FastQCFastQC Report
Wed 22 Mar 2017
Undetermined_S0_L001_R1_001_ACAGTGAT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001_ACAGTGAT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10318.327402135231317TruSeq Adapter, Index 5 (100% over 50bp)
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCTAGAAATAGCAAGTT498.718861209964412No Hit
GCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT447.829181494661921No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT274.804270462633451No Hit
TGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT213.7366548042704624No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC101.7793594306049825TruSeq Adapter, Index 5 (98% over 50bp)
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT91.601423487544484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC81.4234875444839856TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTATGC71.2455516014234875TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC61.0676156583629894TruSeq Adapter, Index 5 (98% over 50bp)
AGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.8896797153024912No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGTGATCTCGTATGC50.8896797153024912TruSeq Adapter, Index 5 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC40.7117437722419928TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCCCGTATGC40.7117437722419928TruSeq Adapter, Index 5 (98% over 50bp)
TGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.7117437722419928No Hit
GCAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.5338078291814947No Hit
AAAACCCTCCTCAAACAGCTCAAACCGGTGAGATCGGAAGAGCACACGTC20.3558718861209964No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCACAGTGATCTCGTATGC20.3558718861209964TruSeq Adapter, Index 5 (98% over 50bp)
AGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.3558718861209964No Hit
GATCGGGAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC20.3558718861209964TruSeq Adapter, Index 5 (98% over 50bp)
CAGTGGTATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.3558718861209964No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.3558718861209964No Hit
TTAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.3558718861209964No Hit
CCGCTCCCTGTCTGACGCATACACTAAAGATAACATTACAGTTTGAGGAG10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGATTTCGTTTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
GTCCTGGATTTCTCTTTATTTCCATCTTTACCCGTTGAATTGCTCCCAAG10.1779359430604982No Hit
CCTCAGCTTCCCGAGTAGCTGGGATTACAGGTACGCACCACCATGCCAGA10.1779359430604982No Hit
GATCGGAAGATCACACGTATGAACTCCAGTCACCCAGTGAACTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGTGATCTCGTCTGC10.1779359430604982TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTTTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 46bp)
GACTTCAAAGGTCCTAGTCCAGCCCCCCATGAATGAACCCACGTGGCAGA10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGGAGGGTTTTAGAGCTAGAAATAGCAAGT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCTGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTATAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
GATCGGAAGAGCACTCGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGA10.1779359430604982TruSeq Adapter, Index 5 (97% over 49bp)
CGGGCCGTGCAAGGTAGGCTCACCAAGTCCTCGTGCGTTACACAGGTACA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTGTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGTGATCTCGTATTC10.1779359430604982TruSeq Adapter, Index 5 (97% over 48bp)
TCCATCGGGCACCTTCGGCTCACCAAGTCCTAGTGCGTTACCCAACAACC10.1779359430604982No Hit
GACCGGAAAGAGCACCCGTCTGAACTCCAGTCACACAGTGAACTCGTATG10.1779359430604982TruSeq Adapter, Index 5 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAAGTGAAATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
CCGCTCCCTGTCTGACGCATACACTAAAGATAACATTCTTGTTTCCACAT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GCCGCTCCCTGTCTGACGCATACACTAAAGATAACAGTATTGTTTCCACA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTTGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 43bp)
GTGTACAACCATGGACAAGCAGATGCAGTCCCTATTCTCATACATCTCAT10.1779359430604982No Hit
CACCGGTTTGAACTGTTTGAGGAGTGTTTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCACAGTGATCTCGTATGA10.1779359430604982TruSeq Adapter, Index 5 (97% over 49bp)
