FastQCFastQC Report
Sun 29 Jan 2017
IA01.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameIA01.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102170165
Sequences flagged as poor quality0
Sequence length1-39
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGGTA1631160.15965130329387253TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG1867900.031.86471416
TCGTGTA1966450.031.60767215
GTCGTGT2033750.031.41681714
CGTCGTG2081800.031.17772913
GCGTCGT2095100.030.97577512
AGCGTCG2110300.030.80089211
GTAGGGA1930450.030.6701619
GAGCGTC2128250.030.53904310
TGTAGGG1959000.030.45766818
AGAGCGT2165200.030.121629
GTGTAGG1994300.029.87410217
ACCCGGT2030250.029.73858533
AAGAGCG2222150.029.584098
TAGGGAA2024000.029.35279520
TCACCCG2067400.029.34469631
CCCGGTA253750.029.1679833
AGATCGG4422850.029.1549321
CACCCGG2105200.029.04926932
CGTCTGA2241900.028.49539617
AGAGTGT2127600.028.4714827
Produced by FastQC (version 0.11.5)