNNNNNN49700.39837156604912394No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACNNNNNNNNN43010.3447477073596141No Hit
NNNNNNNNNNNNNNCNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNN40410.3239073437433621No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATNNNNNNNNN39830.31925833955204436No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTANNNNNNNNN38930.3120443675310341No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTNNNNNNNNN38820.31116265983957725No Hit
NNNNNNNNNNNNNNCNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNN36790.2948911451699652No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCNNNNNNNNN36470.2923261773402726No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAANNNNNNNNN34870.2795013381918099No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGNNNNNNNNN33730.2703636402985302No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGNNNNNNNNN31800.254893678075697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTNNNNNNNNN31290.2508057605971245No Hit
NNNNNNNNNNNNNNTNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNN30580.24511473822499416No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNN30560.24495442773563836No Hit
NNNNNNNNNNNNNNCNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNN30070.24102682074642165No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCANNNNNNNNN29000.23245020956588724No Hit
NNNNNNNNNNNNNNANNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNN28420.2278012053745695No Hit
NNNNNNNNNNNNNNTNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNN27860.2233125116726075No Hit
NNNNNNNNNNNNNNANNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNN26470.21217093266238052No Hit
NNNNNNNNNNNNNNGNNNNNNNNNNNNCNNNNNNNNNNNNNNNNNNNNNN25830.20704099700299539No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNNNNNNN24330.19501771030131157No Hit
NNNNNNNNNNNNNNGNNNNNNNNNNNNTNNNNNNNNNNNNNNNNNNNNNN23230.18620063338674345No Hit
NNNNNNNNNNNNNNTNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNN22810.18283411311027198No Hit
NNNNNNNNNNNNNNCNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNN22280.1785858851423437No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGNNNNNNNNN21830.17497889913183853No Hit
NNNNNNNNNNNNNNANNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNN21810.17481858864248276No Hit
NNNNNNNNNNNNNNGNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNN18730.150130773281692No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNTNNN18490.14820704740942256No Hit
NNNNNNNNNNNNNNTNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNN17370.13922966000549866No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNTNNN16320.13081335931431998No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNCNNN15660.1255231131655791No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNN15610.12512233694218963No Hit
NNNNNNNNNNNNNNANNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNN15530.1244810949847665No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNCNNN15270.1223970586231413No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNCNNNNNNNNNN13610.1090912880066112No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNCNNNNNNNNNN13380.1072477173790197No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNTNNN13180.10564461248546184No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNTNNNNNNNNNNNTNNNNNNNNNN13150.10540414675142817No Hit
NNNNNNNNNNNNNNGNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNN13100.1050033705280387No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNANNN13000.10420181808125978No Hit
NNNTNNNNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNTNNNNNNNNNN12720.1019574712302788No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCNNNANNN12520.10035436633672097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG409.3164406E-426.17949356
AGTGTCC800.001898855323.14771839
GAGTGTC1254.9621733E-520.74035638
AGAGTGT1156.7873317E-419.32331337
AGATCTC750.001779920116.75487555
AAGAGTG1400.002537049415.87272136
AAAGAGT1756.5036135E-414.81453935
TGTAGAT1151.2325756E-414.56945752
AGTGTAG900.005993651713.96239750
TAGGGAA1900.001211802513.6449730
GTAGGGA1950.001474118113.29509829
GGGAAAG2100.00257378112.34544932
GTAGATC1200.00280846312.21709753
GGAAAGA2150.003070297212.05834633
AGGGAAA2200.003646789111.78429231
GAAAGAG2350.00596548211.032104534
CGGAAGA3308.8412125E-57.44288915
GAAGAGC3256.037795E-46.97605717
TCGGAAG3602.5826425E-46.82264914
GGAAGAG3703.6099553E-46.638252316
Produced by FastQC (version 0.11.5)