GTCAACTGGCTTTGTTTCTCTTCCCCAAGAGTCACTGTAGGCCAGGAACA10.1779359430604982No Hit
GCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
CCCTCAGCCTCCTGGGTAGCTGGGAGCACAGGTGCATGCAACCACACCTG10.1779359430604982No Hit
AACAGCTTCTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCA10.1779359430604982No Hit
GGAATCAACGCCGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
TCCATGTCTTCTGGGAACTTGATGGACACGTCTGAACTCCAGTCACCTCT10.1779359430604982TruSeq Adapter, Index 1 (95% over 24bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
TGCAGTGGTATCAACGCAGAGCCGACTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
ACTTGTGCTTTGCAAATACTGCGTTTTTTTACAACTTAAAAGTTTGTTAC10.1779359430604982No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCGAACGGAAAAGCCCACGT10.1779359430604982Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGTGAGCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
TTACCGTCTTTCCAGAAATTGTTCCAAGTATCGGCAACAGCTTTATCAAT10.1779359430604982No Hit
AAAGTTGAGCAACTCCTGCTCAACTCTGGACTGGGAATTATTACTGAAAA10.1779359430604982No Hit
TATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA10.1779359430604982No Hit
GATCGTAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
TGCAGTGGTATCAACGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
GATGGTTCACTTAGATTAAGATGAGTCAGGGGAGAGTAACACGTCTGAAC10.1779359430604982No Hit
ACTGTACTAACAACTGCCTTTGCCAGATATTTACAACACACGTCTGAACT10.1779359430604982No Hit
AAATGGTCGTACGGTGAGCTCACATTAGAGTGGCCGGGTTCTAGAGTAAT10.1779359430604982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAACACAACGTTCCAGCCGACAATC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTCACAGTGATCTCGTACGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 47bp)
CCGTCTCTACTAAAAATACAAAAATTAGCTAGGTGCCGTGGCTCAAGCCT10.1779359430604982No Hit
TGTAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
CCCACATACACACACGCTTACCCACACATGTACACTCACAATACAGTCAC10.1779359430604982No Hit
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCCAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 23 (97% over 36bp)
CAGTGATCTCGTATGCCGTCTTCTGCTTGGAACACAAGACTGAACTCCAA10.1779359430604982Illumina PCR Primer Index 5 (100% over 29bp)
GAGTAACTACCTTTTAGGTGTTAAGTTTGAGAAGCAATTTTATTTTATTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTGATATCGTATTC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
ACTTGAGAAAGGTCTTAGCATGAAGCACATCTCAGTGGGAGGAAGTGGCC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTTAACTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GTGGAAGTGATATACATAATTTCCAGGCCTGGCCATAAAACACCATGGAT10.1779359430604982No Hit
ATTTTAACTTGCTATTTCTAGCACTAAAACCCTCCTAAAACAGCTCAAAC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCCGTCCCACAGTGTTCTCGTTTGC10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGTGATTCCATTCCATTCCATTCCATTGAATTCCATTCAACTCTGGTTG10.1779359430604982No Hit
ATATTGGATGGATGGATGGATGGATGGATGGATGGATGGATGGATGGGTG10.1779359430604982No Hit
CAACGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCTAGAAATCGCCAGTT10.1779359430604982No Hit
TGGGATAGCGTTTCTTTTCTTTCAATCCTTGGCCTTTTTGTATTTTCTCA10.1779359430604982No Hit
TACCAGGGCAAGAATCGGCTCACCAAGTCCTAGTGCGTTACCCACCTAAC10.1779359430604982No Hit
GACCTTGGTAAAGGTCGCTCCCTGTCTGACGCCTACACTAAAGATAACAT10.1779359430604982No Hit
GAGCGAGACTGTCTCAAAAAAAAAAAAAATCCCTAAATTTTGAGTACTTT10.1779359430604982No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC10.1779359430604982No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACCGTGATCTCGTATGCCGT10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GCTGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
ATCCCCGAGTCCTTCAATGCTATCATTTCCTTTGATATTGGATCATATGC10.1779359430604982No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
TCCTTGCGTCACTCTGGGCTCACCAAGTCCTAGCGTTACCCATTACCTAG10.1779359430604982No Hit
TGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTGTTTTTTTTTTTT10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCTCGCAATAGCAAGTT10.1779359430604982No Hit
TATAAATAGCTCATACACATCAATAAGAAAATATTAAGACGCCAAAGGTA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTAAGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 47bp)
CACCGGTTTGAGCTGTTTAAGGAGGGTTTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
ATAACATCATGGTAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTATG10.1779359430604982No Hit
TTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCT10.1779359430604982No Hit
TCCACTAAGCAGCGCTCCCTGTCTGACGCATACACTAAAGATAACATTAT10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCTAGAAATAGTAAGTT10.1779359430604982No Hit
CACCGGTTTGAGCTGTATGAGGAGGGATTTAGAGATAGAACTAGCAAGTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAATGATCACGTTTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGACACACAGTGACCACGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACAGTGACCTCGTATG10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
TGGCTATGGAAGGTTGGGCTCACCAAGTCCTAGTGCGTTACCCAAGGAAC10.1779359430604982No Hit
GTGCTAGGATTACAGGCATGAGCCACCATGCCCAGTCAAAGTCAACCTTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTTAACTCCAGTCACACAGTGATCACGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGA10.1779359430604982TruSeq Adapter, Index 5 (97% over 49bp)
CCACAATAAAAAGGAAGAAATTACTGAATCACTTAACAACATGGATGAAT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCAAGTCACACAGTGATCTCGTAGGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 47bp)
GCTCCCTGAGGGCAGGGAGTTTTGTCTGATTTGCTCTCTGCTGATTTCAT10.1779359430604982No Hit
GCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTCCTGAATCTC10.1779359430604982No Hit
GATCGGAAGAGTACACGTCTGAACTCCAGTCACACAGTGAGATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGTGATTTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACCCGTCTGAACTCCAGTCACACACTGAACCCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 36bp)
GATTGGAAGAGCACACGTCTGAACTCTAGTCACACCGTGATATCGGATGC10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCTATAAATAGCAAGTT10.1779359430604982No Hit
GTCCTTCACATGGACTCTGTGATTATTTTAGAAAAGACTAACGCAAAGAA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATCC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAAACACAGAGA10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTATAGCTAGAAATAGCACGTT10.1779359430604982No Hit
TGGTATCAACGCCGAGTCGACTTTTTTTTTTTTGTTTTTTTTTTTTTTTT10.1779359430604982No Hit
GTGGTATCAACGCAGAGTCTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
TACATGCCTAGAAAATGAGGGGTGGAAATTATATATGCTCATGAAATAGT10.1779359430604982No Hit
GGTGGATCCTTTTATGCGAGCCCTCCTCAGTCCTGCAGCCAGCAGGGACC10.1779359430604982No Hit
TTTTACTTCTCTTCCCCTTAACTTATATAAATGCAAATTTTCCTATACTG10.1779359430604982No Hit
GGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATTC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATAGCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
CAGTGGTATCAACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
TGGAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
AAAACCCTCCTCACACAGCTTAAACAGGTGAGATCGGAAGCGCACACGTA10.1779359430604982No Hit
AGTTCCTGGAACTACCGGCTCACCAAGTCCTAGTGCGTTACCCAGCATGT10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGCCGAGGCAGGAGAATGGCATGAACC10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGGATTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
GAAAATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCTCTAATCGG10.1779359430604982No Hit
TGCAGTGGTATCAACGCAGAGGCGACTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
CCAAGGACCCACAGTGAGGATGGGGATGTCTCTGCCCTGACTTTCAGGTC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGATATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTCTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 46bp)
AACAGCAGCACCAGCTCATCCGTGGGTCTATATCTCCACGACCCTCAAGC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACCTCTATCC10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCACTCGTATT10.1779359430604982TruSeq Adapter, Index 5 (97% over 39bp)
CATCGGTTTGAGCTGTTAGAGGAGGGTTTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCGTTTTC10.1779359430604982TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATAC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
CCCTTAGAATGTCCCTGTATGGCAGATGCACCTGCATGTGTGTTTTGAGC10.1779359430604982No Hit
GCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTGCA10.1779359430604982No Hit
CTAAAACCCTCCTCAAACAGCTCAAACCGGTGAGATCGGAAGAGCACACG10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCACGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACACGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACCCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGACATAGTATTC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTGTCCC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTAGGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 47bp)
