FastQCFastQC Report
Tue 31 Jan 2017
IH03.filt.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameIH03.filt.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76561047
Sequences flagged as poor quality0
Sequence length14-39
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC31850.015.9710391
CCGATCT38050.013.28014852
CGATCTA24350.010.846373
CGAAGTG37900.010.4774861
TTCCGAT24950.07.2344091
GGATACT110600.06.9690926
AGGATAC132400.06.0325615
GCACTTG142300.05.508109613
CGATCTT34350.05.34870623
CTTGGGT173000.05.30942444
GCCCAAC82600.05.26010111
GATACTG152350.05.2417267
GAGGATA159650.05.11641744
ACTTGTC143300.05.099099615
ATACTGC155150.05.041028
Produced by FastQC (version 0.11.5)