FastQCFastQC Report
Wed 8 Feb 2017
IH03.trim.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameIH03.trim.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96769121
Sequences flagged as poor quality0
Sequence length1-39
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
*15922691.6454308807868574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT28150.016.2287912
AGCGTCG32700.016.17859611
CGTCGTG29400.015.70983113
TCCGATC61200.015.3107041
GATCGGA77600.014.4227812
AGATCGG83000.014.2800231
CCGATCT67800.013.6229222
GAGCGTC46350.012.54638610
TCGGAAG96600.012.5177374
CGATCTA38050.012.09172253
ATCGGAA97650.012.014643
CGGAAGA107300.011.8955765
AGAGCGT63950.011.1213549
GTCGTGT36200.011.08093314
GAACGGG38550.010.357783
GCGTCGA16450.09.98973312
AAGAGCG89400.09.4798158
CGTCGAG18450.09.26508713
TCGTGTA40900.09.00697815
TTCCGAT43500.08.0493741
Produced by FastQC (version 0.11.5)