GATCGGAAGACCACAAGTCTGAACTCCAGTCACACAGTTCGCTCGTATAC10.1779359430604982TruSeq Adapter, Index 5 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACTTCGTCTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGGTCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
CACTTAGACCCTAAATTTATTTTCCCCCACCTGCCCCACATGACCAAGTA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCACTCGTTATC10.1779359430604982TruSeq Adapter, Index 5 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAATTCTAGTCACACAGTGAACTCGTATGC10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGCAGTGGTATCAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCCGTCACACCGTGAACTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACATTGAAATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
TATTTGTCTTCGTGTGCCTGGCTTATTTCATTTAACACACTTCTGAACTC10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCCAGAAATAGCACGTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAATGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTTCTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACAGTGATCTCGTATG10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCATGCTGCTATAAAGACACACGCTGAACTCCAGTCACCTCTGCAAACT10.1779359430604982No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACCCGTATG10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
TTTTTTTTTTTGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGAC10.1779359430604982No Hit
CACCGGTTTGAGCTGTTGGAGGAGGGTTTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
ATTATATACATTTATAGAAAAATTTCCACTTGCACTTCTGGGTTTAAAAG10.1779359430604982No Hit
ATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAG10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTTACACAGTTATCTCGTTTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATACAGTGATATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 41bp)
GTGGACTGAATGTGTCCCCTGCAAAATTCGTAGTTGAAACCCTCATCCTC10.1779359430604982No Hit
GATCGGAAGCGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 37bp)
GCAGTGGTATCTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
GGGCTTGCCTTAAGCCAGGCACTTCTGACTGCAGTTCTAAGCCAGGAATT10.1779359430604982No Hit
GCTTATGTAACCACACACCACCTGTACCCAATAATTTATGGAATAAATAA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACCCCCGTCACCAAGTGATCGCGTATGC10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT10.1779359430604982TruSeq Adapter, Index 5 (100% over 50bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGTGATCGCATACGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTAAGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 47bp)
GTTGAGACTTTTAGATGTATCTCTGAGCTACATGAAAGCTAACTTGGTGA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTCTGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATTC10.1779359430604982TruSeq Adapter, Index 5 (97% over 48bp)
TGTATATACACATACATATCCCACTTCACTAAAATAAATGACTAAAATGG10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAAATCGTATGT10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
ATGATGTTGAACTCACAGCTCAACATTATAGGTCCTAACATCACCAATTA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATTTCGTAGGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 47bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAAATCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACACAGTGCTCTCGTATTC10.1779359430604982TruSeq Adapter, Index 5 (97% over 39bp)
GCCCATAGCCACTGTAGGCACTGAAGTTTGTTGAAAAAAGAAGAAAATGT10.1779359430604982No Hit
GCTAACAGGTCCCCTGCCCCCACCCCACAGGACACACCACAGGAACTGCA10.1779359430604982No Hit
CAAATAAAACAACACAGGCTCACCAAGTCCTAGGCGTTACCCACAACGAC10.1779359430604982No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACC10.1779359430604982No Hit
CGGAAGAGCACACGTCCGAACTCCAGTCACACAGTGATCTCGTATGCCGT10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTAGCATGG10.1779359430604982TruSeq Adapter, Index 5 (97% over 43bp)
CTAAAACCCTCCTCAAACAGCTCAAACCGGTGAGATCGGAAGAGCACCCG10.1779359430604982No Hit
CCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.1779359430604982No Hit
TGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTCC10.1779359430604982No Hit
CCCACATCCTGCTGATTGGTCCATTTTACAGAGAGCCTGAGTGGTCTGTT10.1779359430604982No Hit
AGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTGCACCGAC10.1779359430604982No Hit
