FastQCFastQC Report
Sun 29 Jan 2017
SRR1917540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1917540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86508988
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT42727300.041.56653644
TCTGCTT2378450.038.95857244
TTCGGAT262600.037.55573331
TCGGATG265050.037.1836932
GTTCGGA269850.036.79057730
GATGCCG233400.036.3539935
CGGATGC234150.036.27510533
GGTTCGG278950.036.22826829
TCGTAGG80150.033.18377733
CGTAGGC85600.031.0453434
TAGGCCG80750.030.86673436
TTCGTAG87050.030.55341532
ATGCCGG173950.030.52924736
GGATGCC278300.030.4966634
AGGCCGT84900.029.12452537
GGCCGTC89450.027.66826838
TGCCGGC194250.027.42921437
CGAGAGT175650.027.10195217
TCGTACG6450.026.60344533
CCGGCTT201200.026.56049539
Produced by FastQC (version 0.11.5)