FastQCFastQC Report
Mon 6 Mar 2017
BH01.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameBH01.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2957740797
Sequences flagged as poor quality0
Sequence length30-101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATCGCTCATCTCG47137940.15937143663099698Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11405550.078.352061
ATCGGAA12493150.071.699422
TCGGAAG12921400.068.597843
GAGCGGT11537150.066.7631459
CGGAAGA14049250.062.7927974
AGAGCGG13135300.062.4549678
AAGAGCG14765300.058.9312327
CGTATCG9588050.039.4995734-35
GTATCGC10187400.036.30078536-37
GCGGTTC10809850.036.04668410-11
TATGCCG10591550.035.3921950-51
GACCGTA10674800.035.1825232-33
TATCGCT11021800.033.99550236-37
CGTATGC11065450.033.96148348-49
CTCGTAT10853300.033.78195646-47
ATGCCGA11862400.033.22763424-25
AGCGGTT11686400.033.1513210-11
ATCGCTC11535600.032.96180738-39
ACCGTAT11773900.032.62212432-33
TGCCGTC11415500.032.3025352-53
Produced by FastQC (version 0.11.5)