FastQCFastQC Report
Sun 17 Jul 2022
rep1.r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep1.r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656857
Sequences flagged as poor quality0
Sequence length41
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT53308711.447356017159214Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2813106.040769557665181No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1686783.6221425738432593No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA679811.4598043272533385No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553481.18852693995113No Hit
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTA375450.8062304683180094No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTGG313870.6739953578132204No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGG168150.36108044545924434No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT155390.3336799906031042No Hit
GACTTGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA142390.3057641666901088Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGGGGGAGAGGCTGTCGCTGCGCTTCTGGGCCCGCGGCGG113210.2431038788607853No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCT85160.18287012034082215No Hit
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATT82700.17758758750805534No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTGATTTAA82620.17741579782243688No Hit
GGACTTGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA80370.17258421291441844Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCTTTTGTAGTTCATGAGCGTGATGATTGGGTGTTCAT66100.14194122774223045No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG56140.1205534118827355No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATG51640.11089024206669865No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCG48890.10498497162356499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT564400.034.5080835
TCACGAA40200.033.12992530
GTGTAGA591550.033.02969734
CGTGTAG597300.032.7348133
GATCACG40650.032.6354728
ACGAAGG40600.032.6329232
CACGAAG40700.032.55128531
CGATGAA76750.032.3846835
CGAAGGT40350.032.35978333
ACGTGTA604500.032.3344332
GTTACTA41150.032.327635
GTGTTAC41000.032.1455933
ACTTACC45250.031.93193
GATACCA42400.031.91121520
ATCACGA41800.031.90437329
AACGTGT613650.031.8451731
ATACCAT42250.031.81594521
ACTATAC41950.031.6124844
TACTTAC46550.031.5655382
GGAGATA44450.031.34589417
Produced by FastQC (version 0.11.2)