FastQCFastQC Report
Sun 29 Jan 2017
SRR1917541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1917541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61472199
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT29723550.041.54390744
TTCGGAT45500.033.49976731
ATGCCGG90450.032.49007836
TCGGATG46600.032.42601832
TCTGCTT1663250.032.06625444
TTCGAAT41850.031.1660732
GGTTCGG51500.030.23650229
AATGCCG41500.029.94699136
GTTCGGA52350.029.66193830
ATTAAGT174400.029.25789618
ATTGAGT171550.028.55164518
GTTCGAA46850.028.54394331
GGTTCGA47650.028.52575330
CGAGAGT134600.028.36653517
GATGCCG50900.028.13300135
ATTTAGT406800.028.0492118
CGAATGC44700.027.85234534
ATGCCGC63550.027.37876137
ATTCAGT386150.027.1854818
ATCAAGT218050.027.18343518
Produced by FastQC (version 0.11.5)