FastQCFastQC Report
Wed 22 Mar 2017
cfdna_022_03072016_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecfdna_022_03072016_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247579
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTGCAATCTCGTAT12840.1029193341664135TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA500.00721060327.99999840
GCTCGTA1601.1865777E-619.687555
AGCGGCC1200.001341155717.554
TCGGAAA2552.1766027E-816.47058970
AGCTCGT2009.964915E-615.74999954
AAGCTCG2253.0334939E-514.053
CCGGCTA2556.460623E-613.72549138
CGGCTAA2303.7306727E-513.69565339
TGCCCGG2901.6659578E-613.27586335
GCCCGGC5203.6379788E-1212.78846236
TCGGCTC2701.6722346E-411.6666669
TATTCCG2100.004007412611.66666657
CCATAGT3651.7236234E-611.5068491
CCCGAGT3054.0652572E-511.475411
CGAGTAG2700.002184568410.370373
GCTTGAA17750.010.25352270
CGCCTGG3451.421042E-410.14492835
TCGTATT3156.956023E-410.054
CGTCTTC29100.09.86254364
ACAGGCG6755.4205884E-109.85185118
Produced by FastQC (version 0.11.5)