FastQCFastQC Report
Wed 22 Mar 2017
cfdna_022_03072016_R1.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecfdna_022_03072016_R1.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243816
Sequences flagged as poor quality0
Sequence length5-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG700.001127937225.36202247
AGACGGT800.002905658421.55461526
ACCGCGC1900.00203077412.77431231
CACCGCG2000.003017191512.1177530
CAGGCGC2953.5934747E-511.61480419
CCGGCTA2150.00474331211.40603438
CCCGGCT3501.3676137E-510.99395637
ACAGGCG6757.6397555E-1110.14334218
CGCCCGG2800.00303596639.96700335
CCGGGTT2950.0038210249.69371552
CCAAGTA8800.09.4217542
CCTAATA5851.3592762E-79.0662741
CGCCACC3450.00181431789.00462227
CCAAGAA9005.456968E-128.8396171
GCCCGGC4754.0576542E-58.82376236
GCCACCG4704.0778486E-58.82005528
GCCCGCC3400.00229538848.774621
GGGACTA7202.7439455E-88.50965612
CCCAAAG18400.08.4672971
GCGTGAG5301.9206114E-58.42988522
Produced by FastQC (version 0.11.5)