CATACAGCCTGCTACCAAGTGCTGATATAAGGTGTGAAGGAAGGTTAGCT10.1779359430604982No Hit
GCTACGCACCCACTTACTTTCGCCCGGAGGGGCAGCCACCCCCCATGTCT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGGGACATCGTCTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTCTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 46bp)
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCATGAAATAGCAAGTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGACCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
TTAAGAGAGAACCGGTTGCTCACCAAGTCCTAGTGCGTTACCCAATTGCC10.1779359430604982No Hit
GTATCCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTACACC10.1779359430604982No Hit
GTTCGGAAGAGCACACGTCTGAACTCCCGTCACACCGTGATCTCGTATGC10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
CACCGGTTTGAGCTGATTGAGGAGGGTTTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GCAGTGGTATCAACGCCGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
CCAAAGTTAATCTCATTTAGGACTTTATCCTTCTATGCCCCACTGCCCTT10.1779359430604982No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTGC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGATCGTATAC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
CACCGGTTTGAGCGTTTGAGGAGGGTTTTAGAGCTAGAAATAGCAAGTTA10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGGTTTTAGAGCTAGAAATAGCAAGGT10.1779359430604982No Hit
AAGCCTTACATATTTCCTGACATGTTGAAACTACTAGGAAAGCTGTCTCT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTTGATCTCCAGTCACACAGTGAACTCGTATG10.1779359430604982TruSeq Adapter, Index 5 (96% over 26bp)
GATCGGAAGAGCTCACGTCTGAACTCCAGTCACACAGTGAATTCGTAAGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GTTTGGTGGTGGATGCCTGTAATCCCAGATAATTGGGAAGCTGAGGCGGA10.1779359430604982No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGCC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)
GAAGAAAATATAGGAAACACCATTCTGAACTCCAGTCACCTCTGCAATCT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGAACTCCTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGGCACACCGTGACCTAGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 35bp)
GGCGTTGAGCAATTGCCGCTCACCAAGTCCTAGTGCGTTACCCAAGCTGC10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCACAGTGAACTAGTCTGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGAACTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
AGCTCTAAAACCCTCCTCAAACAGCTCAAACCGGTGAGATCGGAAGAGCA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTTATCTCGTTCTC10.1779359430604982TruSeq Adapter, Index 5 (97% over 46bp)
GTATGAGATGTTACCTCACACTCTATAAATATTAGTTAATTTTGAGTCCT10.1779359430604982No Hit
TGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAAA10.1779359430604982No Hit
AACAAAATGTACTCTGGGCTCACCAAGTCCTAGTACATTACTTTCCCTGA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCACAGTTATCTCGTAAAC10.1779359430604982TruSeq Adapter, Index 5 (97% over 38bp)
ACCGCTCCCTGTCTGACGCATACACTAAAGATAACATTATCAAAAAAGGG10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCCGTCACACAGTGAGCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (97% over 40bp)
GCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTCTTTTT10.1779359430604982No Hit
ATGTTGGTGACATCTGCCAGAAATGCATTTCACCTAGCATGCATGCTTTG10.1779359430604982No Hit
TGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACACAGTGATCTCGTATG10.1779359430604982Illumina PCR Primer Index 5 (96% over 29bp)
GAGCGGAAGAGCACACGTCTGAACTCCCGTCACCCAGTGATCTCGTATGC10.1779359430604982Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CACCGGTTTGAGCTGTATGAGGAGGGTTTTAGAGCTAGAAATAGCAAGTT10.1779359430604982No Hit
CACCGGTTTGAGCTGTTTGAGGAGGATGACGATGTCGATACCAAGAAGCA10.1779359430604982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAACAGTGATCTCGTATGC10.1779359430604982TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGA202.790017E-570.034
ACCGGTT150.001218903870.02
TTTGAGG150.001218903870.016
TTTGAGC150.001218903870.07
TAGAAAT150.001218903870.036
ACACGTC150.001218903870.013
GTATGCC202.790017E-570.045
GGGTTTT150.001218903870.024
ATCTCGT150.001218903870.040
CAGTCAC202.790017E-570.027
GTCACAC202.790017E-570.029
CACACGT150.001218903870.012
GCAAGTT150.001218903870.044
CACACAG202.790017E-570.031
GAGGAGG150.001218903870.019
ACGTCTG150.001218903870.015
ATAAGGC150.001218903870.054
GAAATAG150.001218903870.038
TGCCGTC150.001218903870.048
GCTAGAA150.001218903870.034
Produced by FastQC (version 0.11.5)