FastQCFastQC Report
Sun 17 Jul 2022
rep2.r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep2.r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24040621
Sequences flagged as poor quality0
Sequence length41
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13045325.426365650038741No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10376524.316244576211239No Hit
GTTAGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1369340.5695942712960701Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCT761590.31679298134603096No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTGG548680.22823037724358286No Hit
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTA498140.20720762579302757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAT86650.032.88478533
TAGTATC87000.032.73294434
ATACTTA119850.032.2674751
GATCACG70100.031.58444228
TGTAGAT159250.031.247935
GTTACTA60900.031.21302835
TGTAGTA94700.031.19814932
CGTATAA37200.031.19021223
ATACCAT76550.031.1638721
TACTTAC116650.031.0711332
TAAGATC101750.031.02859922
ATCACGA71550.031.01849429
ACTATAC77050.030.904434
CTATACT76650.030.8833035
AAGTGTA96750.030.71834429
TCACGAA77250.030.518930
ATCAAGT98300.030.44552226
GATCAAG100550.030.19893525
TATACTT79350.029.8995766
GACTATA83600.029.7556863
Produced by FastQC (version 0.11.2)