FastQCFastQC Report
Sun 17 Jul 2022
rep2.r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep2.r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24040621
Sequences flagged as poor quality0
Sequence length48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCGT1228860.511159840671337No Hit
GCCTAACAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGG244670.10177357731316508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA396000.039.9706274
TAACGCT1427400.039.9290774
CTAACGT2009750.039.888273
CTAACGC6310850.039.834313
TAACGCC1680950.039.8268134
CTAACGG4719500.039.8095663
TAACGGC2336400.039.8076934
TAACGTT416350.039.794664
TAACGCA1668900.039.7930564
TAACGTG488100.039.651124
TAACGGG1084700.039.6342354
TAACGGA828050.039.631544
TAACGAG481700.039.6192054
TAACGTC715650.039.608024
TAACGCG1550000.039.533264
TAACGGT486750.039.4934
CTAACCG1076050.039.277153
AACGCAT175400.039.2101175
CTAACCT1823400.039.198333
CCTAACG14982450.039.1809582
Produced by FastQC (version 0.11.2)