FastQCFastQC Report
Mon 18 Jul 2022
rep2.r1.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep2.r1.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23586622
Sequences flagged as poor quality0
Sequence length14-48
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGG244670.10373253109326125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAA10850.0372.833636
ATAATAC11700.0339.3446739
GTATAAT13650.0293.610937
ATACTAA13850.0283.9622242
TATAATA14500.0273.8160438
AATACTA14750.0266.635741
TAATACT14900.0266.4652740
TATGGTT10350.0224.3739236
TGCTATG18900.0217.997738
GTGCTAT21050.0197.5112937
ATGGCTA22850.0180.3131942
TATGGCT23400.0176.0750641
GCTATGG24000.0171.6731939
TTTCTGT91650.0167.9694738
CTATGGC25650.0160.6298840
ATGCCCA35900.0159.6312940
CTTTCTG93750.0159.412637
TCTTTCT97000.0154.8437336
TTCCGTT15300.0151.7823542
CTTCTGT68900.0150.5849537
Produced by FastQC (version 0.11.2)