FastQCFastQC Report
Mon 18 Jul 2022
rep2.r2.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep2.r2.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23586622
Sequences flagged as poor quality0
Sequence length14-41
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12770265.414196233780318No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10376464.399298890701687No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCT761590.3228906623424075No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTG548680.23262339134446636No Hit
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTA498140.2111959906764097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTA62300.085.95116435
AGTATCT105450.078.4021835
CGTGTAT13050.065.005935
TCGCCTT75950.053.84624535
TAGTATC91600.048.88368634
GTAGTAT91950.045.33579333
GTTTATC23150.043.211235
GTGACTA16700.041.98911735
TGTAGTA99750.041.55932
TGTTACT74950.038.75228534
GTGTTAC70550.038.4796433
TCACGAA77700.038.2861430
ATCACGA71250.038.10146729
TGTGTTA69150.037.76989732
GATCACG70100.037.69513728
AAGTGTA100600.037.625729
ACGAAGG85100.036.9283432
GTGTAGT110200.036.6974131
TAGTGTG70400.036.14949829
CAAGTGT102700.035.93586328