FastQCFastQC Report
Mon 18 Jul 2022
rep1.r1.trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamerep1.r1.trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4656333
Sequences flagged as poor quality0
Sequence length48
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCGT48546010.425800732035274No Hit
TCAAGTCAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGG975012.093943882449988No Hit
TCAAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT689061.479834023898205No Hit
TCAAGTCACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACG247310.5311261028796694No Hit
TCAAGTCAAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGG219330.4710358988500178No Hit
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCCACGAGACATCTCG137320.29491017931921965No Hit
CTCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCG130070.2793399870670762No Hit
TCAAGTCACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG123090.26434965025052976No Hit
TCAAGTCCAGGGGAAAGCGCGAACGCAGTCCCCCACTACCACAAATTA104720.22489800450268485No Hit
TCAAGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCG73450.1577421546096467No Hit
AAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCT72340.15535830448552543No Hit
TCAAGTCGGTCAGACGGGTAATGTGCCCACGTCGTAACAAGGTTCAAG72140.1549287819406387No Hit
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACCGAGACATCTCG69610.14949532174782174No Hit
TCAAGTCCTGTCTCTTATACACCATCTCCGAGCCCACGAGACATCTCG65230.14008877801480266No Hit
TCAAGTCCTGTCTCTTATACACATCTCTGAGCCCACGAGACATCTCGT59770.12836281253939527No Hit
AAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAG58220.1250340128165232No Hit
TCAAGTCCTGTCTCTTATACACATCTCCGAGCCCATGAGACATCTCGT58040.12464744252612518No Hit
TCAAGTCCTGTCTCTTATACACATCTCCCGAGCCCACGAGACATCTCG57090.12260721043791327No Hit
AAGTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGG55910.12007302742308164No Hit
TCAAGTCAAACAGCAAGCTAGTCAAGCGCGGTTGTGGGGGGAGACAAT54510.11706636960887462No Hit
TCAAGTCCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGG51550.11070943594455121No Hit
TCAAGTCAGCCCCACGCCCGCCGCGGGCCCAGAAGCGCAGCGACAGCC48620.10441693066196081No Hit
CAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGTGCCAG48200.1035149333176987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT562350.041.1043642
CAAGTCG1763250.040.0942572
AAGTCGG590850.040.012543
AAGTCGC556700.039.9220473
AGTCGTT77600.039.841084
AGTCGTG80400.039.831484
AGTCGGT88800.039.829414
AAGTCGA291050.039.8155753
AGTCGGC204250.039.8053634
TCAGCGA110300.039.7844626
AGTCGAT45900.039.7792784
AAGTCGT335850.039.5033453
AGTCGGA163750.039.502294
AGTCGTA68400.039.501114
AGTCGAC74150.039.3983844
AGTCGCG88400.039.3830344
GTCGCAT32700.039.3641365
AGTCGCT138200.039.3236244
GTCGTTC37100.039.2958345
GTCGACC31850.039.2525865
Produced by FastQC (version 0.11.2)