FastQCFastQC Report
Sun 17 Jul 2022
rep1.r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep1.r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656857
Sequences flagged as poor quality0
Sequence length48
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCGT48547410.424928229490407No Hit
TCAAGTCAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGG975012.093708267185357No Hit
TCAAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT689061.47966750965297No Hit
TCAAGTCACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACG247310.531066339378684No Hit
TCAAGTCAAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGG219330.470982896833637No Hit
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCCACGAGACATCTCG137410.2950702587603613No Hit
CTCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCG130120.2794159236583816No Hit
TCAAGTCACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG123090.2643199050346618No Hit
TCAAGTCCAGGGGAAAGCGCGAACGCAGTCCCCCACTACCACAAATTA104720.22487269847452904No Hit
TCAAGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCG73450.157724405108424No Hit
AAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCT72340.15534082322046822No Hit
TCAAGTCGGTCAGACGGGTAATGTGCCCACGTCGTAACAAGGTTCAAG72140.15491134900642214No Hit
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACCGAGACATCTCG69750.14977913214857144No Hit
TCAAGTCCTGTCTCTTATACACCATCTCCGAGCCCACGAGACATCTCG65370.14037364686096224No Hit
TCAAGTCCTGTCTCTTATACACATCTCTGAGCCCACGAGACATCTCGT59830.12847721113188573No Hit
AAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAG58220.12501994370881475No Hit
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCATGAGACATCTCGT58090.12474078546968481No Hit
TCAAGTCCTGTCTCTTATACACATCTCCCGAGCCCACGAGACATCTCG57120.12265783553156132No Hit
AAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGG55910.1200595165365825No Hit
TCAAGTCAAACAGCAAGCTAGTCAAGCGCGGTTGTGGGGGGAGACAAT54510.11705319703825992No Hit
TCAAGTCCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGG51550.1106969786703779No Hit
TCAAGTCAGCCCCACGCCCGCCGCGGGCCCAGAAGCGCAGCGACAGCC48620.10440518143460277No Hit
CAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAG48200.10350328558510602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT567250.040.97743242
CAAGTCG1738650.040.0123442
AAGTCGC549800.039.931613
AAGTCGG582250.039.892513
AAGTCGA287500.039.7512553
AGTCGTT76100.039.746814
TCAGCGA110250.039.593256
AGTCGTA64600.039.584844
AGTCGCG88150.039.547734
AGTCGAC71950.039.5312544
AGTCGCT137150.039.5181244
GTCGCAT32050.039.5096175
TAGCGTT6600.039.49068516
TAGTCGC6600.039.48871240
AAGTCGT329250.039.4038123
AGTCGGC206900.039.377944
AGTCGAT44550.039.3562164
AGTCGTG80850.039.3393064
AGTCGGT85350.039.3074344
AGTCGGA160100.039.2585334
Produced by FastQC (version 0.11.2)