FastQCFastQC Report
Mon 18 Jul 2022
rep1.r2.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep1.r2.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656333
Sequences flagged as poor quality0
Sequence length14-41
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT53308711.448644244301256Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2813106.041449355104113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1686783.6225501913200793No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA679811.4599686061971942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553481.1886606907194996No Hit
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTA375450.8063211973885888No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTG313870.6740712058179688No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG168150.36112107961350703No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT155390.33371754124973446No Hit
GACTTGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA142390.30579857583209796Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGGGGGAGAGGCTGTCGCTGCGCTTCTGGGCCCGCGGCG113210.24313123653312596No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCT85160.18289069961276394No Hit
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATT82700.17760757231065732No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTGATTTAA82620.17743576329270264No Hit
GGACTTGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA80370.1726036346627271Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCTTTTGTAGTTCATGAGCGTGATGATTGGGTGTTCAT66100.14195720108505985No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAA56140.12056697834970137No Hit
TACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT51640.11090272108975024No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTC48890.10499678609755787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT562450.053.9582335
GTTACTA42950.050.91695435
CGATGAA75850.050.71640835
CCCCATT13450.037.1468435
GATTTAA14300.035.702535
AGTATCT16650.035.58267235
GTGTAGA589700.034.82094234
TCGCCTT9000.033.36900335
CGAAGGT36750.033.20550533
CGTGTAG587500.033.1033933
ACGAAGG37150.032.84829332
TCGATGA78450.032.81189734
TGTTACT45250.032.70947334
TCACGAA38450.032.55266630
GATCACG37900.032.52076328
CACGAAG37750.032.24432831
CTACTCG19800.032.22259534
ACGTGTA604250.031.98626132
AACGTGT603850.031.94596131
GAACGTG606300.031.881430
Produced by FastQC (version 0.11.2)