FastQCFastQC Report
Sun 29 Jan 2017
SRR1917544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1917544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30440416
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT15405650.041.07612644
TCTGCTT1036500.038.82312444
TTCGTAA47050.038.43355631
TAAGCCG46750.038.4003735
AAGCCGT46850.038.2714536
TCGTAAG47650.037.94960832
CGTAAGC48000.037.6735133
TGTTCGA56300.036.9250530
AATGCCG54650.036.6737336
TTCGAAT56450.036.554232
GTTCGAA57050.036.4396831
CGTATGA29350.036.27795434
TATGACG29300.036.26656336
AGCCGTC45850.036.13121437
TTCGGAT33250.035.9919731
CGAATGC56000.035.94500734
TCGGATG33400.035.76446532
ATGCCGC44150.034.7817737
GACGTCT30600.034.72816539
TGTTCGG35250.034.44907829
Produced by FastQC (version 0.11.5)